ID   TIME.219
AC   CVCL_B6YR
DR   Wikidata; Q112041790
RX   PubMed=33937098;
CC   Characteristics: rKSHV.219 includes a RFP-GFP-Puro construct that contains RFP under the control of the lytic PAN RNA promoter, GFP under the control of human EF1a promoter and PuroR expressed by the RSV promoter.
CC   Virology: Infected with a recombinant human herpesvirus 8 (HHV-8/KSHV) rKSHV.219.
CC   Genetic integration: Method=Transduction; Gene=FPbase; EGE3L; DsRed (Note=drFP583, red fluorescent protein from Discosoma sp.).
CC   Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P33967; B.cereus Bsr.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: In situ; Foreskin, skin, dermis, microvascular endothelium; UBERON=UBERON_0001471+UBERON_0008339.
ST   Source(s): PubMed=33937098
ST   Amelogenin: X,Y
ST   CSF1PO: 11,12
ST   D13S317: 9,11
ST   D16S539: 9,12
ST   D5S818: 11
ST   D7S820: 8,9
ST   TH01: 6,7
ST   TPOX: 8
ST   vWA: 16,18
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0047 ! TIME
SX   Male
AG   <1M
CA   Telomerase immortalized cell line
DT   Created: 17-03-22; Last updated: 10-04-25; Version: 9
//
RX   PubMed=33937098; DOI=10.3389/fcimb.2021.654396; PMCID=PMC8080876;
RA   Dollery S.J., Maldonado T.D., Brenner E.A., Berger E.A.;
RT   "iTIME.219: an immortalized KSHV infected endothelial cell line
RT   inducible by a KSHV-specific stimulus to transition from latency to
RT   lytic replication and infectious virus release.";
RL   Front. Cell. Infect. Microbiol. 11:654396.1-654396.9(2021).
//