ID   NCI-H295R
AC   CVCL_0458
SY   NCI H295R; NCIH295R; H295R; H-295R; H295R-S1
DR   BTO; BTO_0002588
DR   CLO; CLO_0008082
DR   EFO; EFO_0002289
DR   MCCL; MCC:0000355
DR   ArrayExpress; E-MTAB-38
DR   ATCC; CRL-2128
DR   cancercelllines; CVCL_0458
DR   CCRID; 1101HUM-PUMC000256
DR   CCRID; 5301HUM-KCB15009YJ
DR   ChEMBL-Cells; CHEMBL3307998
DR   ChEMBL-Targets; CHEMBL612593
DR   CLS; 300483
DR   Cosmic; 1172633
DR   Cosmic; 1191070
DR   Cosmic; 1622873
DR   Cosmic; 2364101
DR   Cosmic; 2574494
DR   IGRhCellID; NCIH295R
DR   KCB; KCB 2015009YJ
DR   Lonza; 378
DR   Progenetix; CVCL_0458
DR   PubChem_Cell_line; CVCL_0458
DR   Wikidata; Q25323725
RX   PubMed=15541570;
RX   PubMed=18000308;
RX   PubMed=20215515;
RX   PubMed=21924324;
RX   PubMed=22266826;
RX   PubMed=23951072;
RX   PubMed=29371329;
RX   PubMed=33981288;
RX   PubMed=34572026;
RX   PubMed=35563746;
RX   PubMed=35764904;
WW   Info; Wikipedia; -; https://en.wikipedia.org/wiki/H295R
CC   Population: Caribbean; Bahamas.
CC   Characteristics: Grows in a medium called Nu-Serum type I.
CC   Doubling time: 25 hours (PubMed=29371329).
CC   Sequence variation: Mutation; HGNC; HGNC:2514; CTNNB1; Simple; p.Ser45Pro (c.133T>C); ClinVar=VCV000017589; Zygosity=Heterozygous (from parent cell line).
CC   Omics: Genomics; Whole genome sequencing.
CC   Omics: Transcriptomics; RNAseq.
CC   Omics: Variations; SNP array analysis.
CC   Derived from site: In situ; Adrenal gland; UBERON=UBERON_0002369.
ST   Source(s): ATCC=CRL-2128; CCRID; CLS=300483
ST   Amelogenin: X
ST   CSF1PO: 10,12
ST   D13S317: 13
ST   D16S539: 11
ST   D18S51: 17
ST   D19S433: 13
ST   D21S11: 32.2
ST   D2S1338: 25
ST   D3S1358: 15,16
ST   D5S818: 12
ST   D7S820: 9,12
ST   D8S1179: 13
ST   FGA: 19.2,24
ST   Penta D: 8
ST   Penta E: 5,12
ST   TH01: 9.3
ST   TPOX: 8
ST   vWA: 17,18
DI   NCIt; C9325; Adrenal cortex carcinoma
DI   ORDO; Orphanet_1501; Adrenocortical carcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0456 ! NCI-H295
SX   Female
AG   48Y
CA   Cancer cell line
DT   Created: 04-04-12; Last updated: 27-11-25; Version: 31
CH   CVCL_5V09 ! HAC13
CH   CVCL_S898 ! HAC15
CH   CVCL_5V08 ! HAC50
CH   CVCL_F1SA ! HyCyte NCI-H295R KO-hNPC1
//
RX   PubMed=22266826; DOI=10.1055/s-0031-1298019; PMCID=PMC4269336;
RA   Wang, Tao
RA   Rowland, Jennifer G.
RA   Parmar, Jeniel
RA   Nesterova, Maria
RA   Seki, Tsugio
RA   Rainey, William E.
RT   "Comparison of aldosterone production among human adrenocortical cell
RT   lines.";
RL   Horm. Metab. Res. 44:245-250(2012).
//
RX   PubMed=35563746; DOI=10.3390/cells11091439; PMCID=PMC9103477;
RA   Sigala, Sandra
RA   Bothou, Christina
RA   Penton, David
RA   Abate, Andrea
RA   Peitzsch, Mirko
RA   Cosentini, Deborah
RA   Tiberio, Guido Alberto Massimo
RA   Bornstein, Stefan Richard
RA   Berruti, Alfredo
RA   Hantel, Constanze
RT   "A comprehensive investigation of steroidogenic signaling in classical
RT   and new experimental cell models of adrenocortical carcinoma.";
RL   Cells 11:1439.1-1439.17(2022).
//
RX   PubMed=35764904; DOI=10.1007/s12020-022-03112-w; PMCID=PMC9385758;
RA   Sigala, Sandra
RA   Rossini, Elisa
RA   Abate, Andrea
RA   Tamburello, Mariangela
RA   Bornstein, Stefan Richard
RA   Hantel, Constanze
RT   "An update on adrenocortical cell lines of human origin.";
RL   Endocrine 77:432-437(2022).
