ID   L8
AC   CVCL_4236
SY   L-8
DR   BTO; BTO:0002810
DR   CLO; CLO_0007213
DR   CLDB; cl3148
DR   ATCC; CRL-1769
DR   ECACC; 95102434
DR   IZSLER; BS CL 172
DR   Wikidata; Q54901838
RX   PubMed=269441;
RX   PubMed=558123;
RX   PubMed=5481965;
RX   PubMed=6243570;
RX   PubMed=7962182;
WW   https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/l/cell-lines-detail-341.html
CC   Group: Space-flown cell line (cellonaut).
CC   Characteristics: Upon becoming confluent, will fuse to form cross striated multinucleated muscle fibers (ATCC=CRL-1769).
CC   Doubling time: 19.1 +- 2.7 hours (Note=In 10% serum), 25.1 +- 3.5 hours (Note=In 1% serum) (PubMed=269441).
CC   Anecdotal: Have been flown in space on shuttle flight STS-45 to study growth in microgravity (PubMed=7962182).
CC   Derived from site: In situ; Skeletal muscle; UBERON=UBERON_0001134.
CC   Cell type: Myoblast; CL=CL_0000056.
CC   Breed/subspecies: Wistar.
OX   NCBI_TaxID=10116; ! Rattus norvegicus (Rat)
SX   Sex unspecified
AG   <1D
CA   Spontaneously immortalized cell line
DT   Created: 04-04-12; Last updated: 29-06-23; Version: 16
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RX   PubMed=269441; DOI=10.1073/pnas.74.9.3888;
RA   Kaufman S.J., Parks C.M.;
RT   "Loss of growth control and differentiation in the fu-1 variant of the
RT   L8 line of rat myoblasts.";
RL   Proc. Natl. Acad. Sci. U.S.A. 74:3888-3892(1977).
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RX   PubMed=558123; DOI=10.1111/j.1432-0436.1977.tb01507.x;
RA   Yaffe D., Saxel O.;
RT   "A myogenic cell line with altered serum requirements for
RT   differentiation.";
RL   Differentiation 7:159-166(1977).
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RX   PubMed=5481965; DOI=10.1016/S0012-1606(70)80004-5;
RA   Richler C., Yaffe D.;
RT   "The in vitro cultivation and differentiation capacities of myogenic
RT   cell lines.";
RL   Dev. Biol. 23:1-22(1970).
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RX   PubMed=6243570; DOI=10.1016/0014-4827(80)90128-7;
RA   Kaufman S.J., Parks C.M., Bohn J., Faiman L.E.;
RT   "Transformation is an alternative to normal skeletal muscle
RT   development.";
RL   Exp. Cell Res. 125:333-349(1980).
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RX   PubMed=7962182; DOI=10.1002/jcb.240550412;
RA   Kulesh D.A., Anderson L.H., Wilson B., Otis E.J., Elgin D.M.,
RA   Barker M.J., Mehm W.J., Kearney G.P.;
RT   "Space shuttle flight (STS-45) of L8 myoblast cells results in the
RT   isolation of a nonfusing cell line variant.";
RL   J. Cell. Biochem. 55:530-544(1994).
//