Cellosaurus logo
expasy logo

Cellosaurus TIME.219 (CVCL_B6YR)

[Text][XML][JSON]
Cell line name TIME.219
Accession CVCL_B6YR
Resource Identification Initiative To cite this cell line use: TIME.219 (RRID:CVCL_B6YR)
Comments Characteristics: rKSHV.219 includes a RFP-GFP-PuroR construct that contains RFP under the control of the lytic PAN RNA promoter, GFP under the control of human EF1a promoter and PuroR expressed by the RSV promoter.
Virology: Infected with a recombinant human herpesvirus 8 (HHV-8/KSHV) rKSHV.219.
Genetic integration: Method=Transduction; Gene=FPbase; EGE3L; DsRed (Note=drFP583, red fluorescent protein from Discosoma sp.).
Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT.
Genetic integration: Method=Transduction; Gene=UniProtKB; P33967; Bacillus cereus Bsr.
Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Derived from site: In situ; Foreskin, skin, dermis, microvascular endothelium; UBERON=UBERON_0001471+UBERON_0008339.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0047 (TIME)
Children:
CVCL_B6YS (iTIME.219)
Sex of cell Male
Age at sampling <1M
Category Telomerase immortalized cell line
STR profile Source(s): PubMed=33937098

Markers:
AmelogeninX,Y
CSF1PO11,12
D5S81811
D7S8208,9
D13S3179,11
D16S5399,12
TH016,7
TPOX8
vWA16,18

Run an STR similarity search on this cell line
Publications

PubMed=33937098; DOI=10.3389/fcimb.2021.654396; PMCID=PMC8080876
Stephen J. Dollery, Tania D. Maldonado, Eric A. Brenner, Edward A. Berger;
iTIME.219: an immortalized KSHV infected endothelial cell line inducible by a KSHV-specific stimulus to transition from latency to lytic replication and infectious virus release.
Front. Cell. Infect. Microbiol. 11:654396.1-654396.9(2021)

Cross-references
Encyclopedic resources Wikidata; Q112041790
Entry history
Entry creation17-Mar-2022
Last entry update14-Aug-2025
Version number10