Cellosaurus logo
expasy logo

Cellosaurus Sf21 (CVCL_0518)

[Text version]
Cell line name Sf21
Synonyms SF21; Sf-21; SF-21; IPLB-SF-21-AE; IPLB-Sf21-AE; IPLB-SF21-AE; IPLB-SF 21AE; IPLB-Sf21AE; IPLB-SF21AE; IPLB-SF-21; IPLB-Sf-21; IPLB-SF 21; IPLB-Sf21; IPLB-SF21
Accession CVCL_0518
Resource Identification Initiative To cite this cell line use: Sf21 (RRID:CVCL_0518)
Comments Group: Insect cell line.
Group: Recombinant protein production insect cell line.
Doubling time: 26-30 hours (PubMed=68913); 33 +- 1 hours (Note=In static culture), 26 +- 1 hours (Note=In suspension culture) (PubMed=10585188); ~3 days (DSMZ=ACC-119); 24 hours (ECACC=05022801).
Misspelling: IPBL-SF-21AE; Note=Occasionally.
Misspelling: IPBL-SF-21; Note=Occasionally.
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Species of origin Spodoptera frugiperda (Fall armyworm) (NCBI Taxonomy: 7108)
Hierarchy Children:
CVCL_C2TG (IBL-Sf-21AE-Cl 1)CVCL_C2TJ (IBL-Sf-21AE-Cl 10)CVCL_C2TK (IBL-Sf-21AE-Cl 13)
CVCL_C2TM (IBL-Sf-21AE-Cl 14)CVCL_C2TN (IBL-Sf-21AE-Cl 15)CVCL_C2TL (IBL-Sf-21AE-Cl 18)
CVCL_C2TH (IBL-Sf-21AE-Cl 2)CVCL_Z451 (IBL-Sf-21AE-Cl 3)CVCL_Z452 (IBL-Sf-21AE-Cl 5)
CVCL_C2TI (IBL-Sf-21AE-Cl 7)CVCL_Z453 (IBL-Sf-21AE-Cl 9)CVCL_A7NB (Sf-21-CB)
CVCL_2074 (Sf158)CVCL_JX37 (Sf21 TiterHigh AC free)CVCL_A2IN (Sf21-HT)
CVCL_0549 (Sf9)
Sex of cell Female
Age at sampling Pupal stage
Category Spontaneously immortalized cell line
Web pages Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/s/cell-lines-detail-382.html
Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/s/cell-lines-detail-385.html
Info; USDA-ARS; -; https://www.ars.usda.gov/sp2userfiles/person/3489/sf21.pdf
Info; Wikipedia; -; https://en.wikipedia.org/wiki/Sf21
Provider; OET; -; https://oetltd.com/product/sf21-cells/
Publications

DOI=10.1016/B978-0-12-709902-6.50017-0
Walter Frederic Hink;
A catalog of invertebrate cell lines.
(In book chapter) Invertebrate tissue culture, Vol. II; Vago, Constantin (eds.); pp.363-387; Academic Press; New York; USA (1972)

PubMed=68913; DOI=10.1007/BF02615077
James Lloyd Vaughn, Ronald H. Goodwin, George J. Tompkins, Patricia E. McCawley;
The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae).
In Vitro 13:213-217(1977)

PubMed=16346059; DOI=10.1128/AEM.44.1.227-233.1982; PMCID=PMC241993
Loy E. Volkman, Phyllis A. Goldsmith;
Generalized immunoassay for Autographa californica nuclear polyhedrosis virus infectivity in vitro.
Appl. Environ. Microbiol. 44:227-233(1982)

CLPUB00492
Jun Mitsuhashi;
Nutritional requirements of insect cells in vitro.
(In book chapter) Invertebrate cell system applications, Vol. I; Mitsuhashi, Jun (eds.); pp.3-20; CRC Press; Boca Raton; USA (1989)

PubMed=1367489; DOI=10.1021/bp00007a002
Walter Frederic Hink, Darrell R. Thomsen, Don J. Davidson, Annette L. Meyer, Francis J. Castellino;
Expression of three recombinant proteins using baculovirus vectors in 23 insect cell lines.
Biotechnol. Prog. 7:9-14(1991)

PubMed=1369220; DOI=10.1021/bp00017a003
Thomas J. Wickham, Thomas R. Davis, Robert R. Granados, Michael Louis Shuler, H. Alan Wood;
Screening of insect cell lines for the production of recombinant proteins and infectious virus in the baculovirus expression system.
Biotechnol. Prog. 8:391-396(1992)

PubMed=8314732; DOI=10.1007/BF02633986
Thomas R. Davis, Thomas J. Wickham, Kevin A. McKenna, Robert R. Granados, Michael Louis Shuler, H. Alan Wood;
Comparative recombinant protein production of eight insect cell lines.
In Vitro Cell. Dev. Biol. Anim. 29:388-390(1993)

DOI=10.19603/j.cnki.1000-1190.1995.04.025
Lu-Lin Li, Qu-Hou Chen;
Storage of insect cell lines by a simple method.
Hua Zhong Shi Fan Da Xue Xue Bao. Zi Ran Ke Xue Ban 29:511-512(1995)

