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Cellosaurus IPEC-J2 (CVCL_2246)

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Cell line name IPEC-J2
Synonyms IPECJ2; Intestinal Porcine Epithelial Cell line-J2
Accession CVCL_2246
Resource Identification Initiative To cite this cell line use: IPEC-J2 (RRID:CVCL_2246)
Comments Characteristics: Undergoes a process of spontaneous differentiation that leads to the formation of a polarized monolayer with low or high transepithelial electrical resistance (TEER), depending on the type of serum added to the culture medium, within 1-2 weeks (PubMed=29787056).
Virology: Susceptible to infection by porcine circovirus 2 (PCV2) (PubMed=25407967).
Doubling time: ~40-60 hours (DSMZ=ACC-701).
Omics: Proteomics.
Omics: Transcriptomics; Microarray.
Derived from site: In situ; Small intestine, jejunum; UBERON=UBERON_0002115.
Cell type: Enterocyte; CL=CL_0000584.
Species of origin Sus scrofa (Pig) (NCBI Taxonomy: 9823)
Hierarchy Children:
CVCL_6C41 (IPEC-J2 MDR1)CVCL_A9M7 (IPEC-J2 rMDR1a)CVCL_6C22 (IPEC-J2-3)
CVCL_6C23 (IPEC-J2-9)CVCL_C8W6 (IPEC-J2-EGFP)CVCL_ZZ35 (SD-PJEC)
CVCL_E0WB (Ubigene IPEC-J2 ANPEP KO)CVCL_E0WC (Ubigene IPEC-J2 GDPD2 KO)CVCL_E0WD (Ubigene IPEC-J2 KLF4 KO)
CVCL_E0WE (Ubigene IPEC-J2 YTHDF2 KO)
Sex of cell Sex unspecified
Age at sampling <1D
Category Spontaneously immortalized cell line
Publications

DOI=10.1016/S0016-5085(89)80002-2
Helen M. Berschneider;
Development of normal cultured small intestinal epithelial cell lines which transport Na and Cl.
Gastroenterology 96:A41-A41(1989)

PubMed=16215741; DOI=10.1007/s00418-005-0067-z
Peter Schierack, Marcel Nordhoff, Marion Pollmann, Karl Dietrich Weyrauch, Salah Amasheh, Ulrike Lodemann, Jorg Jores, Babila Tachu, Sylvia Kleta, Anthony T. Blikslager, Karsten Tedin, Lothar H. Wieler;
Characterization of a porcine intestinal epithelial cell line for in vitro studies of microbial pathogenesis in swine.
Histochem. Cell Biol. 125:293-305(2006)

PubMed=21626283; DOI=10.1007/s10616-011-9362-9; PMCID=PMC3140839
Marisa M. Geens, Theo A. Niewold;
Optimizing culture conditions of a porcine epithelial cell line IPEC-J2 through a histological and physiological characterization.
Cytotechnology 63:415-423(2011)

PubMed=22074860; DOI=10.1016/j.vetmic.2011.10.017; PMCID=PMC3289732
Amanda J. Brosnahan, David R. Brown;
Porcine IPEC-J2 intestinal epithelial cells in microbiological investigations.
Vet. Microbiol. 156:229-237(2012)

PubMed=22193509; DOI=10.1007/s10616-011-9420-3; PMCID=PMC3397115
Klaus G. Steube, Anne-Leena Koelz, Cord C. Uphoff, Hans Gunther Drexler, Jeannette Kluess, Pablo Steinberg;
The necessity of identity assessment of animal intestinal cell lines: a case report.
Cytotechnology 64:373-378(2012)

DOI=10.1155/2013/980651
Ann Cathrine Findal Stoy, Peter M.H. Heegaard, Per T. Sangild, Mette V. Ostergaard, Kerstin Skovgaard;
Gene expression analysis of the IPEC-J2 cell line: a simple model for the inflammation-sensitive preterm intestine.
ISRN Genomics 2013:980651.1-980651.7(2013)

