Cellosaurus H9-iCas9-APP(swe/swe) (CVCL_E7G5)
Cell line name | H9-iCas9-APP(swe/swe) |
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Synonyms | H9-iCas9-APPswe/swe |
Accession | CVCL_E7G5 |
Resource Identification Initiative | To cite this cell line use: H9-iCas9-APP(swe/swe) (RRID:CVCL_E7G5) |
Comments | Characteristics: Cell line with robust Dox-inducible expression of Cas9 and an edited homozygous APP(Swe) mutation (PubMed=38917806). Characteristics: Using TALEN two constructs SA-2A-HygR-Cas9-TRE and SA-2A-Neo-M2rtTA were introduced in the AAVS1 safe harbor locus, one in each allele (from parent cell line). Genetic integration: Method=TALEN; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Genetic integration: Method=TALEN; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9. Genetic integration: Method=TALEN; Gene=UniProtKB; P00552; Transposon Tn5 neo. Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358. Cell type: Embryonic stem cell; CL=CL_0002322. |
Sequence variations |
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Disease | Alzheimer's disease 1 (NCIt: C146894) Early-onset autosomal dominant Alzheimer disease (ORDO: Orphanet_1020) |
Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
Hierarchy | Parent: CVCL_E7G4 (H9-iCas9) |
Sex of cell | Female |
Age at sampling | Blastocyst stage |
Category | Embryonic stem cell |
Publications | PubMed=38917806; DOI=10.1016/j.stem.2024.06.001; PMCID=PMC11405001 |
Entry history | |
Entry creation | 10-Apr-2025 |
Last entry update | 10-Apr-2025 |
Version number | 1 |