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Cellosaurus BTI-Tnao38 (CVCL_Z252)

[Text version]
Cell line name BTI-Tnao38
Synonyms Tnao38; Ao38
Accession CVCL_Z252
Resource Identification Initiative To cite this cell line use: BTI-Tnao38 (RRID:CVCL_Z252)
Comments Group: Insect cell line.
Group: Recombinant protein production insect cell line.
Problematic cell line: Contaminated. Originally thought to be from Ascalapha odorata. Shown to be a BTI-Tn-5B1-4 derivative (PubMed=22531032).
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00510.
Doubling time: 20.2 hours (PubMed=20602790).
Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
Species of origin Trichoplusia ni (Cabbage looper) (NCBI Taxonomy: 7111)
Hierarchy Parent: CVCL_C190 (BTI-Tn-5B1-4)
Sex of cell Sex unspecified
Age at sampling Embryonic stage
Category Spontaneously immortalized cell line
Web pages https://iclac.org/wp-content/uploads/Cross-Contaminations_v12_distribution.xlsx

PubMed=20602790; DOI=10.1186/1472-6750-10-50
Hashimoto Y., Zhang S., Blissard G.W.
Ao38, a new cell line from eggs of the black witch moth, Ascalapha odorata (Lepidoptera: Noctuidae), is permissive for AcMNPV infection and produces high levels of recombinant proteins.
BMC Biotechnol. 10:50.1-50.16(2010)

PubMed=22531032; DOI=10.1186/1472-6750-12-12
Hashimoto Y., Zhang S., Zhang S.-Y., Chen Y.-R., Blissard G.W.
Correction: BTI-Tnao38, a new cell line derived from Trichoplusia ni, is permissive for AcMNPV infection and produces high levels of recombinant proteins.
BMC Biotechnol. 12:12.1-12.4(2012)

PubMed=24375231; DOI=10.1007/s10529-013-1429-6
Wilde M., Klausberger M., Palmberger D., Ernst W., Grabherr R.
Tnao38, high five and Sf9 -- evaluation of host-virus interactions in three different insect cell lines: baculovirus production and recombinant protein expression.
Biotechnol. Lett. 36:743-749(2014)

PubMed=29133148; DOI=10.1016/j.pep.2017.11.002
Geisler C., Jarvis D.L.
Adventitious viruses in insect cell lines used for recombinant protein expression.
Protein Expr. Purif. 144:25-32(2017)

Cell line collections (Providers) ABM; T0853
Cell line databases/resources ICLDB; bti-tnao38
Biological sample resources BioSample; SAMN06481107
Encyclopedic resources Wikidata; Q54798603
Entry history
Entry creation10-Apr-2015
Last entry update30-Jan-2024
Version number15