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Cellosaurus WTC CRISPRi HOPX g1 (CVCL_VQ27)

[Text version]
Cell line name WTC CRISPRi HOPX g1
Accession CVCL_VQ27
Resource Identification Initiative To cite this cell line use: WTC CRISPRi HOPX g1 (RRID:CVCL_VQ27)
Comments Population: Japanese.
Characteristics: Cell line with Dox-inducible CRISPR interference (CRISPRi).
Characteristics: Using TALEN a construct containing a rtTA+TRE3G promoter and dCas9-KRAB-P2A-mCherry was introduced in the AAVS1 safe harbor locus.
Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:24961; HOPX.
Genetic integration: Method=TALEN; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
Genetic integration: Method=TALEN; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Genetic integration: Method=TALEN; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
Genetic integration: Method=TALEN; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala and KRAB domain = dCas9-KRAB).
Derived from site: In situ; Leg, skin; UBERON=UBERON_0001511.
Cell type: Fibroblast of skin; CL=CL_0002620.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_VM38 (CRISPRi Gen1C 5)
Sex of cell Male
Age at sampling 30Y
Category Induced pluripotent stem cell
Publications

PubMed=30290179; DOI=10.1016/j.stem.2018.09.009; PMCID=PMC6220122
Friedman C.E., Nguyen Q., Lukowski S.W., Helfer A., Chiu H.S., Miklas J., Levy S., Suo S.-B., Han J.-D.J., Osteil P., Peng G.-D., Jing N.-H., Baillie G.J., Senabouth A., Christ A.N., Bruxner T.J.C., Murry C.E., Wong E.S., Ding J., Wang Y.-L., Hudson J., Ruohola-Baker H., Bar-Joseph Z., Tam P.P.L., Powell J.E., Palpant N.J.
Single-cell transcriptomic analysis of cardiac differentiation from human PSCs reveals HOPX-dependent cardiomyocyte maturation.
Cell Stem Cell 23:586-598.e8(2018)

Cross-references
Encyclopedic resources Wikidata; Q98135031
Entry history
Entry creation07-Sep-2018
Last entry update10-Apr-2025
Version number14