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Cellosaurus Ku812 (CVCL_0379)

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Cell line name Ku812
Synonyms KU812; KU-812; KU.812; KU 812
Accession CVCL_0379
Resource Identification Initiative To cite this cell line use: Ku812 (RRID:CVCL_0379)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Part of: LL-100 blood cancer cell line panel.
Population: Japanese.
Doubling time: 25 +- 2 hours (PubMed=11264164); ~20-30 hours (ATCC=CRL-2099); ~80-100 hours (DSMZ=ACC-378).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Phenotyping; Drug screening.
Omics: Proteomics; Quantitative.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; SNP array analysis.
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Cell type: Basophil progenitor cell; CL=CL_0000613.
Sequence variations
  • Gene fusion; HGNC; HGNC:76; ABL1 + HGNC; HGNC:1014; BCR; Name(s)=BCR-ABL1, BCR-ABL; Note=BCR exon 14 fused to ABL1 exon 2 (b3a2 transcript) (PubMed=10071072; PubMed=10576511; PubMed=15843827; PubMed=20809971; DepMap=ACH-000074).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Lys132Arg (c.395A>G); ClinVar=VCV000376625; Zygosity=Homozygous (Cosmic-CLP=907311).
HLA typing Source: PubMed=26589293
Class I
HLA-AA*24:02,26:01
HLA-BB*40:02,40:02
HLA-CC*03:04,04:01
Class II
HLA-DQDQA1*03:02,03:02
DQB1*02:02,06:04
HLA-DRDRB1*10:01,14:05

Source: DSMZCellDive=ACC-378
Class I
HLA-AA*24:02:01,26:01:01
HLA-BB*40:01:02,40:02:01
HLA-CC*03:04:01,04:82
Genome ancestry Source: PubMed=30894373

Origin% genome
African0
Native American0.62
East Asian, North85.34
East Asian, South12.57
South Asian0.16
European, North0.88
European, South0.42
Disease Chronic myelogenous leukemia, BCR-ABL1 positive (NCIt: C3174)
Chronic myeloid leukemia (ORDO: Orphanet_521)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_E6I5 (KU812 IM1)CVCL_E6I6 (KU812 IM2)CVCL_E6I7 (KU812 IM3)
CVCL_0615 (Ku812E)CVCL_0616 (Ku812F)
Sex of cell Male
Age at sampling 38Y
Category Cancer cell line
STR profile Source(s): ATCC=CRL-2099; Cosmic-CLP=907311; DSMZ=ACC-378; ECACC=90071807; JCRB=IFO50363; JCRB=JCRB0104; PubMed=11416159; PubMed=25877200; RCB=RCB0495

Markers:
AmelogeninX,Y
CSF1PO12,13
D2S133818
D3S135815
D5S81810
D7S82012
D8S117911,15
D13S3178
D16S5399
D18S5113
D19S43313,14
D21S1130,31.2
FGA22,24
Penta D12
Penta E5,19
TH019
TPOX12
vWA15

Run an STR similarity search on this cell line
Publications

PubMed=3858609; DOI=10.1016/0145-2126(85)90060-8
Kenji Kishi;
A new leukemia cell line with Philadelphia chromosome characterized as basophil precursors.
Leuk. Res. 9:381-390(1985)

PubMed=3332852; DOI=10.1016/S0950-3536(87)80037-9
Armand Keating;
Ph positive CML cell lines.
Baillieres Clin. Haematol. 1:1021-1029(1987)

PubMed=2540861; DOI=10.1182/blood.V73.7.2003.2003
Masaki Nakazawa, Maria-Teresa Mitjavila-Garcia, Najet Debili, Nicole Casadevall, Patrick Mayeux, Philippe Rouyer-Fessard, Anne Dubart-Kupperschmitt, Paul-Henri Romeo, Yves Beuzard, Kenji Kishi, Janine Breton-Gorius, William Vainchenker;
KU 812: a pluripotent human cell line with spontaneous erythroid terminal maturation.
Blood 73:2003-2013(1989)

DOI=10.11418/jtca1981.15.4_211
Yoshinobu Matsuo, Ayumi Okochi, Toshio Ariyasu, Emi Iimura, Tadao Ohno;
Identification of cell lines with variable numbers of tandem repeat (VNTR) amplified by polymerase chain reaction.
Tissue Cult. Res. Commun. 15:211-219(1996)