PubMed=24260272; DOI=10.1371/journal.pone.0079643; PMCID=PMC3829867
Silke S. Zakrzewski, Jan F. Richter, Susanne M. Krug, Britta Jebautzke, In-Fah M. Lee, Juliane Rieger, Monika Sachtleben, Angelika Bondzio, Jorg-Dieter Schulzke, Michael Fromm, Dorothee Gunzel;
Improved cell line IPEC-J2, characterized as a model for porcine jejunal epithelium.
PLoS ONE 8:e79643.1-e79643.12(2013)

PubMed=25407967; DOI=10.1186/s12985-014-0193-0; PMCID=PMC4237784
Meng-Fei Yan, Li-Qi Zhu, Qian Yang;
Infection of porcine circovirus 2 (PCV2) in intestinal porcine epithelial cell line (IPEC-J2) and interaction between PCV2 and IPEC-J2 microfilaments.
Virol. J. 11:193.1-193.9(2014)

PubMed=26147118; DOI=10.1371/journal.pone.0132323; PMCID=PMC4493080
Constanze Nossol, Aniko Barta-Boszormenyi, Stefan Kahlert, Werner Zuschratter, Heidi Faber-Zuschratter, Nicole Reinhardt, Siriluk Ponsuksili, Klaus Wimmers, Anne-Kathrin Diesing, Hermann-Josef Rothkotter;
Comparing two intestinal porcine epithelial cell lines (IPECs): morphological differentiation, function and metabolism.
PLoS ONE 10:e0132323.1-e0132323.20(2015)

PubMed=29787056; DOI=10.1007/978-3-319-16104-4_12
Hans Vergauwen;
The IPEC-J2 cell line.
(In book chapter) The impact of food bioactives on health. In vitro and ex vivo models; Verhoeckx, Kitty & Cotter, Paul David & Lopez-Exposito, Ivan & Kleiveland, Charlotte Ramstad & Lea, Tor & Mackie, Alan Robert & Requena, Teresa & Swiatecka, Dominika & Wichers, Harry J. (eds.); pp.125-134; Springer; Cham; Switzerland (2015)

PubMed=26651362; DOI=10.1021/acs.molpharmaceut.5b00874
Lasse Saaby, Hans Christian Cederberg Helms, Birger Brodin;
IPEC-J2 MDR1, a novel high-resistance cell line with functional expression of human P-glycoprotein (ABCB1) for drug screening studies.
Mol. Pharm. 13:640-652(2016)

PubMed=26975772; DOI=10.4014/jmb.1512.12075
Sang Hoon Kim, Edward Alain B. Pajarillo, Marilen P. Balolong, Ji Yoon Lee, Dae-Kyung Kang;
Constructing proteome reference map of the porcine jejunal cell line (IPEC-J2) by label-free mass spectrometry.
J. Microbiol. Biotechnol. 26:1124-1131(2016)

PubMed=32708885; DOI=10.3390/pharmaceutics12070673; PMCID=PMC7408396
Lasse Saaby, Josefine Trasborg, Mikkel Aabech Rasmussen, Bjorn Holst, Birger Brodin;
IPEC-J2 rMdr1a, a new cell line with functional expression of rat P-glycoprotein encoded by rat Mdr1a for drug screening purposes.
Pharmaceutics 12:673.1-673.17(2020)

Cross-references
Cell line collections (Providers) DSMZ; ACC-701
IZSLER; BS CL 205
Ubigene; YC-C072
Cell line databases/resources DSMZCellDive; ACC-701
Anatomy/cell type resources BTO; BTO_0006402
Encyclopedic resources Wikidata; Q54897969
Gene expression databases GEO; GSM1646398
GEO; GSM1646399
GEO; GSM1646400
Entry history
Entry creation04-Apr-2012
Last entry update14-Aug-2025
Version number21