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Cellosaurus BTI-Tn-5B1-4 (CVCL_C190)

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Cell line name BTI-Tn-5B1-4
Synonyms BTI-TN-5B1-4; BTI-TN5B1-4; BTI-Tn5B14; BTI-Tn 5B1-4; Tn-5B1-4; Tn 5B1-4; Tn5 B1-4; Tn5B1-4; TN5B14; TnH5; High Five; High 5; High-5; High5; Hi-five; Hi-5; Hi5; Tn-5
Accession CVCL_C190
Resource Identification Initiative To cite this cell line use: BTI-Tn-5B1-4 (RRID:CVCL_C190)
Comments Group: Insect cell line.
Group: Patented cell line.
Group: Recombinant protein production insect cell line.
Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-10859.
Virology: Infected with alphanodavirus TnCLV.
Doubling time: 26 hours (DOI=10.1111/j.1744-7917.2004.tb00236.x; PubMed=21667340); 34 +- 1 hours (Note=In static culture), 27 +- 2 hours (Note=In suspension culture) (PubMed=10585188).
Omics: Genome sequenced.
Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
Species of origin Trichoplusia ni (Cabbage looper) (NCBI Taxonomy: 7111)
Hierarchy Parent: CVCL_Z410 (BTI-Tn-5B1-28)
CVCL_Z252 (BTI-Tnao38)CVCL_B6BW (H5-GFP)CVCL_Z094 (H5CL-B)
CVCL_Z095 (H5CL-F)CVCL_RW20 (Hi-5 Rix4446)CVCL_UF19 (High5-ht33)
CVCL_UF20 (High5-ht35)CVCL_Z431 (Tn-4h)CVCL_A8XU (Tn5B-40)
CVCL_RY32 (Tni-FNL)CVCL_RT23 (Tnms42)
Sex of cell Sex unspecified
Age at sampling Embryonic stage
Category Spontaneously immortalized cell line
Web pages https://en.wikipedia.org/wiki/High_Five_cells

PubMed=1369220; DOI=10.1021/bp00017a003
Wickham T.J., Davis T., Granados R.R., Shuler M.L., Wood H.A.
Screening of insect cell lines for the production of recombinant proteins and infectious virus in the baculovirus expression system.
Biotechnol. Prog. 8:391-396(1992)

PubMed=8314732; DOI=10.1007/BF02633986
Davis T.R., Wickham T.J., McKenna K.A., Granados R.R., Shuler M.L., Wood H.A.
Comparative recombinant protein production of eight insect cell lines.
In Vitro Cell. Dev. Biol. Anim. 29:388-390(1993)

Granados R.R., Li G.-X., Derksen A.C.G., McKenna K.A.
A new insect cell line from Trichoplusia ni (BTI-Tn-5B1-4) susceptible to Trichoplusia ni single enveloped nuclear polyhedrosis virus.
J. Invertebr. Pathol. 64:260-266(1994)

Granados R.R.
Trichoplusia ni cell line which supports replication of other baculoviruses.
Patent number US5300435, 05-Apr-1994

Yanase T., Yasunaga C., Kawarabata T.
Replication of Spodoptera exigua nucleopolyhedrovirus in permissive and non-permissive lepidopteran cell lines.
Acta Virol. 42:293-298(1998)

PubMed=10397817; DOI=10.1002/(SICI)1097-0290(19990605)63:5<612::AID-BIT11>3.0.CO;2-C
Saarinen M.A., Troutner K.A., Gladden S.G., Mitchell-Logean C.M., Murhammer D.W.
Recombinant protein synthesis in Trichoplusia ni BTI-Tn-5B1-4 insect cell aggregates.
Biotechnol. Bioeng. 63:612-617(1999)

PubMed=10585188; DOI=10.1021/bp990119f
Keith M.B., Farrell P.J., Iatrou K., Behie L.A.
Screening of transformed insect cell lines for recombinant protein production.
Biotechnol. Prog. 15:1046-1052(1999)