PubMed=9290701; DOI=10.1002/(SICI)1098-2744(199708)19:4<243::AID-MC5>3.0.CO;2-D
Li-Qun Jia, Motonobu Osada, Chikashi Ishioka, Makio Gamo, Shuntaro Ikawa, Takao Suzuki, Hideki Shimodaira, Tomohito Niitani, Toshio Kudo, Mitoshi Akiyama, Narimiti Kimura ...Show all 18 authors... , Mitsuyoshi Matsuo, Hiroshi Mizusawa, Noriho Tanaka, Hideki Koyama, Masayoshi Namba, Ryunosuke Kanamaru, Toshio Kuroki; Show fewer authors
Screening the p53 status of human cell lines using a yeast functional assay.
Mol. Carcinog. 19:243-253(1997)

PubMed=9510473; DOI=10.1111/j.1349-7006.1998.tb00476.x; PMCID=PMC5921588
Noriko Hosoya, Akira Hangaishi, Seishi Ogawa, Kiyoshi Miyagawa, Kinuko Mitani, Yoshio Yazaki, Hisamaru Hirai;
Frameshift mutations of the hMSH6 gene in human leukemia cell lines.
Jpn. J. Cancer Res. 89:33-39(1998)

PubMed=9644295; DOI=10.1159/000040837
Hiroto Kaneko, Shigeo Horiike, Yuri Sasai, Toshiki Iwai, Makoto Nakao, Shouhei Yokota, Masafumi Taniwaki, Kei Kashima, Shin-ichi Misawa;
Rare alteration of genomic structure or expression of the DPC4 gene in myelogenous leukemias.
Acta Haematol. 99:187-190(1998)

PubMed=10071072; DOI=10.1016/S0145-2126(98)00171-4
Hans Gunther Drexler, Roderick A.F. MacLeod, Cord C. Uphoff;
Leukemia cell lines: in vitro models for the study of Philadelphia chromosome-positive leukemia.
Leuk. Res. 23:207-215(1999)

PubMed=10576511; DOI=10.1016/S0145-2126(99)00131-9
Cord C. Uphoff, Silke Habig, Severine Fombonne, Yoshinobu Matsuo, Hans Gunther Drexler;
ABL-BCR expression in BCR-ABL-positive human leukemia cell lines.
Leuk. Res. 23:1055-1060(1999)

DOI=10.1016/B978-0-12-221970-2.50457-5
Hans Gunther Drexler;
The leukemia-lymphoma cell line factsbook.
(In book) ISBN 9780122219702; pp.1-733; Academic Press; London; United Kingdom (2001)

PubMed=11264164; DOI=10.1182/blood.V97.7.1999
Yasuhiko Kano, Miyuki Akutsu, Saburo Tsunoda, Hiroyuki Mano, Yuko Sato, Yoshio Honma, Yusuke Furukawa;
In vitro cytotoxic effects of a tyrosine kinase inhibitor STI571 in combination with commonly used antileukemic agents.
Blood 97:1999-2007(2001)

PubMed=11416159; DOI=10.1073/pnas.121616198; PMCID=PMC35459
John R.W. Masters, Jim A. Thomson, Bernadette Daly-Burns, Yvonne A. Reid, Wilhelm Gerhard Dirks, Phil Packer, Lorraine Hellenga Toji, Tadao Ohno, Hideyuki Tanabe, Colin Francis Arlett, Lloyd R. Kelland ...Show all 16 authors... , Maureen Harrison, Arvind K. Virmani, Timothy H. Ward, Karen L. Ayres, Paul G. Debenham; Show fewer authors
Short tandem repeat profiling provides an international reference standard for human cell lines.
Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001)

PubMed=15843827; DOI=10.1038/sj.leu.2403749
Anna K. Hagstrom-Andersson, Patrik Eden, David Lindgren, Jens Nilsson, Carin Lassen, Jesper Heldrup, Magnus Fontes, Ake Borg, Felix Mitelman, Bertil Johansson, Mattias Hoglund, Thoas Fioretos;
Gene expression profiling of leukemic cell lines reveals conserved molecular signatures among subtypes with specific genetic aberrations.
Leukemia 19:1042-1050(2005)

