Cellosaurus iTIME.219 (CVCL_B6YS)
| Cell line name | iTIME.219 | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Accession | CVCL_B6YS | ||||||||||||||||||
| Resource Identification Initiative | To cite this cell line use: iTIME.219 (RRID:CVCL_B6YS) | ||||||||||||||||||
| Comments | Characteristics: rKSHV.219 includes a RFP-GFP-Puro construct that contains RFP under the control of the lytic PAN RNA promoter, GFP under the control of human EF1a promoter and PuroR expressed by the RSV promoter. Characteristics: Transduced with pRetroX-Tet-On that contains a reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16 and with RetroX-Tight-Hyg-RTA. Virology: Maintains latent rKSHV.219 as indicated by expression of constitutively expressed (eGFP) but not a lytic phase (RFP) reporter gene. When exposed to either sodium butyrate or doxycycline, the cells are activated to lytic replication and express RFP and KSHV lytic genes and release large quantities of infectious virus (PubMed=33937098). Virology: Infected with a recombinant human herpesvirus 8 (HHV-8/KSHV) rKSHV.219. Transfected with: HGNC; 11730; TERT. Transfected with: UniProtKB; Q9U6Y8; Discosoma sp. red fluorescent protein drFP583 (DsRed, RFP). Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP). Transfected with: UniProtKB; P33967; B.cereus Bsr. Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR. Transfected with: UniProtKB; P00552; Transposon Tn5 neo. Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR). Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del). Derived from site: In situ; Foreskin, skin, dermis, microvascular endothelium; UBERON=UBERON_0001471+UBERON_0008339. | ||||||||||||||||||
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) | ||||||||||||||||||
| Hierarchy | Parent: CVCL_B6YR (TIME.219) | ||||||||||||||||||
| Sex of cell | Male | ||||||||||||||||||
| Age at sampling | <1M | ||||||||||||||||||
| Category | Telomerase immortalized cell line | ||||||||||||||||||
| STR profile | Source(s): PubMed=33937098 Markers:
Run an STR similarity search on this cell line | ||||||||||||||||||
| Publications | PubMed=33937098; DOI=10.3389/fcimb.2021.654396 | ||||||||||||||||||
| Cross-references | |||||||||||||||||||
| Encyclopedic resources | Wikidata; Q111733525 | ||||||||||||||||||
| Entry history | |||||||||||||||||||
| Entry creation | 17-Mar-2022 | ||||||||||||||||||
| Last entry update | 05-Oct-2023 | ||||||||||||||||||
| Version number | 6 | ||||||||||||||||||