Cellosaurus cell line 4B6/3598/3442#2/4011C1 (CVCL

Cellosaurus 4B6/3598/3442#2/4011C1 (CVCL_ZC43)

[Text version]

Cell line name

4B6/3598/3442#2/4011C1

Accession

CVCL_ZC43

Resource Identification Initiative

To cite this cell line use: 4B6/3598/3442#2/4011C1 (RRID:CVCL_ZC43)

Comments

Characteristics: Unlike the parental cell line, this cell line maintains mtDNA at normal levels. This was achieved by introducing into the parental cell line a ligA gene fused to an excisable (FRT/FLPo) mitochondrial targeting sequence (MTS) from human ornithine transcarbamylase. The resulting cell line expresses two ligA variants: one without- and another with MTS. The latter is efficiently imported into mitochondria and therefore normal mtDNA copy number is restored in the transduced cells (Kerafast=ESA104).
Characteristics: Transfected with reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16.
Knockout cell: Method=KO mouse; MGI; MGI:109152; Lig3.
Genetic integration: Method=Transduction; Gene=UniProtKB; P03070; Simian virus 40 (SV40) large T antigen.
Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transfection; Gene=UniProtKB; P33967; Bacillus cereus Bsr.
Genetic integration: Method=Transfection; Gene=UniProtKB; P15042; Escherichia coli ligA.
Genetic integration: Method=Transfection; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Genetic integration: Method=Transfection; Gene=UniProtKB; P06492; Human herpesvirus 1 (HHV-1) VP16 (UL28) (Note=With p.Met1_Arg360del).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Genetic integration: Method=Transfection; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Genetic integration: Method=Transgenic mouse; Gene=MGI; MGI:1352467; Esr1 (Note=Ligand binding domain; with p.Gly525Arg. Fused to Cre = CreERT2).
Genetic integration: Method=Transgenic mouse; Gene=UniProtKB; P06956; Escherichia phage P1 cre (Note=Fused to ligand domain of mouse Esr1 = CreERT2).
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
Derived from site: In situ; Embryo; UBERON=UBERON_0000922.
Cell type: Fibroblast; CL=CL_0000057.

Species of origin

Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: B6.Cg-Tg(CAG-cre/Esr1)5Amc/J x Lig3LoxP.

Hierarchy

Parent: CVCL_ZC42 (4B6/3598/3442#2)
Children:

CVCL_ZC44 (4B6/3598/3442#2/4011/4014C1-2)

Sex of cell

Sex unspecified

Age at sampling

13-14FD