PubMed=16408098; DOI=10.1038/sj.leu.2404081
Hilmar Quentmeier, Roderick A.F. MacLeod, Margarete Zaborski, Hans Gunther Drexler;
JAK2 V617F tyrosine kinase mutation in cell lines derived from myeloproliferative disorders.
Leukemia 20:471-476(2006)

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113
Graham Robert Bignell, Christopher D. Greenman, Helen R. Davies, Adam P. Butler, Sarah Edkins, Jenny M. Andrews, Gemma Buck, Lina Chen, David Beare, Calli Latimer, Sara Widaa ...Show all 22 authors... , Jonathan Hinton, Ciara Fahey, Bei-Yuan Fu, Sajani Swamy, Gillian L. Dalgliesh, Bin Tean Teh, Panos Deloukas, Feng-Tang Yang, Peter J. Campbell, P. Andrew Futreal, Michael Rudolf Stratton; Show fewer authors
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662
S. Michael Rothenberg, Gayatry Mohapatra, Miguel N. Rivera, Daniel Winokur, Patricia Greninger, Mai Nitta, Peter M. Sadow, Gaya Sooriyakumar, Brian W. Brannigan, Matthew J. Ulman, Rushika M. Perera ...Show all 22 authors... , Rui Wang, Angela Tam, Xiao-Jun Ma, Mark Erlander, Dennis C. Sgroi, James W. Rocco, Mark W. Lingen, Ezra E.W. Cohen, David Neil Louis, Jeffrey Settleman, Daniel Arie Haber; Show fewer authors
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=20809971; DOI=10.1186/1755-8166-3-15; PMCID=PMC2944125
Anna Virgili, Elisabeth Nacheva;
Genomic amplification of BCR/ABL1 and a region downstream of ABL1 in chronic myeloid leukaemia: a FISH mapping study of CML patients and cell lines.
Mol. Cytogenet. 3:15.1-15.12(2010)

PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027
Jordi Ginesta Barretina, Giordano Caponigro, Nicolas Stransky, Kavitha Venkatesan, Adam A. Margolin, Sungjoon Kim, Christopher J. Wilson, Joseph Lehar, Gregory V. Kryukov, Dmitriy Sonkin, Anupama Reddy ...Show all 61 authors... , Manway Liu, Lauren Murray, Michael F. Berger, John E. Monahan, Paula Morais, Jodi Meltzer, Adam Korejwa, Judit Jane-Valbuena, Felipa A. Mapa, Joseph Thibault, Eva Bric-Furlong, Pichai Raman, Aaron Shipway, Ingo H. Engels, Jill Cheng, Guo-Ying K. Yu, Jian-Jun Yu, Peter Aspesi Jr., Melanie de Silva, Kalpana Jagtap, Michael D. Jones, Li Wang, Charles Hatton, Emanuele Palescandolo, Supriya Gupta, Scott Mahan, Carrie Sougnez, Robert C. Onofrio, Ted Liefeld, Laura Eleanor MacConaill, Wendy Winckler, Michael Reich, Nan-Xin Li, Jill P. Mesirov, Stacey B. Gabriel, Gad Getz, Kristin Ardlie, Vivien Chan, Vic E. Myer, Barbara L. Weber, Jeff Porter, Markus Warmuth, Peter Finan, Jennifer L. Harris, Matthew Langer Meyerson, Todd Robert Golub, Michael P. Morrissey, William Raj Sellers, Robert Schlegel, Levi Alexander Garraway; Show fewer authors
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=25149487; DOI=10.1007/978-1-4939-1173-8_8
Egle Passante;
Mast cell and basophil cell lines: a compendium.
Methods Mol. Biol. 1192:101-113(2014)

