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Cellosaurus ARPE-19 (CVCL_0145)

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Cell line name ARPE-19
Synonyms ARPE19; Adult Retinal Pigment Epithelial cell line-19; NTC-200; NTC200
Accession CVCL_0145
Resource Identification Initiative To cite this cell line use: ARPE-19 (RRID:CVCL_0145)
Comments Doubling time: 55 hours (Note=From cell counting), 65 hours (Note=From absorbance) (DOI=10.5897/IJBMBR2013.0154).
Omics: Metabolomics.
Omics: Proteomics.
Omics: Proteomics; 2DE-MS.
Omics: Proteomics; PTM; Phosphorylation; Quantitative.
Omics: Transcriptomics; Microarray.
Anecdotal: The number 19 in the cell line name is due to the fact that this cell line was established from a 19-year-old male who died from head trauma in a motor vehicle accident.
Misspelling: APRE-19; Note=Occasionally.
Derived from site: In situ; Eye, retina, retinal pigment epithelium; UBERON=UBERON_0001782.
Cell type: Retinal pigment epithelial cell; CL=CL_0002586.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_C8UR (ARPE-19-Luc)CVCL_IQ60 (ARPE-19/CNTF)CVCL_IQ61 (ARPE-19/EGFP)
CVCL_IQ62 (ARPE-19/GDNF)CVCL_6338 (ARPE-19/HPV-16)CVCL_E1J6 (HyCyte ARPE-19 KO-hCYP4V2)
CVCL_E1J7 (HyCyte ARPE-19 KO-hDDIT4)CVCL_YP10 (LEPI)CVCL_DF66 (NGC-0295)
CVCL_DF67 (NGC-0301)CVCL_DF68 (NGC-0363)CVCL_DF65 (NGC0211)
CVCL_D8DY (Ubigene ARPE-19 ABCA1 KO)CVCL_D8DZ (Ubigene ARPE-19 LYPLA2 KO)CVCL_D8E0 (Ubigene ARPE-19 TMEM214 KO)
CVCL_D8E1 (Ubigene ARPE-19 ZNF644 KO)
Sex of cell Male
Age at sampling 19Y
Category Spontaneously immortalized cell line
STR profile Source(s): ATCC=CRL-2302; CLS=305025; PubMed=27499609; PubMed=31412689

Markers:
AmelogeninX,Y
CSF1PO11
D2S133819
D3S135814,15
D5S81813
D6S104317,19
D7S8209,11
D8S117913
D12S39121,22
D13S31711,12
D16S5399,11
D18S5112,16
D19S43312,13
D21S1128,29
FGA23
Penta D11,13
Penta E7,11
TH016,9.3
TPOX9,11
vWA16,19

Run an STR similarity search on this cell line
Web pages Info; LRI; -; https://web.archive.org/web/20100714222125/http://www.lerner.ccf.org/eye/crabb/research/gel_arpe19_cell.php
Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/a/cell-lines-detail-617.html
Publications

PubMed=8698076; DOI=10.1006/exer.1996.0020
Kerrin Courtney Dunn, Amy E. Aotaki-Keen, Frances R. Putkey, Leonard Martin Hjelmeland;
ARPE-19, a human retinal pigment epithelial cell line with differentiated properties.
Exp. Eye Res. 62:155-169(1996)

PubMed=11504951; DOI=10.1073/pnas.171266298; PMCID=PMC55557
Raymond Douglas Lund, Peter Adamson, Yves Sauve, David J. Keegan, Sergej V. Girman, Shao-Mei Wang, Helen L. Winton, Naheed Kanuga, Anthony S.L. Kwan, Laurence Beauchene, Anne Zerbib ...Show all 15 authors... , Len Hetherington, Pierre-Olivier Couraud, Peter J. Coffey, John Greenwood; Show fewer authors
Subretinal transplantation of genetically modified human cell lines attenuates loss of visual function in dystrophic rats.
Proc. Natl. Acad. Sci. U.S.A. 98:9942-9947(2001)

PubMed=14551534; PMCID=PMC2819117
Anna T. Rogojina, William E. Orr, Bong K. Song, Eldon E. Geisert Jr.;
Comparing the use of Affymetrix to spotted oligonucleotide microarrays using two retinal pigment epithelium cell lines.
Mol. Vis. 9:482-496(2003)

PubMed=16262907; DOI=10.1186/1471-2415-5-25; PMCID=PMC1291373
Rajesh K. Sharma, William E. Orr, Anthony D. Schmitt, David A. Johnson;
A functional profile of gene expression in ARPE-19 cells.
BMC Ophthalmol. 5:25.1-25.10(2005)

PubMed=21697133; DOI=10.1167/iovs.11-7479
Mio Oshikawa, Chihiro Tsutsui, Tomoko Ikegami, Yuki Fuchida, Maki Matsubara, Shigeru Toyama, Ron Usami, Kuniyo Ohtoko, Seishi Kato;
Full-length transcriptome analysis of human retina-derived cell lines ARPE-19 and Y79 using the vector-capping method.
Invest. Ophthalmol. Vis. Sci. 52:6662-6670(2011)

