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Cellosaurus BTI-Tn-5B1-4 (CVCL_C190)

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Cell line name BTI-Tn-5B1-4
Synonyms BTI-TN-5B1-4; BTI-TN5B1-4; BTI-Tn5B14; BTI-Tn 5B1-4; Tn-5B1-4; Tn 5B1-4; Tn5 B1-4; Tn5B1-4; TN5B14; TnH5; High Five; High 5; High-5; High5; Hi-five; Hi-5; Hi5; Tn-5
Accession CVCL_C190
Resource Identification Initiative To cite this cell line use: BTI-Tn-5B1-4 (RRID:CVCL_C190)
Comments Group: Insect cell line.
Group: Patented cell line.
Group: Recombinant protein production insect cell line.
Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-10859.
Virology: Infected with alphanodavirus TnCLV.
Doubling time: 26 hours (DOI=10.1111/j.1744-7917.2004.tb00236.x; PubMed=21667340); 34 +- 1 hours (Note=In static culture), 27 +- 2 hours (Note=In suspension culture) (PubMed=10585188).
Omics: Genomics; Whole genome sequencing.
Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
Species of origin Trichoplusia ni (Cabbage looper) (NCBI Taxonomy: 7111)
Hierarchy Parent: CVCL_Z410 (BTI-Tn-5B1-28)
Children:
CVCL_Z252 (BTI-Tnao38)CVCL_B6BW (H5-GFP)CVCL_Z094 (H5CL-B)
CVCL_Z095 (H5CL-F)CVCL_RW20 (Hi-5 Rix4446)CVCL_UF19 (High5-ht33)
CVCL_UF20 (High5-ht35)CVCL_Z431 (Tn-4h)CVCL_A8XU (Tn5B-40)
CVCL_RY32 (Tni-FNL)CVCL_RT23 (Tnms42)
Sex of cell Sex unspecified
Age at sampling Embryonic stage
Category Spontaneously immortalized cell line
Web pages Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/h/cell-lines-detail-175.html
Info; Wikipedia; -; https://en.wikipedia.org/wiki/High_Five_cells
Provider; Thermo Fisher; B85502; https://www.thermofisher.com/order/catalog/product/B85502
Publications

PubMed=1369220; DOI=10.1021/bp00017a003
Thomas J. Wickham, Thomas R. Davis, Robert R. Granados, Michael Louis Shuler, H. Alan Wood;
Screening of insect cell lines for the production of recombinant proteins and infectious virus in the baculovirus expression system.
Biotechnol. Prog. 8:391-396(1992)

PubMed=8314732; DOI=10.1007/BF02633986
Thomas R. Davis, Thomas J. Wickham, Kevin A. McKenna, Robert R. Granados, Michael Louis Shuler, H. Alan Wood;
Comparative recombinant protein production of eight insect cell lines.
In Vitro Cell. Dev. Biol. Anim. 29:388-390(1993)

DOI=10.1016/S0022-2011(94)90400-6
Robert R. Granados, Guo-Xun Li, Anja C.G. Derksen, Kevin A. McKenna;
A new insect cell line from Trichoplusia ni (BTI-Tn-5B1-4) susceptible to Trichoplusia ni single enveloped nuclear polyhedrosis virus.
J. Invertebr. Pathol. 64:260-266(1994)

Patent=US5300435
Robert R. Granados;
Trichoplusia ni cell line which supports replication of other baculoviruses.
Patent number US5300435, 05-Apr-1994

PubMed=10358729
Tohru Yanase, Chisa Yasunaga, Takeshi Kawarabata;
Replication of Spodoptera exigua nucleopolyhedrovirus in permissive and non-permissive lepidopteran cell lines.
Acta Virol. 42:293-298(1998)

PubMed=10397817; DOI=10.1002/(SICI)1097-0290(19990605)63:5<612::AID-BIT11>3.0.CO;2-C
Mark A. Saarinen, Kimberly A. Troutner, Steve G. Gladden, Christine M. Mitchell-Logean, David W. Murhammer;
Recombinant protein synthesis in Trichoplusia ni BTI-Tn-5B1-4 insect cell aggregates.
Biotechnol. Bioeng. 63:612-617(1999)

PubMed=10585188; DOI=10.1021/bp990119f
Michelle B. Keith, Patrick J. Farrell, Kostas Iatrou, Leo Augustus Behie;
Screening of transformed insect cell lines for recombinant protein production.
Biotechnol. Prog. 15:1046-1052(1999)

