Cellosaurus cell line H9-iCas9 (CVCL

Cellosaurus H9-iCas9 (CVCL_E7G4)

[Text version]

Cell line name

H9-iCas9

Accession

CVCL_E7G4

Resource Identification Initiative

To cite this cell line use: H9-iCas9 (RRID:CVCL_E7G4)

Comments

Characteristics: Cell line with robust Dox-inducible expression of Cas9 (PubMed=38917806).
Characteristics: Using TALEN two constructs SA-2A-HygR-Cas9-TRE and SA-2A-Neo-M2rtTA were introduced in the AAVS1 safe harbor locus, one in each allele (PubMed=38917806).
Genetic integration: Method=TALEN; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=TALEN; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Genetic integration: Method=TALEN; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.

Species of origin

Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy

Parent: CVCL_9773 (WA09)
Children:

CVCL_E7G5 (H9-iCas9-APP(swe/swe))

Sex of cell

Female

Age at sampling

Blastocyst stage