ID   BTI-Tn-5B1-4
AC   CVCL_C190
SY   BTI-TN-5B1-4; BTI-TN5B1-4; BTI-Tn5B14; BTI-Tn 5B1-4; Tn-5B1-4; Tn 5B1-4; Tn5 B1-4; Tn5B1-4; TN5B14; TnH5; High Five; High 5; High-5; High5; Hi-five; Hi-5; Hi5; Tn-5
DR   BTO; BTO:0003619
DR   CLO; CLO_0009378
DR   CLDB; cl4535
DR   CCRID; 4201INS-CCTCC00293
DR   ChEMBL-Cells; CHEMBL3833782
DR   ECACC; 94020901
DR   ICLDB; bti-tn-5b1-4
DR   Lonza; 1180
DR   PubChem_Cell_line; CVCL_C190
DR   TOKU-E; 4051
DR   TOKU-E; 4129
DR   Wikidata; Q17126384
RX   DOI=10.1016/S0022-2011(94)90400-6;
RX   DOI=10.1111/j.1744-7917.2004.tb00236.x;
RX   Patent=US5300435;
RX   Patent=US7179648;
RX   PubMed=1369220;
RX   PubMed=8314732;
RX   PubMed=10358729;
RX   PubMed=10397817;
RX   PubMed=10585188;
RX   PubMed=19941903;
RX   PubMed=17686877;
RX   PubMed=20300881;
RX   PubMed=21667340;
RX   PubMed=23848607;
RX   PubMed=24375231;
RX   PubMed=29133148;
RX   PubMed=29376823;
RX   PubMed=30449074;
CC   Group: Insect cell line.
CC   Group: Patented cell line.
CC   Group: Recombinant protein production insect cell line.
CC   Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-10859.
CC   Virology: Infected with alphanodavirus TnCLV.
CC   Doubling time: 26 hours (DOI=10.1111/j.1744-7917.2004.tb00236.x; PubMed=21667340); 34 +- 1 hours (Note=In static culture), 27 +- 2 hours (Note=In suspension culture) (PubMed=10585188).
CC   Omics: Genome sequenced.
CC   Discontinued: ECACC; 94020901; probable.
CC   Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
OX   NCBI_TaxID=7111; ! Trichoplusia ni (Cabbage looper)
HI   CVCL_Z410 ! BTI-Tn-5B1-28
SX   Sex unspecified
AG   Embryonic stage
CA   Spontaneously immortalized cell line
DT   Created: 06-06-12; Last updated: 29-06-23; Version: 28
RX   DOI=10.1016/S0022-2011(94)90400-6;
RA   Granados R.R., Li G.-X., Derksen A.C.G., McKenna K.A.;
RT   "A new insect cell line from Trichoplusia ni (BTI-Tn-5B1-4)
RT   susceptible to Trichoplusia ni single enveloped nuclear polyhedrosis
RT   virus.";
RL   J. Invertebr. Pathol. 64:260-266(1994).
RX   DOI=10.1111/j.1744-7917.2004.tb00236.x;
RA   Tian J.-X., Li C.-Y., Zheng G.-L., Li G.-X., Wang P., Granados R.R.;
RT   "A new cell clone derived from Trichoplusia ni TN5B1-4 cells.";
RL   Entomol. Sin. 11:165-171(2004).
RX   Patent=US5300435;
RA   Granados R.R.;
RT   "Trichoplusia ni cell line which supports replication of other
RT   baculoviruses.";
RL   Patent number US5300435, 05-Apr-1994.
RX   Patent=US7179648;
RA   Granados R.R., Li G.-X.;
RT   "Clonal cell lines derived from BTI-TN-5B1-4.";
RL   Patent number US7179648, 20-Feb-2007.
RX   PubMed=1369220; DOI=10.1021/bp00017a003;
RA   Wickham T.J., Davis T., Granados R.R., Shuler M.L., Wood H.A.;
RT   "Screening of insect cell lines for the production of recombinant
RT   proteins and infectious virus in the baculovirus expression system.";
RL   Biotechnol. Prog. 8:391-396(1992).
RX   PubMed=8314732; DOI=10.1007/BF02633986;
RA   Davis T.R., Wickham T.J., McKenna K.A., Granados R.R., Shuler M.L.,
RA   Wood H.A.;
RT   "Comparative recombinant protein production of eight insect cell
RT   lines.";
RL   In Vitro Cell. Dev. Biol. Anim. 29:388-390(1993).