PubMed=25485619; DOI=10.1038/nbt.3080
Christiaan Klijn, Steffen Durinck, Eric W. Stawiski, Peter M. Haverty, Zhao-Shi Jiang, Han-Bin Liu, Jeremiah Degenhardt, Oleg Mayba, Florian Gnad, Jin-Feng Liu, Gregoire Pau ...Show all 30 authors... , Jens Reeder, Yi Cao, Kiran Mukhyala, Suresh K. Selvaraj, Ma-Mie Yu, Gregory J. Zynda, Matthew J. Brauer, Thomas D. Wu, Robert Clifford Gentleman, Gerard Manning, Robert L. Yauch, Richard Bourgon, David Stokoe, Zora Modrusan, Richard M. Neve, Frederic J. de Sauvage, Jeffrey Settleman, Somasekar Seshagiri, Ze-Min Zhang; Show fewer authors
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25877200; DOI=10.1038/nature14397
Ma-Mie Yu, Suresh K. Selvaraj, May M.Y. Liang-Chu, Sahar Aghajani, Matthew Busse, Jean Yuan, Genee Lee, Franklin V. Peale, Christiaan Klijn, Richard Bourgon, Joshua S. Kaminker, Richard M. Neve;
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878
Jelle Scholtalbers, Sebastian Boegel, Thomas Bukur, Marius Byl, Sebastian Goerges, Patrick Sorn, Martin Loewer, Ugur Sahin, John C. Castle;
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Francesco Iorio, Theo A. Knijnenburg, Daniel J. Vis, Graham Robert Bignell, Michael Patrick Menden, Michael Schubert, Nanne Aben, Emanuel Goncalves, Syd Barthorpe, Howard Lightfoot, Thomas Cokelaer ...Show all 39 authors... , Patricia Greninger, Ewald van Dyk, Han Chang, Heshani de Silva, Holger Heyn, Xian-Ming Deng, Regina K. Egan, Qing-Song Liu, Tatiana Mironenko, Xeni Mitropoulos, Laura Richardson, Jin-Hua Wang, Ting-Hu Zhang, Sebastian Moran, Sergi Sayols, Maryam Soleimani, David Tamborero, Nuria Lopez-Bigas, Petra Ross-Macdonald, Manel Esteller, Nathanael S. Gray, Daniel Arie Haber, Michael Rudolf Stratton, Cyril Henri Benes, Lodewyk F.A. Wessels, Julio Saez-Rodriguez, Ultan McDermott, Mathew J. Garnett; Show fewer authors
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=30285677; DOI=10.1186/s12885-018-4840-5; PMCID=PMC6167786
Kar-Tong Tan, Ling-Wen Ding, Qiao-Yang Sun, Zhen-Tang Lao, Wenwen Chien, Xi Ren, Jin-Fen Xiao, Xin-Yi Loh, Liang Xu, Michael Lill, Anand Mayakonda ...Show all 14 authors... , De-Chen Lin, Henry He Yang, Harold Phillip Koeffler; Show fewer authors
Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines.
BMC Cancer 18:940.1-940.13(2018)

