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Cellosaurus MDA-MB-435S (CVCL_0622)

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Cell line name MDA-MB-435S
Synonyms MDA-MB-435s; MDA-MB-435 S; MDA-MB-435-S; MDAMB435S; BrCL15
Accession CVCL_0622
Resource Identification Initiative To cite this cell line use: MDA-MB-435S (RRID:CVCL_0622)
Comments Problematic cell line: Contaminated. Parent cell line (MDA-MB-435) has been shown to be a M14 derivative.
Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: KuDOS 95 cell line panel.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00565.
Population: Caucasian.
Microsatellite instability: Stable (MSS) (PubMed=12661003).
Omics: Array-based CGH.
Omics: Deep exome analysis.
Omics: miRNA expression profiling.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Derived from site: Metastatic; Right buttock, hypodermis; UBERON=UBERON_0013691+UBERON_0002072.
Sequence variations
  • Mutation; HGNC; 1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (PubMed=19593635; DepMap=ACH-000884).
  • Mutation; HGNC; 1787; CDKN2A; Simple; c.150+2T>C (IVS1+2T>C); ClinVar=VCV000406712; Zygosity=Heterozygous; Note=Splice donor mutation (PubMed=19593635).
  • Mutation; HGNC; 1787; CDKN2A; Simple; c.455insCdel26; Zygosity=Heterozygous (PubMed=19593635).
  • Mutation; HGNC; 3373; EP300; Simple; p.Leu827Pro (c.2480T>C); Zygosity=Hemizygous (PubMed=10700188).
  • Mutation; HGNC; 3373; EP300; Simple; p.Glu1013Gly (c.3038A>G); ClinVar=VCV000562024; Zygosity=Hemizygous (PubMed=10700188).
  • Mutation; HGNC; 11998; TP53; Simple; p.Gly266Glu (c.797G>A); ClinVar=VCV000161516; Zygosity=Heterozygous (PubMed=16541312; DepMap=ACH-000884).
HLA typing Source: PubMed=26589293
Class I
Genome ancestry Source: PubMed=30894373

Origin% genome
Native American0
East Asian, North2.13
East Asian, South0
South Asian0
European, North65.23
European, South32.63
Disease Amelanotic melanoma (NCIt: C3802)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0417 (MDA-MB-435)
CVCL_A6LJ (MDA-MB-435S/1)CVCL_5T70 (MDA-MB-435s-mKate2)
Sex of cell Male
Age at sampling 33Y
Category Cancer cell line
STR profile Source(s): ATCC=HTB-129; CCRID; CLS=300277; PubMed=28940260

CSF1PO11 (ATCC=HTB-129; CCRID; CLS=300277)
11,12 (PubMed=28940260)
D3S135814 (ATCC=HTB-129; CLS=300277; PubMed=28940260)
14,20 (CCRID)
D5S81811,12 (CCRID)
12 (ATCC=HTB-129; CLS=300277; PubMed=28940260)
D7S8208 (PubMed=28940260)
8,10 (ATCC=HTB-129; CCRID; CLS=300277)
D8S117913 (ATCC=HTB-129; CCRID; CLS=300277)
13,14 (PubMed=28940260)
D13S31712 (ATCC=HTB-129; CCRID; PubMed=28940260)
12,13 (CLS=300277)
D18S5113 (ATCC=HTB-129; PubMed=28940260)
13,17 (CLS=300277)
D19S43314 (CCRID)
14,15 (ATCC=HTB-129; CLS=300277; PubMed=28940260)
Penta D9,11
Penta E10,12

Run an STR similarity search on this cell line
Web pages https://www.atcc.org/en/support/technical-support/faqs/atcc-htb-129-derivative

PubMed=2007622; DOI=10.1083/jcb.113.1.173
Frixen U.H., Behrens J., Sachs M., Eberle G., Voss B., Warda A., Lochner D., Birchmeier W.
E-cadherin-mediated cell-cell adhesion prevents invasiveness of human carcinoma cells.
J. Cell Biol. 113:173-185(1991)

Leibovitz A.
Cell lines from human breast.
(In) Atlas of human tumor cell lines; Hay R.J., Park J.-G., Gazdar A.F. (eds.); pp.161-184; Academic Press; New York (1994)

PubMed=10700188; DOI=10.1038/73536
Gayther S.A., Batley S.J., Linger L., Bannister A.J., Thorpe K., Chin S.-F., Daigo Y., Russell P., Wilson A., Sowter H.M., Delhanty J.D.A., Ponder B.A.J., Kouzarides T., Caldas C.
Mutations truncating the EP300 acetylase in human cancers.
Nat. Genet. 24:300-303(2000)

PubMed=12354931; DOI=10.1136/mp.55.5.294
Ellison G., Klinowska T., Westwood R.F.R., Docter E., French T., Fox J.C.
Further evidence to support the melanocytic origin of MDA-MB-435.
Mol. Pathol. 55:294-299(2002)

PubMed=12661003; DOI=10.1002/gcc.10196
Seitz S., Wassmuth P., Plaschke J., Schackert H.K., Karsten U., Santibanez-Koref M.F., Schlag P.M., Scherneck S.
Identification of microsatellite instability and mismatch repair gene mutations in breast cancer cell lines.
Genes Chromosomes Cancer 37:29-35(2003)

