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Cellosaurus MC-38 (CVCL_B288)

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Cell line name MC-38
Synonyms MCA-38; MCA 38; MCA38; MC38; MC-38 B/6; Mouse Colon 38; Murine Carcinoma-38; Colon-38; Colon 38; Colon38; C38
Accession CVCL_B288
Resource Identification Initiative To cite this cell line use: MC-38 (RRID:CVCL_B288)
Comments Doubling time: 24.3 hours (PubMed=16320114).
Transformant: ChEBI; CHEBI_73755; 1,2-dimethylhydrazine (DMH).
Omics: Genomics; Mitochondrial genome sequencing.
Omics: Genomics; Whole exome sequencing.
Omics: Proteomics; HLA class I peptidome.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; SNP array analysis.
Miscellaneous: Comparative analysis of MC38 cells obtained from Kerafast (MC38-K) and from Leiden University Medical Center (MC38-L) shows major differences in term of mutations and mRNA expression (PubMed=36969213).
Caution: The authors of PubMed=25434994 indicate that they have sequenced the mitochondrial genome of the 'rat' cell line MCA38. Their submitted DNA sequence (KM820832.1) is indeed a rat sequence but is labelled as originating from 'rat isolate 406'.
Derived from site: In situ; Colon; UBERON=UBERON_0001155.
Disease Mouse colon adenocarcinoma (NCIt: C120044)
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: C57BL/6.
Hierarchy Children:
CVCL_F5BN (EDITGENE Il4i1 Knockout MC-38 Cell Line)CVCL_F6BE (EDITGENE MC-38-CopGFP)CVCL_F5TS (EDITGENE MC-38-FLUC)
CVCL_F5PU (EDITGENE MC38-Cas9)CVCL_E6WA (Genomeditech MC-38 H_AMHR2)CVCL_E6WB (Genomeditech MC-38 H_CD24)
CVCL_E6WC (Genomeditech MC-38 H_CD274(PD-L1))CVCL_E6WD (Genomeditech MC-38 H_CD47)CVCL_E6WE (Genomeditech MC-38 H_CEACAM5(CEA))
CVCL_E6WF (Genomeditech MC-38 H_CLDN18(isoform 2))CVCL_E6WG (Genomeditech MC-38 H_ERBB2(HER2))CVCL_E6WH (Genomeditech MC-38 H_ERBB2(HER2)+ERBB3(HER3))
CVCL_E6WI (Genomeditech MC-38 H_ERBB3(HER3))CVCL_E6WJ (Genomeditech MC-38 H_GPRC5D)CVCL_E6WK (Genomeditech MC-38 H_HLA-G+B2M)
CVCL_E6WL (Genomeditech MC-38 H_SIGLEC15+TYROBP)CVCL_F4L5 (HyCyte CT26 mKRAS_p.G12C_c.577G＞T cell line)CVCL_E1LY (HyCyte MC-38 KO-mCasp8)
CVCL_E1LZ (HyCyte MC-38 KO-mCd274)CVCL_D6C1 (HyCyte MC-38 KO-mCxcl9)CVCL_E1M0 (HyCyte MC-38 KO-mFmr1)
CVCL_D6C2 (HyCyte MC-38 KO-mMdm4)CVCL_E1M1 (HyCyte MC-38 KO-mPrmt3)CVCL_F4JL (HyCyte MC-38 KO-mRnf138)
CVCL_D6C3 (HyCyte MC-38 KO-mStk11)CVCL_F1RH (HyCyte MC-38 KO-mTgm2)CVCL_F4KC (HyCyte MC-38 mKras_p.G12D_c.35G>A cell line)
CVCL_F4V2 (HyCyte MC-38 stable cell line for mBCMA overexpression)CVCL_F4QH (HyCyte MC-38 stable cell line for mSlc5a5 overexpression)CVCL_E3HX (HyCyte MC-38-Luc)
CVCL_D0P4 (MC-38 B2m-KO)CVCL_5I36 (MC-38-CEA-1)CVCL_5I37 (MC-38-CEA-2)
CVCL_5I38 (MC-38-MUC-1)CVCL_E8T9 (MC38 B2m KO clone 1B2)CVCL_E8TA (MC38 B2m KO clone 1B3)
CVCL_XZ58 (MC38 human PDL1)CVCL_E8H1 (MC38-hCD39-LZ)CVCL_C8VZ (MC38-Luc)
CVCL_B7NQ (MC38-Luc-RFP)CVCL_XJ96 (MC38-OVA)CVCL_B7NM (MC38-PD-L1 KO)
CVCL_0A68 (MC38/0)CVCL_0A67 (MC38Luc1)CVCL_B9CM (MC38Luc4)
CVCL_B9CN (MC38Luc8)CVCL_D7FA (SL4)CVCL_E0XJ (Ubigene MC-38 Alkbh1 KO)
CVCL_E0XK (Ubigene MC-38 Arid1a KO)CVCL_E0XL (Ubigene MC-38 Arid2 KO)CVCL_E0XM (Ubigene MC-38 Atrx KO)
CVCL_E0XN (Ubigene MC-38 B2m KO)CVCL_E0XP (Ubigene MC-38 Cd274 KO)CVCL_E0XQ (Ubigene MC-38 Cd276 KO)
CVCL_E0XR (Ubigene MC-38 Cd38 KO)CVCL_E0XS (Ubigene MC-38 Cd47 KO)CVCL_E0XT (Ubigene MC-38 Epha2 KO)
CVCL_E0XU (Ubigene MC-38 Erbb2 KO)CVCL_E0XW (Ubigene MC-38 Fat1 KO)CVCL_E0XX (Ubigene MC-38 Il1r1 KO)
CVCL_E0XY (Ubigene MC-38 Kmt2c KO)CVCL_E0XZ (Ubigene MC-38 Kmt2d KO)CVCL_E0Y0 (Ubigene MC-38 Lrpprc KO)
CVCL_E0Y1 (Ubigene MC-38 Mif KO)CVCL_E0Y2 (Ubigene MC-38 Rb1 KO)CVCL_E0Y3 (Ubigene MC-38 Siglec15 KO)
CVCL_E0Y4 (Ubigene MC-38 Tnfrsf10b KO)CVCL_E0Y5 (Ubigene MC-38 Tnfrsf14 KO)CVCL_E0Y6 (Ubigene MC-38 Tnfrsf17 KO)
CVCL_E0Y7 (Ubigene MC-38 Tnfrsf1b KO)CVCL_E0Y8 (Ubigene MC-38 Ythdf1 KO)
Sex of cell Female
Age at sampling Age unspecified
Category Cancer cell line
STR profile Source(s): Millipore=SCC172; Ubigene=YC-A002