PubMed=30629668; DOI=10.1371/journal.pone.0210404; PMCID=PMC6328144
Cord C. Uphoff, Claudia Pommerenke, Sabine A. Denkmann, Hans Gunther Drexler;
Screening human cell lines for viral infections applying RNA-Seq data analysis.
PLoS ONE 14:e0210404.1-e0210404.22(2019)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Mahmoud Ghandi, Franklin W. Huang, Judit Jane-Valbuena, Gregory V. Kryukov, Christopher C. Lo, E. Robert McDonald 3rd, Jordi Ginesta Barretina, Ellen T. Gelfand, Craig M. Bielski, Hao-Xin Li, Kevin Hu ...Show all 68 authors... , Alexander Y. Andreev-Drakhlin, Jaegil Kim, Julian M. Hess, Brian J. Haas, Francois Aguet, Barbara A. Weir, Michael V. Rothberg, Brenton R. Paolella, Michael Scott Lawrence, Rehan Akbani, Yi-Ling Lu, Hong L. Tiv, Prafulla C. Gokhale, Antoine de Weck, Ali Amin Mansour, Coyin Oh, Juliann Shih, Kevin Hadi, Yanay Rosen, Jonathan Bistline, Kavitha Venkatesan, Anupama Reddy, Dmitriy Sonkin, Manway Liu, Joseph Lehar, Joshua M. Korn, Dale A. Porter, Michael D. Jones, Javad Golji, Giordano Caponigro, Jordan E. Taylor, Caitlin M. Dunning, Amanda L. Creech, Allison C. Warren, James M. McFarland, Mahdi Zamanighomi, Audrey Kauffmann, Nicolas Stransky, Marcin Imielinski, Yosef E. Maruvka, Andrew D. Cherniack, Aviad Tsherniak, Francisca Vazquez, Jacob D. Jaffe, Andrew Alan Lane, David M. Weinstock, Cory M. Johannessen, Michael P. Morrissey, Frank Stegmeier, Robert Schlegel, William Chun Hahn, Gad Getz, Gordon B. Mills, Jesse S. Boehm, Todd Robert Golub, Levi Alexander Garraway, William Raj Sellers; Show fewer authors
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=31160637; DOI=10.1038/s41598-019-44491-x; PMCID=PMC6547646
Hilmar Quentmeier, Claudia Pommerenke, Wilhelm Gerhard Dirks, Sonja Eberth, Max Koeppel, Roderick A.F. MacLeod, Stefan Nagel, Klaus G. Steube, Cord C. Uphoff, Hans Gunther Drexler;
The LL-100 panel: 100 cell lines for blood cancer studies.
Sci. Rep. 9:8218.1-8218.14(2019)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Emanuel Goncalves, Rebecca C. Poulos, Zhao-Xiang Cai, Syd Barthorpe, Srikanth S. Manda, Natasha Lucas, Alexandra Beck, Daniel Bucio-Noble, Michael Dausmann, Caitlin Hall, Michael Hecker ...Show all 32 authors... , Jennifer Koh, Howard Lightfoot, Sadia Mahboob, Iman Mali, James Morris, Laura Richardson, Akila J. Seneviratne, Rebecca Shepherd, Erin Sykes, Frances Thomas, Sara Valentini, Steven G. Williams, Yang-Xiu Wu, Dylan Xavier, Karen L. MacKenzie, Peter G. Hains, Brett Tully, Phillip James Robinson, Qing Zhong, Mathew J. Garnett, Roger Robert Reddel; Show fewer authors
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line collections (Providers) ATCC; CRL-2099
BCRC; 60502
BCRJ; 0261
DSMZ; ACC-378
ECACC; 90071807
JCRB; IFO50363
JCRB; JCRB0104
RCB; RCB0495
Cell line databases/resources CLO; CLO_0007124
CLO; CLO_0007125
CLO; CLO_0050989
MCCL; MCC:0000274
CLDB; cl3070
cancercelllines; CVCL_0379
CCRID; 1101HUM-PUMC000522
CCRID; 3101HUMTCHu189
Cell_Model_Passport; SIDM00308
CGH-DB; 9211-4
Cosmic-CLP; 907311
DepMap; ACH-000074
DSMZCellDive; ACC-378
IGRhCellID; KU812
LINCS_LDP; LCL-1106
Lonza; 1207
Anatomy/cell type resources BTO; BTO_0000732
Biological sample resources BioSample; SAMN01821693
BioSample; SAMN01821733
BioSample; SAMN03472893
BioSample; SAMN10988528
Chemistry resources ChEMBL-Cells; CHEMBL3308504
ChEMBL-Targets; CHEMBL613997
GDSC; 907311
PharmacoDB; KU812_795_2019
PubChem_Cell_line; CVCL_0379
Encyclopedic resources Wikidata; Q54900693
Experimental variables resources EFO; EFO_0002222
Gene expression databases ArrayExpress; E-MTAB-38
ArrayExpress; E-MTAB-2706
ArrayExpress; E-MTAB-2770
ArrayExpress; E-MTAB-3610
ArrayExpress; E-MTAB-7721
ArrayExpress; E-MTAB-7722
FANTOM5_SSTAR; 10409-106B4
GEO; GSM887244
GEO; GSM888318
GEO; GSM1374606
GEO; GSM1670011
Polymorphism and mutation databases Cosmic; 787498
Cosmic; 907311
Cosmic; 919119
Cosmic; 922163
Cosmic; 924052
Cosmic; 949096
Cosmic; 991560
Cosmic; 999778
Cosmic; 1012080
Cosmic; 1026568
Cosmic; 1067444
Cosmic; 1078716
Cosmic; 1519164
Cosmic; 2009518
IARC_TP53; 342
IARC_TP53; 21446
LiGeA; CCLE_172
Progenetix; CVCL_0379
Proteomic databases PRIDE; PXD030304
Sequence databases EGA; EGAS00001000610
EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number45