Cellosaurus Sf9 (CVCL_0549)
Cell line name | Sf9 | |||||||||||||||||||||
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Synonyms | SF9; sf9; SF-9; Sf-9; sf-9; Sf 9; Spodoptera frugiperda clone 9; Sf clone 9; IPLB-Sf-9AE; IPLB-SF-9AE; IPLB-SF-9; IPLB-Sf-9; IPLB-Sf9 | |||||||||||||||||||||
Accession | CVCL_0549 | |||||||||||||||||||||
Resource Identification Initiative | To cite this cell line use: Sf9 (RRID:CVCL_0549) | |||||||||||||||||||||
Comments | Group: Insect cell line. Group: Recombinant protein production insect cell line. Group: Space-flown cell line (cellonaut). Group: Vaccine production cell line. Biotechnology: Used for the production of the Novavax SARS-CoV-2 (COVID-19) vaccine (Trade name: NVX-CoV2373) (DrugBank; DB15810). The cell line is used to produce the recombinant SARS-CoV-2 spike protein rSARS-CoV-2 which is the active part of the vaccine formulation. Doubling time: 25.2 +- 2.8 hours (Note=At 28 Celsius) (PubMed=10718368); 27 hours (PubMed=21667340); ~50 hours (DSMZ=ACC-125). Karyotypic information: Heterogeneous, contains a mixture of diploid and tetraploid cells. Omics: Transcriptome analysis by ESTs sequencing. Omics: Transcriptome analysis by microarray. Omics: Genome sequenced. Anecdotal: Have been flown in space on shuttle flights STS-50 (USML-1), STS-54 and STS-57 (Spacehab-1) to study growth in microgravity (PubMed=8050503). Caution: The SfRV rhabdovirus found in Sf9 cells of ATCC CRL-1711 lot 5807852 (PubMed=24672045) does not seem to be expressed in CRL-1711 lot 5814 from 1987 (PubMed=28423032). The SfRV genome is integrated in the Sf9 genome. Derived from site: In situ; Ovary; UBERON=UBERON_0000992. | |||||||||||||||||||||
Species of origin | Spodoptera frugiperda (Fall armyworm) (NCBI Taxonomy: 7108) | |||||||||||||||||||||
Hierarchy | Parent: CVCL_0518 (Sf21) Children:
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Sex of cell | Female | |||||||||||||||||||||
Age at sampling | Pupal stage | |||||||||||||||||||||
Category | Spontaneously immortalized cell line | |||||||||||||||||||||
Web pages | https://en.wikipedia.org/wiki/Sf9_(cells) https://www.atcc.org/en/support/technical-support/faqs/morphology-of-atcc-crl-1711 https://www.thermofisher.com/order/catalog/product/B82501 https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/s/cell-lines-detail-311.html https://www.ars.usda.gov/sp2userfiles/person/3489/sf9.pdf https://oetltd.com/product/sf9-cells/ http://www.the-scientist.com/?articles.view/articleNo/40052/title/Virus-Found-in-Sf9-Cell-Line/ | |||||||||||||||||||||
Publications | PubMed=1367489; DOI=10.1021/bp00007a002 PubMed=1369220; DOI=10.1021/bp00017a003 PubMed=8314732; DOI=10.1007/BF02633986 PubMed=8050503; DOI=10.1006/excr.1994.1223 PubMed=7615077; DOI=10.1016/0014-5793(95)00640-u PubMed=8799737; DOI=10.1111/j.1365-2583.1996.tb00053.x PubMed=10718368; DOI=10.1290/1071-2690(2000)036<0117:aoaicl>2.0.co;2 PubMed=10777062; DOI=10.1290/1071-2690(2000)036<0205:EOTGFP>2.0.CO;2 DOI=10.11416/jibs2001.71.25 PubMed=12052082; DOI=10.1021/bp020028 PubMed=14668217; DOI=10.1093/bioinformatics/btg324 PubMed=19003226; DOI=10.1023/B:CYTO.0000043394.40309.48 PubMed=15926859; DOI=10.1290/0412081.1 DOI=10.3923/jas.2007.4040.4043 DOI=10.1111/j.1439-0418.2010.01574.x PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022 PubMed=20300881; DOI=10.1007/s12033-010-9268-3 PubMed=21667340; DOI=10.1007/s12250-011-3177-x PubMed=23891577; DOI=10.1016/j.tiv.2013.07.007 PubMed=24375231; DOI=10.1007/s10529-013-1429-6 PubMed=24672045; DOI=10.1128/JVI.00780-14 PubMed=28423032; DOI=10.1371/journal.pone.0175633 PubMed=28839023; DOI=10.1128/genomeA.00829-17 PubMed=29038528; DOI=10.1038/s41598-017-12713-9 PubMed=29133148; DOI=10.1016/j.pep.2017.11.002 | |||||||||||||||||||||
Cross-references | ||||||||||||||||||||||
Cell line collections (Providers) | ATCC; CRL-1711
BCRC; 60011 CCTCC; GDC0008 CCLV; CCLV-RIE 0352 CLS; 604328 DSMZ; ACC-125 ECACC; 89070101 IBRC; C10127 IZSLER; BS CL 157 KCB; KCB 92018YJ KCB; KCB 200807YJ NCBI_Iran; C425 NISES; SK0002 RCB; RCB0563 - Discontinued | |||||||||||||||||||||
Cell line databases/resources | CLO; CLO_0008988
CLO; CLO_0008989 CLO; CLO_0008990 MCCL; MCC:0000450 CLDB; cl4277 CLDB; cl4278 CLDB; cl4279 CCRID; 1101INS-PUMC000117 CCRID; 1102INS-NIFDC00107 CCRID; 4201INS-CCTCC00008 DSMZCellDive; ACC-125 ICLDB; sf9 Lonza; 1014 TOKU-E; 3107 | |||||||||||||||||||||
Anatomy/cell type resources | BTO; BTO:0001240 | |||||||||||||||||||||
Chemistry resources | ChEMBL-Cells; CHEMBL3308860
PubChem_Cell_line; CVCL_0549 | |||||||||||||||||||||
Encyclopedic resources | Wikidata; Q7459700 | |||||||||||||||||||||
Medical resources | MeSH; D061987 | |||||||||||||||||||||
Entry history | ||||||||||||||||||||||
Entry creation | 04-Apr-2012 | |||||||||||||||||||||
Last entry update | 29-Jun-2023 | |||||||||||||||||||||
Version number | 36 |