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Cellosaurus ECV-304 (CVCL_2029)

[Text version]
Cell line name ECV-304
Synonyms ECV 304; ECV304; ECV; E304; T24(ECV304)
Accession CVCL_2029
Resource Identification Initiative To cite this cell line use: ECV-304 (RRID:CVCL_2029)
Comments Problematic cell line: Contaminated. Shown to be a T24 derivative (DOI=10.11418/jtca1981.18.4_329; PubMed=10508494; PubMed=10614862; PubMed=12197766; PubMed=20143388). Originally thought to originate from endothelial cells obtained from the umbilical vein of a healthy donor.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00117.
Population: Caucasian; Swedish.
Virology: Not susceptible to infection by SARS coronavirus 2 (SARS-CoV-2) (COVID-19) (PubMed=33389257).
Doubling time: 35 hours (PubMed=1817644); ~2 days (DSMZ=ACC-310).
Omics: Proteomics; PTM; Tyrosine phosphorylation.
Miscellaneous: Formerly the CCRID database had 2 entries describing this cell line (3111C0002000000074, 3142C0001000000023). They were one of the sources for the STR profile of this entry.
Derived from site: In situ; Urinary bladder; UBERON=UBERON_0001255.
Sequence variations
  • Mutation; HGNC; HGNC:5173; HRAS; Simple; p.Gly12Val (c.35G>T); ClinVar=VCV000012600; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Tyr126Ter (c.378C>G); ClinVar=VCV000578988; Zygosity=Homozygous (from parent cell line).
Disease Bladder carcinoma (NCIt: C4912)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0554 (T24)
Children:
CVCL_KS70 (CellSensor IL-1-bla ECV304)CVCL_UY91 (t-HUE1)CVCL_J427 (t-HUE2)
Sex of cell Female
Age at sampling 82Y
Category Cancer cell line
STR profile Source(s): ATCC=CRL-1998; CCRID; CLS=300452; DSMZ=ACC-310; ECACC=92091712; JCRB=JCRB0744; PubMed=11416159

Markers:
AmelogeninX
CSF1PO12
D2S133820,23
D3S135816
D5S81810
D7S82010,11
D8S117910,14 (DSMZ=ACC-310)
14 (CLS=300452; PubMed=11416159)
D13S31712
D16S5399
D18S5116,18
D19S43313,14
D21S1129
FGA17,22
Penta D11,15
Penta E7,10
TH016
TPOX8,11
vWA17

Run an STR similarity search on this cell line
Web pages Info; ATCC; -; https://www.atcc.org/en/support/technical-support/faqs/ecv-304-atcc-crl-1998
Info; ICLAC; -; https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx
Info; JCRB; -; https://cellbank.nibiohn.go.jp/legacy/cellbank/qualitycontrol/identification/summary.htm
Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/e/cell-lines-detail-2.html
Publications

PubMed=1690702; DOI=10.1007/BF02624456
Kimiko Takahashi, Yoshio Sawasaki, Jun-ichi Hata, Kiyoshi Mukai, Tamotsu Goto;
Spontaneous transformation and immortalization of human endothelial cells.
In Vitro Cell. Dev. Biol. 26:265-274(1990)

PubMed=1817644
Mieko Kobayashi, Masaaki Kondo, Youji Mitsui;
Establishment of human endothelial cell lines in a serum-free culture and its application for expression of transfected prepro endothelin gene.
Hum. Cell 4:296-305(1991)

PubMed=1378828; DOI=10.1007/BF02634037
Kimiko Takahasi, Yoshio Sawasaki;
Rare spontaneously transformed human endothelial cell line provides useful research tool.
In Vitro Cell. Dev. Biol. Anim. 28:380-382(1992)

PubMed=9290701; DOI=10.1002/(SICI)1098-2744(199708)19:4<243::AID-MC5>3.0.CO;2-D
Li-Qun Jia, Motonobu Osada, Chikashi Ishioka, Makio Gamo, Shuntaro Ikawa, Takao Suzuki, Hideki Shimodaira, Tomohito Niitani, Toshio Kudo, Mitoshi Akiyama, Narimiti Kimura ...Show all 18 authors... , Mitsuyoshi Matsuo, Hiroshi Mizusawa, Noriho Tanaka, Hideki Koyama, Masayoshi Namba, Ryunosuke Kanamaru, Toshio Kuroki; Show fewer authors
Screening the p53 status of human cell lines using a yeast functional assay.
Mol. Carcinog. 19:243-253(1997)

DOI=10.11418/jtca1981.18.4_329
Hideyuki Tanabe, Yoko Takada, Daisuke Minegishi, Miharu Kurematsu, Tohru Masui, Hiroshi Mizusawa;
Cell line individualization by STR multiplex system in the cell bank found cross-contamination between ECV304 and EJ-1/T24.
Tissue Cult. Res. Commun. 18:329-338(1999)

PubMed=10508494; DOI=10.1002/(SICI)1097-0215(19991112)83:4<555::AID-IJC19>3.0.CO;2-2
Roderick A.F. MacLeod, Wilhelm Gerhard Dirks, Yoshinobu Matsuo, Maren Kaufmann, Herbert Milch, Hans Gunther Drexler;
Widespread intraspecies cross-contamination of human tumor cell lines arising at source.
Int. J. Cancer 83:555-563(1999)

