ID   CRISPRi Gen1C 5
AC   CVCL_VM38
SY   WTC CRISPRi Gen1 clone 5; CRISPRi (Gen1C 5); 1C-5
DR   Wikidata; Q93458763
RX   PubMed=26971820;
RX   PubMed=30290179;
CC   Population: Japanese.
CC   Characteristics: Cell line with Dox-inducible CRISPR interference (CRISPRi).
CC   Characteristics: Using TALEN a construct containing a rtTA+TRE3G promoter and dCas9-KRAB-P2A-mCherry is introduced in the AAVS1 safe harbor locus.
CC   Genetic integration: Method=TALEN; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
CC   Genetic integration: Method=TALEN; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Genetic integration: Method=TALEN; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
CC   Genetic integration: Method=TALEN; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala and KRAB domain = dCas9-KRAB).
CC   Derived from site: In situ; Leg, skin; UBERON=UBERON_0001511.
CC   Cell type: Fibroblast of skin; CL=CL_0002620.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_Y803 ! GM25256
SX   Male
AG   30Y
CA   Induced pluripotent stem cell
DT   Created: 07-09-18; Last updated: 10-04-25; Version: 12
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RX   PubMed=26971820; DOI=10.1016/j.stem.2016.01.022; PMCID=PMC4830697;
RA   Mandegar M.A., Huebsch N., Frolov E.B., Shin E., Truong A.,
RA   Olvera M.P., Chan A.H., Miyaoka Y., Holmes K., Spencer C.I.,
RA   Judge L.M., Gordon D.E., Eskildsen T.V., Villalta J.E., Horlbeck M.A.,
RA   Gilbert L.A., Krogan N.J., Sheikh S.P., Weissman J.S., Qi L.S.,
RA   So P.-L., Conklin B.R.;
RT   "CRISPR interference efficiently induces specific and reversible gene
RT   silencing in human iPSCs.";
RL   Cell Stem Cell 18:541-553(2016).
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RX   PubMed=30290179; DOI=10.1016/j.stem.2018.09.009; PMCID=PMC6220122;
RA   Friedman C.E., Nguyen Q., Lukowski S.W., Helfer A., Chiu H.S., Miklas J.,
RA   Levy S., Suo S.-B., Han J.-D.J., Osteil P., Peng G.-D., Jing N.-H.,
RA   Baillie G.J., Senabouth A., Christ A.N., Bruxner T.J.C., Murry C.E.,
RA   Wong E.S., Ding J., Wang Y.-L., Hudson J., Ruohola-Baker H.,
RA   Bar-Joseph Z., Tam P.P.L., Powell J.E., Palpant N.J.;
RT   "Single-cell transcriptomic analysis of cardiac differentiation from
RT   human PSCs reveals HOPX-dependent cardiomyocyte maturation.";
RL   Cell Stem Cell 23:586-598.e8(2018).
//