Cellosaurus logo
expasy logo

Cellosaurus NCI-H295R (CVCL_0458)

[Text version]
Cell line name NCI-H295R
Synonyms NCI H295R; NCIH295R; H295R; H-295R; H295R-S1
Accession CVCL_0458
Resource Identification Initiative To cite this cell line use: NCI-H295R (RRID:CVCL_0458)
Comments Characteristics: Grows in a medium called Nu-Serum type I.
Doubling time: 25 hours (PubMed=29371329).
Omics: Genomics; Whole genome sequencing.
Omics: Transcriptomics; RNAseq.
Omics: Variations; SNP array analysis.
Derived from site: In situ; Adrenal gland; UBERON=UBERON_0002369.
Sequence variations
  • Mutation; HGNC; HGNC:2514; CTNNB1; Simple; p.Ser45Pro (c.133T>C); ClinVar=VCV000017589; Zygosity=Heterozygous (from parent cell line).
Disease Adrenal cortex carcinoma (NCIt: C9325)
Adrenocortical carcinoma (ORDO: Orphanet_1501)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0456 (NCI-H295)
Children:
CVCL_5V09 (HAC13)CVCL_S898 (HAC15)CVCL_5V08 (HAC50)
Sex of cell Female
Age at sampling 48Y
Category Cancer cell line
STR profile Source(s): ATCC=CRL-2128; CCRID; CLS=300483

Markers:
AmelogeninX
CSF1PO10,12
D2S133825
D3S135815,16
D5S81812
D7S8209,12
D8S117913
D13S31713
D16S53911
D18S5117
D19S43313
D21S1132.2
FGA19.2,24
Penta D8
Penta E5,12
TH019.3
TPOX8
vWA17,18

Run an STR similarity search on this cell line
Web pages Info; Wikipedia; -; https://en.wikipedia.org/wiki/H295R
Publications

PubMed=15541570; DOI=10.1016/j.mce.2003.12.020
William E. Rainey, Karla Saner, Bernard P. Schimmer;
Adrenocortical cell lines.
Mol. Cell. Endocrinol. 228:23-38(2004)

PubMed=18000308; DOI=10.1677/JOE-07-0166
Elika Samandari, Petra Kempna, Jean-Marc Nuoffer, Gaby Hofer, Primus-Eugen Mullis, Christa E. Fluck;
Human adrenal corticocarcinoma NCI-H295R cells produce more androgens than NCI-H295A cells and differ in 3beta-hydroxysteroid dehydrogenase type 2 and 17,20 lyase activities.
J. Endocrinol. 195:459-472(2007)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662
S. Michael Rothenberg, Gayatry Mohapatra, Miguel N. Rivera, Daniel Winokur, Patricia Greninger, Mai Nitta, Peter M. Sadow, Gaya Sooriyakumar, Brian W. Brannigan, Matthew J. Ulman, Rushika M. Perera ...Show all 22 authors... , Rui Wang, Angela Tam, Xiao-Jun Ma, Mark Erlander, Dennis C. Sgroi, James W. Rocco, Mark W. Lingen, Ezra E.W. Cohen, David Neil Louis, Jeffrey Settleman, Daniel Arie Haber; Show fewer authors
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=21924324; DOI=10.1016/j.mce.2011.08.041; PMCID=PMC3288152
Tao Wang, William E. Rainey;
Human adrenocortical carcinoma cell lines.
Mol. Cell. Endocrinol. 351:58-65(2012)

PubMed=22266826; DOI=10.1055/s-0031-1298019; PMCID=PMC4269336
Tao Wang, Jennifer G. Rowland, Jeniel Parmar, Maria Nesterova, Tsugio Seki, William E. Rainey;
Comparison of aldosterone production among human adrenocortical cell lines.
Horm. Metab. Res. 44:245-250(2012)

