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Cellosaurus L8 (CVCL_4236)

[Text version]
Cell line name L8
Synonyms L-8
Accession CVCL_4236
Resource Identification Initiative To cite this cell line use: L8 (RRID:CVCL_4236)
Comments Group: Space-flown cell line (cellonaut).
Characteristics: Upon becoming confluent, will fuse to form cross striated multinucleated muscle fibers (ATCC=CRL-1769).
Doubling time: 19.1 +- 2.7 hours (Note=In 10% serum), 25.1 +- 3.5 hours (Note=In 1% serum) (PubMed=269441).
Anecdotal: Have been flown in space on shuttle flight STS-45 to study growth in microgravity (PubMed=7962182).
Derived from site: In situ; Skeletal muscle; UBERON=UBERON_0001134.
Cell type: Myoblast; CL=CL_0000056.
Species of origin Rattus norvegicus (Rat) (NCBI Taxonomy: 10116)
Breed/subspecies: Wistar.
Hierarchy Children:
CVCL_0252 (L8 E63)CVCL_C4KT (L8 fu-1)CVCL_C4L2 (L8 fu-13)
CVCL_C4L3 (L8 fu-14)CVCL_C4L4 (L8 fu-15)CVCL_C4L5 (L8 fu-16)
CVCL_C4KU (L8 fu-2)CVCL_C4KV (L8 fu-3)CVCL_C4KW (L8 fu-4)
CVCL_C4KX (L8 fu-5)CVCL_C4KY (L8 fu-6)CVCL_C4KZ (L8 fu-7)
CVCL_C4L0 (L8 fu-8)CVCL_C4L1 (L8 fu-9)CVCL_C571 (L84)
Sex of cell Sex unspecified
Age at sampling <1D
Category Spontaneously immortalized cell line
Web pages https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/l/cell-lines-detail-341.html

PubMed=5481965; DOI=10.1016/S0012-1606(70)80004-5
Richler C., Yaffe D.
The in vitro cultivation and differentiation capacities of myogenic cell lines.
Dev. Biol. 23:1-22(1970)

PubMed=269441; DOI=10.1073/pnas.74.9.3888
Kaufman S.J., Parks C.M.
Loss of growth control and differentiation in the fu-1 variant of the L8 line of rat myoblasts.
Proc. Natl. Acad. Sci. U.S.A. 74:3888-3892(1977)

PubMed=558123; DOI=10.1111/j.1432-0436.1977.tb01507.x
Yaffe D., Saxel O.
A myogenic cell line with altered serum requirements for differentiation.
Differentiation 7:159-166(1977)

PubMed=6243570; DOI=10.1016/0014-4827(80)90128-7
Kaufman S.J., Parks C.M., Bohn J., Faiman L.E.
Transformation is an alternative to normal skeletal muscle development.
Exp. Cell Res. 125:333-349(1980)

PubMed=7962182; DOI=10.1002/jcb.240550412
Kulesh D.A., Anderson L.H., Wilson B., Otis E.J., Elgin D.M., Barker M.J., Mehm W.J., Kearney G.P.
Space shuttle flight (STS-45) of L8 myoblast cells results in the isolation of a nonfusing cell line variant.
J. Cell. Biochem. 55:530-544(1994)

Cell line collections (Providers) ATCC; CRL-1769
ECACC; 95102434
Cell line databases/resources CLO; CLO_0007213
CLDB; cl3148
Anatomy/cell type resources BTO; BTO:0002810
Encyclopedic resources Wikidata; Q54901838
Entry history
Entry creation04-Apr-2012
Last entry update29-Jun-2023
Version number16