DOI=10.1111/j.1744-7917.2004.tb00236.x
Jian-Xiao Tian, Chang-You Li, Gui-Ling Zheng, Guo-Xun Li, Ping Wang, Robert R. Granados;
A new cell clone derived from Trichoplusia ni TN5B1-4 cells.
Entomol. Sin. 11:165-171(2004)

Patent=US7179648
Robert R. Granados, Guo-Xun Li;
Clonal cell lines derived from BTI-TN-5B1-4.
Patent number US7179648, 20-Feb-2007

PubMed=17686877; DOI=10.1128/JVI.00807-07; PMCID=PMC2045576
Tian-Cheng Li, Paul D. Scotti, Tatsuo Miyamura, Naokazu Takeda;
Latent infection of a new alphanodavirus in an insect cell line.
J. Virol. 81:10890-10896(2007)

PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022
Axel Karger, Barbara Bettin, Matthias Lenk, Thomas Christoph Mettenleiter;
Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing.
J. Virol. Methods 164:116-121(2010)

PubMed=20300881; DOI=10.1007/s12033-010-9268-3; PMCID=PMC4388404
Florian Krammer, Theresa Schinko, Dieter Palmberger, Christopher Tauer, Paul Messner, Reingard Grabherr;
Trichoplusia ni cells (High Five) are highly efficient for the production of influenza A virus-like particles: a comparison of two insect cell lines as production platforms for influenza vaccines.
Mol. Biotechnol. 45:226-234(2010)

PubMed=21667340; DOI=10.1007/s12250-011-3177-x; PMCID=PMC8222434
Yan-Lei Wu, Lei Jiang, Yoshifumi Hashimoto, Robert R. Granados, Guo-Xun Li;
Establishment, growth kinetics, and susceptibility to AcMNPV of heat tolerant lepidopteran cell lines.
Virol. Sin. 26:198-205(2011)

PubMed=23848607; DOI=10.1021/pr400329k; PMCID=PMC3800089
Yan-Ming An, Joseph A. Rininger, Donald L. Jarvis, Xiang-Hong Jing, Zhi-Ping Ye, Jared James Aumiller, Maryna C. Eichelberger, John F. Cipollo;
Comparative glycomics analysis of influenza Hemagglutinin (H5N1) produced in vaccine relevant cell platforms.
J. Proteome Res. 12:3707-3720(2013)

PubMed=24375231; DOI=10.1007/s10529-013-1429-6; PMCID=PMC3955137
Monika Wilde, Miriam Klausberger, Dieter Palmberger, Wolfgang Ernst, Reingard Grabherr;
Tnao38, high five and Sf9 -- evaluation of host-virus interactions in three different insect cell lines: baculovirus production and recombinant protein expression.
Biotechnol. Lett. 36:743-749(2014)

PubMed=29133148; DOI=10.1016/j.pep.2017.11.002; PMCID=PMC5826799
Christoph Geisler, Donald L. Jarvis;
Adventitious viruses in insect cell lines used for recombinant protein expression.
Protein Expr. Purif. 144:25-32(2018)

PubMed=29376823; DOI=10.7554/eLife.31628; PMCID=PMC5844692
Yu Fu, Yu-Jing Yang, Han Zhang, Gwen Farley, Jun-Ling Wang, Kaycee A. Quarles, Zhi-Ping Weng, Phillip D. Zamore;
The genome of the Hi5 germ cell line from Trichoplusia ni, an agricultural pest and novel model for small RNA biology.
eLife 7:e31628.1-e31628.41(2018)

PubMed=30449074; DOI=10.1111/1755-0998.12966
Wen-Bo Chen, Xiao-Wei Yang, Guillaume Tetreau, Xiao-Zhao Song, Cathy Coutu, Dwayne Hegedus, Gary W. Blissard, Zhang-Jun Fei, Ping Wang;
A high-quality chromosome-level genome assembly of a generalist herbivore, Trichoplusia ni.
Mol. Ecol. Resour. 19:485-496(2019)

Cross-references
Cell line collections (Providers) CCLV; CCLV-RIE 0350
CCTCC; GDC0293
ECACC; 94020901 - Discontinued
Cell line databases/resources CLO; CLO_0009378
CLDB; cl4535
CCRID; 4201INS-CCTCC00293
ICLDB; bti-tn-5b1-4
Lonza; 1180
TOKU-E; 4051
TOKU-E; 4129
Anatomy/cell type resources BTO; BTO_0003619
Chemistry resources ChEMBL-Cells; CHEMBL3833782
PubChem_Cell_line; CVCL_C190
Encyclopedic resources Wikidata; Q17126384
Experimental variables resources EFO; EFO_0022724
Entry history
Entry creation06-Jun-2012
Last entry update14-Aug-2025
Version number32