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Cellosaurus MDA-MB-435s-mKate2 (CVCL_5T70)

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Cell line name MDA-MB-435s-mKate2
Accession CVCL_5T70
Resource Identification Initiative To cite this cell line use: MDA-MB-435s-mKate2 (RRID:CVCL_5T70)
Comments Problematic cell line: Contaminated. Grand-parent cell line (MDA-MB-435) has been shown to be a M14 derivative.
Population: Caucasian.
Genetic integration: Method=Transfection; Gene=FPbase; DBBO8; mKate2 (Note=Bright red fluorescent protein derived from eqFP578).
Derived from site: Metastatic; Right buttock, hypodermis; UBERON=UBERON_0013691+UBERON_0002072.
Sequence variations
  • Mutation; HGNC; HGNC:1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:1787; CDKN2A; Simple; c.150+2T>C (IVS1+2T>C); ClinVar=VCV000406712; Zygosity=Heterozygous; Note=Splice donor mutation (from parent cell line).
  • Mutation; HGNC; HGNC:1787; CDKN2A; Simple; c.455insCdel26; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Gly266Glu (c.797G>A); ClinVar=VCV000161516; Zygosity=Heterozygous (from parent cell line).
Disease Amelanotic melanoma (NCIt: C3802)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0622 (MDA-MB-435S)
Sex of cell Male
Age at sampling 33Y
Category Cancer cell line
Publications

PubMed=25902968; DOI=10.1007/s11307-015-0853-5
Ivan Vuletic, Jing-Hao Liu, Hong-Lian Wu, Yi-Chen Ding, Yu Lei, Chang-Hui Li, De-Sheng Zhu, Qiu-Shi Ren, Hong-Fang Sun, Jun Li;
Establishment of an mKate2-expressing cell line for non-invasive real-time breast cancer in vivo imaging.
Mol. Imaging Biol. 17:811-818(2015)

Cross-references
Cell line databases/resources cancercelllines; CVCL_5T70
Encyclopedic resources Wikidata; Q54904638
Entry history
Entry creation23-Feb-2016
Last entry update19-Dec-2024
Version number20