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Cellosaurus TIME.219 (CVCL_B6YR)

[Text version]
Cell line name TIME.219
Accession CVCL_B6YR
Resource Identification Initiative To cite this cell line use: TIME.219 (RRID:CVCL_B6YR)
Comments Characteristics: rKSHV.219 includes a RFP-GFP-Puro construct that contains RFP under the control of the lytic PAN RNA promoter, GFP under the control of human EF1a promoter and PuroR expressed by the RSV promoter.
Virology: Infected with a recombinant human herpesvirus 8 (HHV-8/KSHV) rKSHV.219.
Transfected with: HGNC; 11730; TERT.
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Transfected with: UniProtKB; Q9U6Y8; Discosoma sp. red fluorescent protein drFP583 (DsRed, RFP).
Transfected with: UniProtKB; P33967; B.cereus Bsr.
Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Foreskin, skin, dermis, microvascular endothelium; UBERON=UBERON_0001471+UBERON_0008339.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0047 (TIME)
Children:
CVCL_B6YS (iTIME.219)
Sex of cell Male
Age at sampling <1M
Category Telomerase immortalized cell line
STR profile Source(s): PubMed=33937098

Markers:
AmelogeninX,Y
CSF1PO11,12
D5S81811
D7S8208,9
D13S3179,11
D16S5399,12
TH016,7
TPOX8
vWA16,18

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Publications

PubMed=33937098; DOI=10.3389/fcimb.2021.654396
Dollery S.J., Maldonado T.D., Brenner E.A., Berger E.A.
iTIME.219: an immortalized KSHV infected endothelial cell line inducible by a KSHV-specific stimulus to transition from latency to lytic replication and infectious virus release.
Front. Cell. Infect. Microbiol. 11:654396.1-654396.9(2021)

Cross-references
Encyclopedic resources Wikidata; Q112041790
Entry history
Entry creation17-Mar-2022
Last entry update05-Oct-2023
Version number6