ID   WTC CRISPRi HOPX g4
AC   CVCL_VQ28
DR   Wikidata; Q98135034
RX   PubMed=30290179;
CC   Population: Japanese.
CC   Characteristics: Cell line with Dox-inducible CRISPR interference (CRISPRi).
CC   Characteristics: Using TALEN a construct containing a rtTA+TRE3G promoter and dCas9-KRAB-P2A-mCherry was introduced in the AAVS1 safe harbor locus.
CC   Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:24961; HOPX.
CC   Genetic integration: Method=TALEN; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
CC   Genetic integration: Method=TALEN; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Genetic integration: Method=TALEN; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
CC   Genetic integration: Method=TALEN; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala and KRAB domain = dCas9-KRAB).
CC   Derived from site: In situ; Leg, skin; UBERON=UBERON_0001511.
CC   Cell type: Fibroblast of skin; CL=CL_0002620.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_VM38 ! CRISPRi Gen1C 5
SX   Male
AG   30Y
CA   Induced pluripotent stem cell
DT   Created: 07-09-18; Last updated: 10-04-25; Version: 14
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RX   PubMed=30290179; DOI=10.1016/j.stem.2018.09.009; PMCID=PMC6220122;
RA   Friedman C.E., Nguyen Q., Lukowski S.W., Helfer A., Chiu H.S., Miklas J.,
RA   Levy S., Suo S.-B., Han J.-D.J., Osteil P., Peng G.-D., Jing N.-H.,
RA   Baillie G.J., Senabouth A., Christ A.N., Bruxner T.J.C., Murry C.E.,
RA   Wong E.S., Ding J., Wang Y.-L., Hudson J., Ruohola-Baker H.,
RA   Bar-Joseph Z., Tam P.P.L., Powell J.E., Palpant N.J.;
RT   "Single-cell transcriptomic analysis of cardiac differentiation from
RT   human PSCs reveals HOPX-dependent cardiomyocyte maturation.";
RL   Cell Stem Cell 23:586-598.e8(2018).
//