Markers:
Mouse STR 1-115,16,17
Mouse STR 1-218,19,20
Mouse STR 2-116
Mouse STR 3-214,15
Mouse STR 4-220.3
Mouse STR 5-516,17 (Ubigene=YC-A002)
17 (Millipore=SCC172)
Mouse STR 6-417,18 (Millipore=SCC172)
18 (Ubigene=YC-A002)
Mouse STR 6-715,16
Mouse STR 7-126.2
Mouse STR 8-116
Mouse STR 11-216
Mouse STR 12-116,17,18 (Millipore=SCC172)
17 (Ubigene=YC-A002)
Mouse STR 13-117
Mouse STR 15-322.3 (Millipore=SCC172)
22.3,23.3 (Ubigene=YC-A002)
Mouse STR 17-215,16
Mouse STR 18-315,16
Mouse STR 19-213
Mouse STR X-127

Run an STR similarity search on this cell line
Web pages Provider; Altogen; Xenograft model; https://altogenlabs.com/xenograft-models/colon-cancer-xenograft/mc38-xenograft-model/
Publications

PubMed=1149045
Thomas H. Corbett, Daniel Pratt Griswold Jr., Billy J. Roberts, John C. Peckham, Frank M. Schabel Jr.;
Tumor induction relationships in development of transplantable cancers of the colon in mice for chemotherapy assays, with a note on carcinogen structure.
Cancer Res. 35:2434-2439(1975)

PubMed=1255804; DOI=10.1093/jnci/56.4.871
Mong H. Tan, Edward Douglas Holyoke, Martin H. Goldrosen;
Murine colon adenocarcinomas: methods for selective culture in vitro.
J. Natl. Cancer Inst. 56:871-873(1976)

PubMed=16320114; DOI=10.1007/s10585-005-3585-0
Megumi Morimoto-Tomita, Yoshimi Ohashi, Azusa Matsubara, Makoto Tsuiji, Tatsuro Irimura;
Mouse colon carcinoma cells established for high incidence of experimental hepatic metastasis exhibit accelerated and anchorage-independent growth.
Clin. Exp. Metastasis 22:513-521(2005)

PubMed=25277546; DOI=10.1186/1471-2164-15-847; PMCID=PMC4198738
John Paul Didion, Ryan John Buus, Zohreh Naghashfar, David W. Threadgill, Herbert Carpenter Morse 3rd, Fernando Pardo-Manuel de Villena;
SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.
BMC Genomics 15:847.1-847.11(2014)

PubMed=25428506; DOI=10.1038/nature14001
Mahesh Yadav, Suchit Jhunjhunwala, Qui T. Phung, Patrick J. Lupardus, Joshua Tanguay, Stephanie M. Bumbaca, Christian Franci, Tommy K. Cheung, Jens Fritsche, Toni Weinschenk, Zora Modrusan ...Show all 14 authors... , Ira Mellman, Jennie R. Lill, Lelia Delamarre; Show fewer authors
Predicting immunogenic tumour mutations by combining mass spectrometry and exome sequencing.
Nature 515:572-576(2014)

PubMed=25434994; DOI=10.3109/19401736.2014.974176
Chun-Fen Zhang, Xiao-Jie Yu, Bao-Yong Wang, Hong-Wei Chen, Cheng-En Hu;
Whole mitochondrial genome sequence of a rat colorectal cancer MCA38 cell line.
Mitochondrial DNA A. DNA Mapp. Seq. Anal. 27:2034-2035(2016)

PubMed=36969213; DOI=10.3389/fimmu.2023.1102282; PMCID=PMC10030996
Barbara Schrors, Brett J. Hos, Ikra Gizem Yildiz, Martin Lower, Franziska Lang, Christoph Holtstrater, Julia Becker, Mathias Vormehr, Ugur Sahin, Ferdinand Antonius Ossendorp, Mustafa Diken;
MC38 colorectal tumor cell lines from two different sources display substantial differences in transcriptome, mutanome and neoantigen expression.
Front. Immunol. 14:1102282.1-1102282.10(2023)

Cross-references
Cell line collections (Providers) ABM; T8291
CLS; 305223 - Discontinued
EDITGENE; EDC00075
Hysigen; TCM-C790
Kerafast; ENH204-FP
Millipore; SCC172
NCI-DCTD; Colon 38
Ubigene; YC-A002
Cell line databases/resources CCRID; 1101MOU-PUMC000523
Lonza; 893
Anatomy/cell type resources BTO; BTO_0004163
Chemistry resources ChEMBL-Cells; CHEMBL3307696
ChEMBL-Targets; CHEMBL612821
PubChem_Cell_line; CVCL_B288
Encyclopedic resources Wikidata; Q54904168
Entry history
Entry creation06-Jun-2012
Last entry update25-Jun-2026
Version number27