PubMed=10614862; DOI=10.1007/s11626-999-0091-8
Wilhelm Gerhard Dirks, Roderick A.F. MacLeod, Hans Gunther Drexler;
ECV304 (endothelial) is really T24 (bladder carcinoma): cell line cross-contamination at source.
In Vitro Cell. Dev. Biol. Anim. 35:558-559(1999)

PubMed=10653001; DOI=10.1038/labinvest.3780006
Julia Brown, Sarah J. Reading, Sarah Jones, Caroline J. Fitchett, John Howl, Ashley Martin, Clare L. Longland, Francesco Michelangeli, Yuri E. Dubrova, Colin A. Brown;
Critical evaluation of ECV304 as a human endothelial cell model defined by genetic analysis and functional responses: a comparison with the human bladder cancer derived epithelial cell line T24/83.
Lab. Invest. 80:37-45(2000)

PubMed=11416159; DOI=10.1073/pnas.121616198; PMCID=PMC35459
John R.W. Masters, Jim A. Thomson, Bernadette Daly-Burns, Yvonne A. Reid, Wilhelm Gerhard Dirks, Phil Packer, Lorraine H. Toji, Tadao Ohno, Hideyuki Tanabe, Colin Francis Arlett, Lloyd R. Kelland ...Show all 16 authors... , Maureen Harrison, Arvind K. Virmani, Timothy H. Ward, Karen L. Ayres, Paul G. Debenham; Show fewer authors
Short tandem repeat profiling provides an international reference standard for human cell lines.
Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001)

PubMed=11669284; DOI=10.1290/1071-2690(2001)037<0505:PCOHUV>2.0.CO;2
Kayoshi Suda, Barbara Rothen-Rutishauser, Maja Gunthert, Heidi Wunderli-Allenspach;
Phenotypic characterization of human umbilical vein endothelial (ECV304) and urinary carcinoma (T24) cells: endothelial versus epithelial features.
In Vitro Cell. Dev. Biol. Anim. 37:505-514(2001)

PubMed=12197766; DOI=10.1290/1071-2690(2002)038<0185:BCCLEI>2.0.CO;2
Hans Gunther Drexler, Hilmar Quentmeier, Wilhelm Gerhard Dirks, Roderick A.F. MacLeod;
Bladder carcinoma cell line ECV304 is not a model system for endothelial cells.
In Vitro Cell. Dev. Biol. Anim. 38:185-186(2002)

PubMed=12453433; DOI=10.1006/mvre.2002.2434
Ronald E. Unger, Vera Krump-Konvalinkova, Kirsten Peters, Charles James Kirkpatrick;
In vitro expression of the endothelial phenotype: comparative study of primary isolated cells and cell lines, including the novel cell line HPMEC-ST1.6R.
Microvasc. Res. 64:384-397(2002)

PubMed=20143388; DOI=10.1002/ijc.25242
Amanda Capes-Davis, George Theodosopoulos, Isobel Atkin, Hans Gunther Drexler, Arihiro Kohara, Roderick A.F. MacLeod, John R.W. Masters, Yukio Nakamura, Yvonne A. Reid, Roger Robert Reddel, Robert Ian Freshney;
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

PubMed=27554326; DOI=10.1038/srep31811; PMCID=PMC4995472
Ya-Jun Xie, Jin-Long Wang, Yuan-Ya Zhang, Xiao-Fei Liu, Xiao-Rong Wang, Ke-Hui Liu, Xia-He Huang, Ying-Chun Wang;
Quantitative profiling of spreading-coupled protein tyrosine phosphorylation in migratory cells.
Sci. Rep. 6:31811.1-31811.10(2016)

PubMed=33389257; DOI=10.1007/s10096-020-04106-0; PMCID=PMC7778494
Nathalie Wurtz, Gwilherm Penant, Priscilla Jardot, Nathalie Duclos, Bernard La Scola;
Culture of SARS-CoV-2 in a panel of laboratory cell lines, permissivity, and differences in growth profile.
Eur. J. Clin. Microbiol. Infect. Dis. 40:477-484(2021)

Cross-references
Cell line collections (Providers) ATCC; CRL-1998 - Discontinued
BCRJ; 0283 - Discontinued
CLS; 300452
DSMZ; ACC-310 - Discontinued
ECACC; 92091712
IZSLER; BS CL 137
JCRB; JCRB0744
JCRB; NIHS0220 - Discontinued
Cell line databases/resources CLO; CLO_0002881
CLO; CLO_0002882
CLDB; cl1136
CLDB; cl5185
cancercelllines; CVCL_2029
DSMZCellDive; ACC-310
Anatomy/cell type resources BTO; BTO_0002043
Biological sample resources BioSample; SAMN03151937
BioSample; SAMN03471274
Chemistry resources ChEMBL-Cells; CHEMBL3307513
ChEMBL-Targets; CHEMBL614035
PubChem_Cell_line; CVCL_2029
Encyclopedic resources Wikidata; Q54831948
Experimental variables resources EFO; EFO_0022389
Polymorphism and mutation databases Cosmic; 1466801
Proteomic databases PRIDE; PXD004340
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number38