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Cellosaurus L8 [Rat muscle] (CVCL_4236)

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Cell line name L8 [Rat muscle]
Synonyms L-8
Accession CVCL_4236
Resource Identification Initiative To cite this cell line use: L8 [Rat muscle] (RRID:CVCL_4236)
Comments Group: Space-flown cell line (cellonaut).
Characteristics: Upon becoming confluent, will fuse to form cross striated multinucleated muscle fibers (ATCC=CRL-1769).
Doubling time: 19.1 +- 2.7 hours (Note=In 10% serum), 25.1 +- 3.5 hours (Note=In 1% serum) (PubMed=269441).
Anecdotal: Have been flown in space on shuttle flight STS-45 to study growth in microgravity (PubMed=7962182).
Derived from site: In situ; Skeletal muscle; UBERON=UBERON_0001134.
Cell type: Myoblast; CL=CL_0000056.
Species of origin Rattus norvegicus (Rat) (NCBI Taxonomy: 10116)
Breed/subspecies: Wistar; RS=RS_0000955.
Hierarchy Children:
CVCL_0252 (L8 E63)CVCL_C4KT (L8 fu-1)CVCL_C4L2 (L8 fu-13)
CVCL_C4L3 (L8 fu-14)CVCL_C4L4 (L8 fu-15)CVCL_C4L5 (L8 fu-16)
CVCL_C4KU (L8 fu-2)CVCL_C4KV (L8 fu-3)CVCL_C4KW (L8 fu-4)
CVCL_C4KX (L8 fu-5)CVCL_C4KY (L8 fu-6)CVCL_C4KZ (L8 fu-7)
CVCL_C4L0 (L8 fu-8)CVCL_C4L1 (L8 fu-9)CVCL_C571 (L84)
CVCL_WN49 (L8SF)
Sex of cell Sex unspecified
Age at sampling <1D
Category Spontaneously immortalized cell line
Web pages Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/l/cell-lines-detail-341.html
Publications

PubMed=5481965; DOI=10.1016/S0012-1606(70)80004-5
Carmelit Richler, David Yaffe;
The in vitro cultivation and differentiation capacities of myogenic cell lines.
Dev. Biol. 23:1-22(1970)

PubMed=269441; DOI=10.1073/pnas.74.9.3888; PMCID=PMC431773
Stephen J. Kaufman, Christine M. Parks;
Loss of growth control and differentiation in the fu-1 variant of the L8 line of rat myoblasts.
Proc. Natl. Acad. Sci. U.S.A. 74:3888-3892(1977)

PubMed=558123; DOI=10.1111/j.1432-0436.1977.tb01507.x
David Yaffe, Ora Saxel;
A myogenic cell line with altered serum requirements for differentiation.
Differentiation 7:159-166(1977)

PubMed=6243570; DOI=10.1016/0014-4827(80)90128-7
Stephen J. Kaufman, Christine M. Parks, Joseph Bohn, Lia E. Faiman;
Transformation is an alternative to normal skeletal muscle development.
Exp. Cell Res. 125:333-349(1980)

PubMed=7962182; DOI=10.1002/jcb.240550412
David A. Kulesh, Loraine H. Anderson, Bernard Wilson, Ericka J. Otis, Diane M. Elgin, Michael J. Barker, William J. Mehm, George P. Kearney;
Space shuttle flight (STS-45) of L8 myoblast cells results in the isolation of a nonfusing cell line variant.
J. Cell. Biochem. 55:530-544(1994)

Cross-references
Cell line collections (Providers) ATCC; CRL-1769
ECACC; 95102434
IZSLER; BS CL 172
Cell line databases/resources CLO; CLO_0007213
CLDB; cl3148
Anatomy/cell type resources BTO; BTO_0002810
Encyclopedic resources Wikidata; Q54901838
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number19