//
RX   PubMed=29371329; DOI=10.1530/ERC-17-0447; PMCID=PMC5831504;
RA   Kiseljak-Vassiliades, Katja
RA   Zhang, Yu
RA   Bagby, Stacey M.
RA   Kar, Adwitiya
RA   Pozdeyev, Nikita
RA   Xu, Mei
RA   Gowan, Katherine
RA   Sharma, Vibha
RA   Raeburn, Christopher D.
RA   Albuja-Cruz, Maria Belen
RA   Jones, Kenneth L.
RA   Fishbein, Lauren
RA   Schweppe, Rebecca E.
RA   Somerset, Hilary
RA   Pitts, Todd M.
RA   Leong, Stephen
RA   Wierman, Margaret E.
RT   "Development of new preclinical models to advance adrenocortical
RT   carcinoma research.";
RL   Endocr. Relat. Cancer 25:437-451(2018).
//
RX   PubMed=34572026; DOI=10.3390/cells10092376; PMCID=PMC8466536;
RA   Wellman, Kimberly
RA   Fu, Rui
RA   Baldwin, Amber
RA   Rege, Juilee
RA   Murphy, Elisabeth
RA   Rainey, William E.
RA   Mukherjee, Neelanjan
RT   "Transcriptomic response dynamics of human primary and immortalized
RT   adrenocortical cells to steroidogenic stimuli.";
RL   Cells 10:2376.1-2376.15(2021).
//
RX   PubMed=15541570; DOI=10.1016/j.mce.2003.12.020;
RA   Rainey, William E.
RA   Saner, Karla
RA   Schimmer, Bernard P.
RT   "Adrenocortical cell lines.";
RL   Mol. Cell. Endocrinol. 228:23-38(2004).
//
RX   PubMed=33981288; DOI=10.3389/fendo.2021.669426; PMCID=PMC8108132;
RA   Rossini, Elisa
RA   Tamburello, Mariangela
RA   Abate, Andrea
RA   Beretta, Silvia
RA   Fragni, Martina
RA   Cominelli, Manuela
RA   Cosentini, Deborah
RA   Hantel, Constanze
RA   Bono, Federica
RA   Grisanti, Salvatore
RA   Poliani, Pietro Luigi
RA   Tiberio, Guido Alberto Massimo
RA   Memo, Maurizio
RA   Sigala, Sandra
RA   Berruti, Alfredo
RT   "Cytotoxic effect of progesterone, tamoxifen and their combination in
RT   experimental cell models of human adrenocortical cancer.";
RL   Front. Endocrinol. 12:669426.1-669426.13(2021).
//
RX   PubMed=21924324; DOI=10.1016/j.mce.2011.08.041; PMCID=PMC3288152;
RA   Wang, Tao
RA   Rainey, William E.
RT   "Human adrenocortical carcinoma cell lines.";
RL   Mol. Cell. Endocrinol. 351:58-65(2012).
//
RX   PubMed=18000308; DOI=10.1677/JOE-07-0166;
RA   Samandari, Elika
RA   Kempna, Petra
RA   Nuoffer, Jean-Marc
RA   Hofer, Gaby
RA   Mullis, Primus-Eugen
RA   Fluck, Christa E.
RT   "Human adrenal corticocarcinoma NCI-H295R cells produce more androgens
RT   than NCI-H295A cells and differ in 3beta-hydroxysteroid dehydrogenase
RT   type 2 and 17,20 lyase activities.";
RL   J. Endocrinol. 195:459-472(2007).
//
RX   PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662;
RA   Rothenberg, S. Michael
RA   Mohapatra, Gayatry
RA   Rivera, Miguel N.
RA   Winokur, Daniel
RA   Greninger, Patricia
RA   Nitta, Mai
RA   Sadow, Peter M.
RA   Sooriyakumar, Gaya
RA   Brannigan, Brian W.
RA   Ulman, Matthew J.
RA   Perera, Rushika M.
RA   Wang, Rui
RA   Tam, Angela
RA   Ma, Xiao-Jun
RA   Erlander, Mark
RA   Sgroi, Dennis C.
RA   Rocco, James W.
RA   Lingen, Mark W.
RA   Cohen, Ezra E.W.
RA   Louis, David Neil
RA   Settleman, Jeffrey
RA   Haber, Daniel Arie
RT   "A genome-wide screen for microdeletions reveals disruption of
RT   polarity complex genes in diverse human cancers.";
RL   Cancer Res. 70:2158-2164(2010).
//
RX   PubMed=23951072; DOI=10.1371/journal.pone.0071022; PMCID=PMC3738630;
RA   Nishi, Haruhisa
RA   Arai, Hirokazu
RA   Momiyama, Toshihiko
RT   "NCI-H295R, a human adrenal cortex-derived cell line, expresses
RT   purinergic receptors linked to Ca(2)(+)-mobilization/influx and
RT   cortisol secretion.";
RL   PLoS ONE 8:e71022.1-e71022.13(2013).
//