ID   iTIME.219
AC   CVCL_B6YS
DR   Wikidata; Q111733525
RX   PubMed=33937098;
CC   Characteristics: rKSHV.219 includes a RFP-GFP-Puro construct that contains RFP under the control of the lytic PAN RNA promoter, GFP under the control of human EF1a promoter and PuroR expressed by the RSV promoter.
CC   Characteristics: Transduced with pRetroX-Tet-On that contains a reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16 and with RetroX-Tight-Hyg-RTA.
CC   Virology: Maintains latent rKSHV.219 as indicated by expression of constitutively expressed (eGFP) but not a lytic phase (RFP) reporter gene. When exposed to either sodium butyrate or doxycycline, the cells are activated to lytic replication and express RFP and KSHV lytic genes and release large quantities of infectious virus (PubMed=33937098).
CC   Virology: Infected with a recombinant human herpesvirus 8 (HHV-8/KSHV) rKSHV.219.
CC   Genetic integration: Method=Transduction; Gene=FPbase; EGE3L; DsRed (Note=drFP583, red fluorescent protein from Discosoma sp.).
CC   Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P33967; B.cereus Bsr.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Derived from site: In situ; Foreskin, skin, dermis, microvascular endothelium; UBERON=UBERON_0001471+UBERON_0008339.
ST   Source(s): PubMed=33937098
ST   Amelogenin: X,Y
ST   CSF1PO: 11,12
ST   D13S317: 9,11
ST   D16S539: 9,12
ST   D5S818: 11
ST   D7S820: 8,9
ST   TH01: 6,7
ST   TPOX: 8
ST   vWA: 16,18
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_B6YR ! TIME.219
SX   Male
AG   <1M
CA   Telomerase immortalized cell line
DT   Created: 17-03-22; Last updated: 10-04-25; Version: 9
//
RX   PubMed=33937098; DOI=10.3389/fcimb.2021.654396; PMCID=PMC8080876;
RA   Dollery S.J., Maldonado T.D., Brenner E.A., Berger E.A.;
RT   "iTIME.219: an immortalized KSHV infected endothelial cell line
RT   inducible by a KSHV-specific stimulus to transition from latency to
RT   lytic replication and infectious virus release.";
RL   Front. Cell. Infect. Microbiol. 11:654396.1-654396.9(2021).
//