PubMed=8799737; DOI=10.1111/j.1365-2583.1996.tb00053.x
Arthur H. McIntosh, James J. Grasela, Robert L. Matteri;
Identification of insect cell lines by DNA amplification fingerprinting (DAF).
Insect Mol. Biol. 5:187-195(1996)

PubMed=10475266; DOI=10.1007/s11626-999-0010-z
Dawn E. Gundersen-Rindal, Dwight E. Lynn, Edward M. Dougherty;
Transformation of lepidopteran and coleopteran insect cell lines by Glyptapanteles indiensis polydnavirus DNA.
In Vitro Cell. Dev. Biol. Anim. 35:111-114(1999)

PubMed=10585188; DOI=10.1021/bp990119f
Michelle B. Keith, Patrick J. Farrell, Kostas Iatrou, Leo Augustus Behie;
Screening of transformed insect cell lines for recombinant protein production.
Biotechnol. Prog. 15:1046-1052(1999)

PubMed=10777062; DOI=10.1290/1071-2690(2000)036<0205:EOTGFP>2.0.CO;2
James J. Grasela, Arthur H. McIntosh, Cynthia Lenz Goodman, Louise E. Wilson, Leslie A. King;
Expression of the green fluorescent protein carried by Autographa californica multiple nucleopolyhedrovirus in insect cell lines.
In Vitro Cell. Dev. Biol. Anim. 36:205-210(2000)

PubMed=10852353; DOI=10.1290/1071-2690(2000)036<0271:IVHRSW>2.0.CO;2
Charles W. Kariuki, Arthur H. McIntosh, Cynthia Lenz Goodman;
In vitro host range studies with a new baculovirus isolate from the diamondback moth Plutella xylostella (L.) (Plutellidae: Lepidoptera).
In Vitro Cell. Dev. Biol. Anim. 36:271-276(2000)

PubMed=12623313; DOI=10.1016/S0022-2011(03)00002-8
Dwight E. Lynn;
Comparative susceptibilities of twelve insect cell lines to infection by three baculoviruses.
J. Invertebr. Pathol. 82:129-131(2003)

PubMed=15038778; DOI=10.1290/1543-706X(2003)039<0353:AOISRT>2.0.CO;2
James J. Grasela, Arthur H. McIntosh;
Application of inter-simple sequence repeats to insect cell lines: identification at the clonal and tissue-specific level.
In Vitro Cell. Dev. Biol. Anim. 39:353-363(2003)

PubMed=15926859; DOI=10.1290/0412081.1
Yan-Ping Chen, Dawn E. Gundersen-Rindal, Dwight E. Lynn;
Baculovirus-based expression of an insect viral protein in 12 different insect cell lines.
In Vitro Cell. Dev. Biol. Anim. 41:43-49(2005)

PubMed=16409117; DOI=10.1290/0412083R.1
Arthur H. McIntosh, James J. Grasela, Holly J.R. Popham;
AcMNPV in permissive, semipermissive, and nonpermissive cell lines from Arthropoda.
In Vitro Cell. Dev. Biol. Anim. 41:298-304(2005)

PubMed=22797937; DOI=10.1007/s10158-012-0138-5
Lacey J. Jenson, Sally L. Paulson, Jeffrey R. Bloomquist;
Induction and inhibition of an apparent neuronal phenotype in Spodoptera frugiperda insect cells (Sf21) by chemical agents.
Invert. Neurosci. 12:119-127(2012)

PubMed=24672045; DOI=10.1128/JVI.00780-14; PMCID=PMC4054387
Hai-Lun Ma, Teresa A. Galvin, Dustin R. Glasner, Syed Shaheduzzaman, Arifa S. Khan;
Identification of a novel rhabdovirus in Spodoptera frugiperda cell lines.
J. Virol. 88:6576-6585(2014)

PubMed=29133148; DOI=10.1016/j.pep.2017.11.002; PMCID=PMC5826799
Christoph Geisler, Donald L. Jarvis;
Adventitious viruses in insect cell lines used for recombinant protein expression.
Protein Expr. Purif. 144:25-32(2018)

Cross-references
Cell line collections (Providers) CCTCC; GDC0020
DSMZ; ACC-119
ECACC; 05022801
KCB; KCB 2010008YJ
NCBI_Iran; C503
Cell line databases/resources CLO; CLO_0008987
MCCL; MCC:0000416
CLDB; cl2906
CCRID; 4201INS-CCTCC00020
DSMZCellDive; ACC-119
ICLDB; iplb-sf21
Lonza; 1183
Anatomy/cell type resources BTO; BTO_0001196
Chemistry resources ChEMBL-Cells; CHEMBL4106362
PubChem_Cell_line; CVCL_0518
Encyclopedic resources Wikidata; Q2276068
Experimental variables resources EFO; EFO_0022744
Entry history
Entry creation04-Apr-2012
Last entry update14-Aug-2025
Version number33