PubMed=23951072; DOI=10.1371/journal.pone.0071022; PMCID=PMC3738630
Haruhisa Nishi, Hirokazu Arai, Toshihiko Momiyama;
NCI-H295R, a human adrenal cortex-derived cell line, expresses purinergic receptors linked to Ca(2)(+)-mobilization/influx and cortisol secretion.
PLoS ONE 8:e71022.1-e71022.13(2013)

PubMed=29371329; DOI=10.1530/ERC-17-0447; PMCID=PMC5831504
Katja Kiseljak-Vassiliades, Yu Zhang, Stacey M. Bagby, Adwitiya Kar, Nikita Pozdeyev, Mei Xu, Katherine Gowan, Vibha Sharma, Christopher D. Raeburn, Maria Belen Albuja-Cruz, Kenneth L. Jones ...Show all 17 authors... , Lauren Fishbein, Rebecca E. Schweppe, Hilary Somerset, Todd M. Pitts, Stephen Leong, Margaret E. Wierman; Show fewer authors
Development of new preclinical models to advance adrenocortical carcinoma research.
Endocr. Relat. Cancer 25:437-451(2018)

PubMed=33981288; DOI=10.3389/fendo.2021.669426; PMCID=PMC8108132
Elisa Rossini, Mariangela Tamburello, Andrea Abate, Silvia Beretta, Martina Fragni, Manuela Cominelli, Deborah Cosentini, Constanze Hantel, Federica Bono, Salvatore Grisanti, Pietro Luigi Poliani ...Show all 15 authors... , Guido Alberto Massimo Tiberio, Maurizio Memo, Sandra Sigala, Alfredo Berruti; Show fewer authors
Cytotoxic effect of progesterone, tamoxifen and their combination in experimental cell models of human adrenocortical cancer.
Front. Endocrinol. 12:669426.1-669426.13(2021)

PubMed=34572026; DOI=10.3390/cells10092376; PMCID=PMC8466536
Kimberly Wellman, Rui Fu, Amber Baldwin, Juilee Rege, Elisabeth Murphy, William E. Rainey, Neelanjan Mukherjee;
Transcriptomic response dynamics of human primary and immortalized adrenocortical cells to steroidogenic stimuli.
Cells 10:2376.1-2376.15(2021)

PubMed=35563746; DOI=10.3390/cells11091439; PMCID=PMC9103477
Sandra Sigala, Christina Bothou, David Penton, Andrea Abate, Mirko Peitzsch, Deborah Cosentini, Guido Alberto Massimo Tiberio, Stefan Richard Bornstein, Alfredo Berruti, Constanze Hantel;
A comprehensive investigation of steroidogenic signaling in classical and new experimental cell models of adrenocortical carcinoma.
Cells 11:1439.1-1439.17(2022)

PubMed=35764904; DOI=10.1007/s12020-022-03112-w; PMCID=PMC9385758
Sandra Sigala, Elisa Rossini, Andrea Abate, Mariangela Tamburello, Stefan Richard Bornstein, Constanze Hantel;
An update on adrenocortical cell lines of human origin.
Endocrine 77:432-437(2022)

Cross-references
Cell line collections (Providers) ATCC; CRL-2128
CLS; 300483
KCB; KCB 2015009YJ
Cell line databases/resources CLO; CLO_0008082
MCCL; MCC:0000355
cancercelllines; CVCL_0458
CCRID; 1101HUM-PUMC000256
CCRID; 5301HUM-KCB15009YJ
IGRhCellID; NCIH295R
Lonza; 378
Anatomy/cell type resources BTO; BTO_0002588
Chemistry resources ChEMBL-Cells; CHEMBL3307998
ChEMBL-Targets; CHEMBL612593
PubChem_Cell_line; CVCL_0458
Encyclopedic resources Wikidata; Q25323725
Experimental variables resources EFO; EFO_0002289
Gene expression databases ArrayExpress; E-MTAB-38
Polymorphism and mutation databases Cosmic; 1172633
Cosmic; 1191070
Cosmic; 1622873
Cosmic; 2364101
Cosmic; 2574494
Progenetix; CVCL_0458
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number30