DOI=10.5897/IJBMBR2013.0154
Simon Bernard Iloki Assanga, Armida Andrea Gil-Salido, Lydianis Maria Lewis Lujan, Aaron de Jesus Rosas-Durazo, Ana Lilian Acosta-Silva, Elba Griselda Rivera-Castaneda, Jose Luis Rubio-Pino;
Cell growth curves for different cell lines and their relationship with biological activities.
Int. J. Biotechnol. Mol. Biol. Res. 4:60-70(2013)

PubMed=24251032; DOI=10.1155/2013/216359; PMCID=PMC3819763
Lara Pasovic, Tor Paaske Utheim, Rima Maria, Torstein Lyberg, Edward B. Messelt, Peder Aabel, Dong-Feng Chen, Xiang-Jun Chen, Jon Roger Eidet;
Optimization of storage temperature for cultured ARPE-19 cells.
J. Ophthalmol. 2013:216359.1-216359.11(2013)

PubMed=25177495; DOI=10.1155/2014/801787; PMCID=PMC4142280
Alla V. Kuznetsova, Alexander M. Kurinov, Maria A. Aleksandrova;
Cell models to study regulation of cell transformation in pathologies of retinal pigment epithelium.
J. Ophthalmol. 2014:801787.1-801787.18(2014)

PubMed=27499609; PMCID=PMC4961466
Ying Wang, Ai-Min Sang, Man-Hui Zhu, Guo-Wei Zhang, Huai-Jin Guan, Min Ji, Hui Chen;
Tissue factor induces VEGF expression via activation of the Wnt/beta-catenin signaling pathway in ARPE-19 cells.
Mol. Vis. 22:886-897(2016)

PubMed=28978645; DOI=10.1074/jbc.M117.812677; PMCID=PMC5712622
Cheng-Kang Chiang, Aleksander Tworak, Brian M. Kevany, Bo Xu, Janice Mayne, Zhi-Bin Ning, Daniel Figeys, Krzysztof Palczewski;
Quantitative phosphoproteomics reveals involvement of multiple signaling pathways in early phagocytosis by the retinal pigmented epithelium.
J. Biol. Chem. 292:19826-19839(2017)

PubMed=29476476; DOI=10.1007/978-1-4939-7680-5_17
Elizaveta Fasler-Kan, Nijas Aliu, Kerstin Wunderlich, Sylvia Ketterer, Sabrina Ruggiero, Steffen Berger, Peter Meyer;
The retinal pigment epithelial cell line (ARPE-19) displays mosaic structural chromosomal aberrations.
Methods Mol. Biol. 1745:305-314(2018)

PubMed=30167441; DOI=10.1016/j.dib.2018.06.103; PMCID=PMC6111057
Diwa Koirala, Sarka Beranova-Giorgianni, Francesco Giorgianni;
Data-independent proteome analysis of ARPE-19 cells.
Data Brief 20:333-336(2018)

PubMed=31254428; DOI=10.1111/ceo.13578
Rachel Churm, Gareth J. Dunseath, Sarah L. Prior, Rebecca L. Thomas, Sanjiv Banerjee, David R. Owens;
Development and characterization of an in vitro system of the human retina using cultured cell lines.
Clin. Exp. Ophthalmol. 47:1055-1062(2019)

PubMed=31412689; DOI=10.3390/pharmaceutics11080412; PMCID=PMC6722654
Laura Hellinen, Lea Pirskanen, Unni Tengvall-Unadike, Arto Urtti, Mika Reinisalo;
Retinal pigment epithelial cell line with fast differentiation and improved barrier properties.
Pharmaceutics 11:412.1-412.12(2019)

Cross-references
Cell line collections (Providers) ATCC; CRL-2302
BCRC; 60383
BCRJ; 0041
CCTCC; GDC0323
CLS; 305025
IZSLER; BS CL 193
Ubigene; YC-C033
Cell line databases/resources CLO; CLO_0001749
MCCL; MCC:0000038
CCRID; 3101HUMGNHu45
CCRID; 4201HUM-CCTCC00323
Lonza; 871
Anatomy/cell type resources BTO; BTO_0002335
Biological sample resources BioSample; SAMN03471862
ENCODE; ENCBS054LTE
ENCODE; ENCBS534TJM
Chemistry resources ChEMBL-Cells; CHEMBL4295388
ChEMBL-Targets; CHEMBL4296399
PubChem_Cell_line; CVCL_0145
Encyclopedic resources Wikidata; Q28812659
Experimental variables resources EFO; EFO_0006271
Gene expression databases GEO; GSM1227434
Metabolomic databases MetaboLights; MTBLS213
Proteomic databases PRIDE; PXD006125
PRIDE; PXD006269
PRIDE; PXD009607
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number36