ID   H9-iCas9
AC   CVCL_E7G4
RX   PubMed=38917806;
CC   Characteristics: Cell line with robust Dox-inducible expression of Cas9 (PubMed=38917806).
CC   Characteristics: Using TALEN two constructs SA-2A-HygR-Cas9-TRE and SA-2A-Neo-M2rtTA were introduced in the AAVS1 safe harbor locus, one in each allele (PubMed=38917806).
CC   Genetic integration: Method=TALEN; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=TALEN; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
CC   Genetic integration: Method=TALEN; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_9773 ! WA09
SX   Female
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 10-04-25; Last updated: 10-04-25; Version: 1
//
RX   PubMed=38917806; DOI=10.1016/j.stem.2024.06.001; PMCID=PMC11405001;
RA   Saurat N., Minotti A.P., Rahman M.T., Sikder T., Zhang C., Cornacchia D.,
RA   Jungverdorben J., Ciceri G., Betel D., Studer L.P.;
RT   "Genome-wide CRISPR screen identifies neddylation as a regulator of
RT   neuronal aging and AD neurodegeneration.";
RL   Cell Stem Cell 31:1162-1174.e8(2024).
//