Tian J.-X., Li C.-Y., Zheng G.-L., Li G.-X., Wang P., Granados R.R.
A new cell clone derived from Trichoplusia ni TN5B1-4 cells.
Entomol. Sin. 11:165-171(2004)

Granados R.R., Li G.-X.
Clonal cell lines derived from BTI-TN-5B1-4.
Patent number US7179648, 20-Feb-2007

PubMed=17686877; DOI=10.1128/JVI.00807-07
Li T.-C., Scotti P.D., Miyamura T., Takeda N.
Latent infection of a new alphanodavirus in an insect cell line.
J. Virol. 81:10890-10896(2007)

PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022
Karger A., Bettin B., Lenk M., Mettenleiter T.C.
Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing.
J. Virol. Methods 164:116-121(2010)

PubMed=20300881; DOI=10.1007/s12033-010-9268-3
Krammer F., Schinko T., Palmberger D., Tauer C., Messner P., Grabherr R.
Trichoplusia ni cells (High Five) are highly efficient for the production of influenza A virus-like particles: a comparison of two insect cell lines as production platforms for influenza vaccines.
Mol. Biotechnol. 45:226-234(2010)

PubMed=21667340; DOI=10.1007/s12250-011-3177-x
Wu Y.-L., Jiang L., Hashimoto Y., Granados R.R., Li G.-X.
Establishment, growth kinetics, and susceptibility to AcMNPV of heat tolerant lepidopteran cell lines.
Virol. Sin. 26:198-205(2011)

PubMed=23848607; DOI=10.1021/pr400329k
An Y.-M., Rininger J.A., Jarvis D.L., Jing X.-H., Ye Z.-P., Aumiller J.J., Eichelberger M.C., Cipollo J.F.
Comparative glycomics analysis of influenza Hemagglutinin (H5N1) produced in vaccine relevant cell platforms.
J. Proteome Res. 12:3707-3720(2013)

PubMed=24375231; DOI=10.1007/s10529-013-1429-6
Wilde M., Klausberger M., Palmberger D., Ernst W., Grabherr R.
Tnao38, high five and Sf9 -- evaluation of host-virus interactions in three different insect cell lines: baculovirus production and recombinant protein expression.
Biotechnol. Lett. 36:743-749(2014)

PubMed=29133148; DOI=10.1016/j.pep.2017.11.002
Geisler C., Jarvis D.L.
Adventitious viruses in insect cell lines used for recombinant protein expression.
Protein Expr. Purif. 144:25-32(2017)

PubMed=29376823; DOI=10.7554/eLife.31628
Fu Y., Yang Y.-J., Zhang H., Farley G., Wang J.-L., Quarles K.A., Weng Z.-P., Zamore P.D.
The genome of the Hi5 germ cell line from Trichoplusia ni, an agricultural pest and novel model for small RNA biology.
eLife 7:e31628.1-e31628.41(2018)

PubMed=30449074; DOI=10.1111/1755-0998.12966
Chen W.-B., Yang X.-W., Tetreau G., Song X.-Z., Coutu C., Hegedus D., Blissard G.W., Fei Z.-J., Wang P.
A high-quality chromosome-level genome assembly of a generalist herbivore, Trichoplusia ni.
Mol. Ecol. Resour. 19:485-496(2019)

Cell line collections (Providers) CCLV; CCLV-RIE 0350
ECACC; 94020901 - Discontinued
Cell line databases/resources CLO; CLO_0009378
CLDB; cl4535
ICLDB; bti-tn-5b1-4
Lonza; 1180
TOKU-E; 4051
TOKU-E; 4129
Anatomy/cell type resources BTO; BTO:0003619
Chemistry resources ChEMBL-Cells; CHEMBL3833782
PubChem_Cell_line; CVCL_C190
Encyclopedic resources Wikidata; Q17126384
Entry history
Entry creation06-Jun-2012
Last entry update29-Jun-2023
Version number28