PubMed=15677628; DOI=10.1093/carcin/bgi032
Gorringe K.L., Chin S.-F., Pharoah P.D.P., Staines J.M., Oliveira C., Edwards P.A.W., Caldas C.
Evidence that both genetic instability and selection contribute to the accumulation of chromosome alterations in cancer.
Carcinogenesis 26:923-930(2005)

PubMed=16397213; DOI=10.1158/0008-5472.CAN-05-2853
Elstrodt F., Hollestelle A., Nagel J.H.A., Gorin M., Wasielewski M., van den Ouweland A.M.W., Merajver S.D., Ethier S.P., Schutte M.
BRCA1 mutation analysis of 41 human breast cancer cell lines reveals three new deleterious mutants.
Cancer Res. 66:41-45(2006)

PubMed=16541312; DOI=10.1007/s10549-006-9186-z
Wasielewski M., Elstrodt F., Klijn J.G.M., Berns E.M.J.J., Schutte M.
Thirteen new p53 gene mutants identified among 41 human breast cancer cell lines.
Breast Cancer Res. Treat. 99:97-101(2006)

PubMed=19593635; DOI=10.1007/s10549-009-0460-8
Hollestelle A., Nagel J.H.A., Smid M., Lam S., Elstrodt F., Wasielewski M., Ng S.S., French P.J., Peeters J.K., Rozendaal M.J., Riaz M., Koopman D.G., ten Hagen T.L.M., de Leeuw B.H.C.G.M., Zwarthoff E.C., Teunisse A., van der Spek P.J., Klijn J.G.M., Dinjens W.N.M., Ethier S.P., Clevers H.C., Jochemsen A.G., den Bakker M.A., Foekens J.A., Martens J.W.M., Schutte M.
Distinct gene mutation profiles among luminal-type and basal-type breast cancer cell lines.
Breast Cancer Res. Treat. 121:53-64(2010)

PubMed=20070913; DOI=10.1186/1471-2407-10-15
Tsuji K., Kawauchi S., Saito S., Furuya T., Ikemoto K., Nakao M., Yamamoto S., Oka M., Hirano T., Sasaki K.
Breast cancer cell lines carry cell line-specific genomic alterations that are distinct from aberrations in breast cancer tissues: comparison of the CGH profiles between cancer cell lines and primary cancer tissues.
BMC Cancer 10:15.1-15.10(2010)

PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.-Y.K., Yu J.-J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N.-X., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M.L., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=23601657; DOI=10.1186/bcr3415
Riaz M., van Jaarsveld M.T.M., Hollestelle A., Prager-van der Smissen W.J.C., Heine A.A.J., Boersma A.W.M., Liu J.-J., Helmijr J.C.A., Ozturk B., Smid M., Wiemer E.A.C., Foekens J.A., Martens J.W.M.
miRNA expression profiling of 51 human breast cancer cell lines reveals subtype and driver mutation-specific miRNAs.
Breast Cancer Res. 15:R33.1-R33.17(2013)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5
Scholtalbers J., Boegel S., Bukur T., Byl M., Goerges S., Sorn P., Loewer M., Sahin U., Castle J.C.
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=28940260; DOI=10.1002/ijc.31067
Korch C.T., Hall E.M., Dirks W.G., Ewing M., Faries M., Varella-Garcia M., Robinson S., Storts D.R., Turner J.A., Wang Y., Burnett E.C., Healy L.E., Kniss D.A., Neve R.M., Nims R.W., Reid Y.A., Robinson W.A., Capes-Davis A.
Authentication of M14 melanoma cell line proves misidentification of MDA-MB-435 breast cancer cell line.
Int. J. Cancer 142:561-572(2017)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

Cell line collections (Providers) ATCC; HTB-129
BCRJ; 0165
CLS; 300277 - Discontinued
ICLC; HTL03006
KCB; KCB 200773YJ
Cell line databases/resources CLO; CLO_0007638
CLDB; cl7142
cancercelllines; CVCL_0622
CCRID; 1101HUM-PUMC000015
Cell_Model_Passport; SIDM01222
DepMap; ACH-000884
LINCS_HMS; 50030
Anatomy/cell type resources BTO; BTO:0003941
Biological sample resources BioSample; SAMN10987989
CRISP screens repositories BioGRID_ORCS_Cell_line; 567
Chemistry resources ChEMBL-Cells; CHEMBL4483174
ChEMBL-Targets; CHEMBL4483284
PubChem_Cell_line; CVCL_0622
Encyclopedic resources Wikidata; Q54904637
Gene expression databases ArrayExpress; E-MTAB-2770
GEO; GSM149983
GEO; GSM149991
GEO; GSM149999
GEO; GSM421877
GEO; GSM887298
GEO; GSM888373
GEO; GSM1374657
Polymorphism and mutation databases Cosmic; 809237
Cosmic; 871155
Cosmic; 894089
Cosmic; 904381
Cosmic; 1044202
Cosmic; 1046951
Cosmic; 1287922
Cosmic; 1289399
Cosmic; 1523969
Cosmic; 1609477
Cosmic; 2301531
LiGeA; CCLE_408
Progenetix; CVCL_0622
Proteomic databases PRIDE; PXD016837
Entry history
Entry creation04-Apr-2012
Last entry update02-May-2024
Version number41