RX   PubMed=10358729;
RA   Yanase T., Yasunaga C., Kawarabata T.;
RT   "Replication of Spodoptera exigua nucleopolyhedrovirus in permissive
RT   and non-permissive lepidopteran cell lines.";
RL   Acta Virol. 42:293-298(1998).
RX   PubMed=10397817; DOI=10.1002/(SICI)1097-0290(19990605)63:5<612::AID-BIT11>3.0.CO;2-C;
RA   Saarinen M.A., Troutner K.A., Gladden S.G., Mitchell-Logean C.M.,
RA   Murhammer D.W.;
RT   "Recombinant protein synthesis in Trichoplusia ni BTI-Tn-5B1-4 insect
RT   cell aggregates.";
RL   Biotechnol. Bioeng. 63:612-617(1999).
RX   PubMed=10585188; DOI=10.1021/bp990119f;
RA   Keith M.B., Farrell P.J., Iatrou K., Behie L.A.;
RT   "Screening of transformed insect cell lines for recombinant protein
RT   production.";
RL   Biotechnol. Prog. 15:1046-1052(1999).
RX   PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022;
RA   Karger A., Bettin B., Lenk M., Mettenleiter T.C.;
RT   "Rapid characterisation of cell cultures by matrix-assisted laser
RT   desorption/ionisation mass spectrometric typing.";
RL   J. Virol. Methods 164:116-121(2010).
RX   PubMed=17686877; DOI=10.1128/JVI.00807-07;
RA   Li T.-C., Scotti P.D., Miyamura T., Takeda N.;
RT   "Latent infection of a new alphanodavirus in an insect cell line.";
RL   J. Virol. 81:10890-10896(2007).
RX   PubMed=20300881; DOI=10.1007/s12033-010-9268-3;
RA   Krammer F., Schinko T., Palmberger D., Tauer C., Messner P.,
RA   Grabherr R.;
RT   "Trichoplusia ni cells (High Five) are highly efficient for the
RT   production of influenza A virus-like particles: a comparison of two
RT   insect cell lines as production platforms for influenza vaccines.";
RL   Mol. Biotechnol. 45:226-234(2010).
RX   PubMed=21667340; DOI=10.1007/s12250-011-3177-x;
RA   Wu Y.-L., Jiang L., Hashimoto Y., Granados R.R., Li G.-X.;
RT   "Establishment, growth kinetics, and susceptibility to AcMNPV of heat
RT   tolerant lepidopteran cell lines.";
RL   Virol. Sin. 26:198-205(2011).
RX   PubMed=23848607; DOI=10.1021/pr400329k;
RA   An Y.-M., Rininger J.A., Jarvis D.L., Jing X.-H., Ye Z.-P., Aumiller J.J.,
RA   Eichelberger M.C., Cipollo J.F.;
RT   "Comparative glycomics analysis of influenza Hemagglutinin (H5N1)
RT   produced in vaccine relevant cell platforms.";
RL   J. Proteome Res. 12:3707-3720(2013).
RX   PubMed=24375231; DOI=10.1007/s10529-013-1429-6;
RA   Wilde M., Klausberger M., Palmberger D., Ernst W., Grabherr R.;
RT   "Tnao38, high five and Sf9 -- evaluation of host-virus interactions in
RT   three different insect cell lines: baculovirus production and
RT   recombinant protein expression.";
RL   Biotechnol. Lett. 36:743-749(2014).
RX   PubMed=29133148; DOI=10.1016/j.pep.2017.11.002;
RA   Geisler C., Jarvis D.L.;
RT   "Adventitious viruses in insect cell lines used for recombinant
RT   protein expression.";
RL   Protein Expr. Purif. 144:25-32(2017).
RX   PubMed=29376823; DOI=10.7554/eLife.31628;
RA   Fu Y., Yang Y.-J., Zhang H., Farley G., Wang J.-L., Quarles K.A.,
RA   Weng Z.-P., Zamore P.D.;
RT   "The genome of the Hi5 germ cell line from Trichoplusia ni, an
RT   agricultural pest and novel model for small RNA biology.";
RL   eLife 7:e31628.1-e31628.41(2018).
RX   PubMed=30449074; DOI=10.1111/1755-0998.12966;
RA   Chen W.-B., Yang X.-W., Tetreau G., Song X.-Z., Coutu C., Hegedus D.,
RA   Blissard G.W., Fei Z.-J., Wang P.;
RT   "A high-quality chromosome-level genome assembly of a generalist
RT   herbivore, Trichoplusia ni.";
RL   Mol. Ecol. Resour. 19:485-496(2019).