AC   CVCL_2246
SY   IPECJ2; Intestinal Porcine Epithelial Cell line-J2
DR   BTO; BTO:0006402
DR   DSMZ; ACC-701
DR   DSMZCellDive; ACC-701
DR   GEO; GSM1646398
DR   GEO; GSM1646399
DR   GEO; GSM1646400
DR   Ubigene; YC-C072
DR   Wikidata; Q54897969
RX   DOI=10.1016/S0016-5085(89)80002-2;
RX   DOI=10.1155/2013/980651;
RX   PubMed=16215741;
RX   PubMed=21626283;
RX   PubMed=22074860;
RX   PubMed=22193509;
RX   PubMed=24260272;
RX   PubMed=25407967;
RX   PubMed=26147118;
RX   PubMed=26651362;
RX   PubMed=26975772;
RX   PubMed=29787056;
RX   PubMed=32708885;
CC   Characteristics: Undergoes a process of spontaneous differentiation that leads to the formation of a polarized monolayer with low or high transepithelial electrical resistance (TEER), depending on the type of serum added to the culture medium, within 1-2 weeks (PubMed=29787056).
CC   Virology: Susceptible to infection by porcine circovirus type 2 (PCV2) (PubMed=25407967).
CC   Doubling time: ~40-60 hours (DSMZ=ACC-701).
CC   Omics: Deep proteome analysis.
CC   Omics: Transcriptome analysis by microarray.
CC   Derived from site: In situ; Small intestine, jejunum; UBERON=UBERON_0002115.
CC   Cell type: Enterocyte; CL=CL_0000584.
OX   NCBI_TaxID=9823; ! Sus scrofa (Pig)
SX   Sex unspecified
AG   <1D
CA   Spontaneously immortalized cell line
DT   Created: 04-04-12; Last updated: 05-10-23; Version: 18
RX   DOI=10.1016/S0016-5085(89)80002-2;
RA   Berschneider H.M.;
RT   "Development of normal cultured small intestinal epithelial cell lines
RT   which transport Na and Cl.";
RL   Gastroenterology 96:A41-A41(1989).
RX   DOI=10.1155/2013/980651;
RA   Stoy A.C.F., Heegaard P.M.H., Sangild P.T., Ostergaard M.V.,
RA   Skovgaard K.;
RT   "Gene expression analysis of the IPEC-J2 cell line: a simple model for
RT   the inflammation-sensitive preterm intestine.";
RL   ISRN Genomics 2013:980651.1-980651.7(2013).
RX   PubMed=16215741; DOI=10.1007/s00418-005-0067-z;
RA   Schierack P., Nordhoff M., Pollmann M., Weyrauch K.D., Amasheh S.,
RA   Lodemann U., Jores J., Tachu B., Kleta S., Blikslager A., Tedin K.,
RA   Wieler L.H.;
RT   "Characterization of a porcine intestinal epithelial cell line for in
RT   vitro studies of microbial pathogenesis in swine.";
RL   Histochem. Cell Biol. 125:293-305(2006).
RX   PubMed=21626283; DOI=10.1007/s10616-011-9362-9;
RA   Geens M.M., Niewold T.A.;
RT   "Optimizing culture conditions of a porcine epithelial cell line
RT   IPEC-J2 through a histological and physiological characterization.";
RL   Cytotechnology 63:415-423(2011).
RX   PubMed=22074860; DOI=10.1016/j.vetmic.2011.10.017;
RA   Brosnahan A.J., Brown D.R.;
RT   "Porcine IPEC-J2 intestinal epithelial cells in microbiological
RT   investigations.";
RL   Vet. Microbiol. 156:229-237(2012).
RX   PubMed=22193509; DOI=10.1007/s10616-011-9420-3;
RA   Steube K.G., Koelz A.-L., Uphoff C.C., Drexler H.G., Kluess J.,
RA   Steinberg P.;
RT   "The necessity of identity assessment of animal intestinal cell lines:
RT   a case report.";
RL   Cytotechnology 64:373-378(2012).
RX   PubMed=24260272; DOI=10.1371/journal.pone.0079643;
RA   Zakrzewski S.S., Richter J.F., Krug S.M., Jebautzke B., Lee I.-F.M.,
RA   Rieger J., Sachtleben M., Bondzio A., Schulzke J.-D., Fromm M.,
RA   Gunzel D.;
RT   "Improved cell line IPEC-J2, characterized as a model for porcine
RT   jejunal epithelium.";
RL   PLoS ONE 8:E79643-E79643(2013).
RX   PubMed=25407967; DOI=10.1186/s12985-014-0193-0;
RA   Yan M.-F., Zhu L.-Q., Yang Q.;
RT   "Infection of porcine circovirus 2 (PCV2) in intestinal porcine
RT   epithelial cell line (IPEC-J2) and interaction between PCV2 and
RT   IPEC-J2 microfilaments.";
RL   Virol. J. 11:193.1-193.9(2014).
RX   PubMed=26147118; DOI=10.1371/journal.pone.0132323;
RA   Nossol C., Barta-Boszormenyi A., Kahlert S., Zuschratter W.,
RA   Faber-Zuschratter H., Reinhardt N., Ponsuksili S., Wimmers K.,
RA   Diesing A.-K., Rothkotter H.-J.;
RT   "Comparing two intestinal porcine epithelial cell lines (IPECs):
RT   morphological differentiation, function and metabolism.";
RL   PLoS ONE 10:E0132323-E0132323(2015).
RX   PubMed=26651362; DOI=10.1021/acs.molpharmaceut.5b00874;
RA   Saaby L., Helms H.C.C., Brodin B.;
RT   "IPEC-J2 MDR1, a novel high-resistance cell line with functional
RT   expression of human P-glycoprotein (ABCB1) for drug screening
RT   studies.";
RL   Mol. Pharm. 13:640-652(2016).
RX   PubMed=26975772; DOI=10.4014/jmb.1512.12075;
RA   Kim S.H., Pajarillo E.A.B., Balolong M.P., Lee J.Y., Kang D.-K.;
RT   "Constructing proteome reference map of the porcine jejunal cell line
RT   (IPEC-J2) by label-free mass spectrometry.";
RL   J. Microbiol. Biotechnol. 26:1124-1131(2016).
RX   PubMed=29787056; DOI=10.1007/978-3-319-16104-4_12;
RA   Vergauwen H.;
RT   "The IPEC-J2 cell line.";
RL   (In) The impact of food bioactives on health. In vitro and ex vivo models; Verhoeckx K., Cotter P., Lopez-Exposito I., Kleiveland C., Lea T., Mackie A., Requena T., Swiatecka D., Wichers H. (eds.); pp.125-134; Springer; Cham (2015).
RX   PubMed=32708885; DOI=10.3390/pharmaceutics12070673;
RA   Saaby L., Trasborg J., Rasmussen M.A., Holst B., Brodin B.;
RT   "IPEC-J2 rMdr1a, a new cell line with functional expression of rat
RT   P-glycoprotein encoded by rat Mdr1a for drug screening purposes.";
RL   Pharmaceutics 12:673.1-673.17(2020).