Cellosaurus 4B6/3598/3442#2/4011/4014C1-2 (CVCL_ZC44)
| Cell line name | 4B6/3598/3442#2/4011/4014C1-2 |
|---|---|
| Accession | CVCL_ZC44 |
| Resource Identification Initiative | To cite this cell line use: 4B6/3598/3442#2/4011/4014C1-2 (RRID:CVCL_ZC44) |
| Comments | Characteristics: To generate this cell line the parental cells were transiently transfected with a plasmid pMA4014, which encodes mCherry and FLPo and flow sorted for transfected cells. As a result of FLPo-mediated excision of the MTS in front of the bacterial ligA gene, mitochondrial import of ligA in this cell line is inefficient and mtDNA is maintained at a reduced copy number (Kerafast=ESA105). Characteristics: Transfected with reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16. Knockout cell: Method=KO mouse; MGI; MGI:109152; Lig3. Genetic integration: Method=Transduction; Gene=UniProtKB; P03070; SV40 large T antigen. Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=Transfection; Gene=UniProtKB; P33967; B.cereus Bsr. Genetic integration: Method=Transfection; Gene=UniProtKB; P15042; Escherichia coli ligA. Genetic integration: Method=Transfection; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR. Genetic integration: Method=Transfection; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del). Genetic integration: Method=Transfection; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo. Genetic integration: Method=Transgenic mouse; Gene=MGI; MGI:1352467; Esr1 (Note=Ligand binding domain; with p.Gly525Arg. Fused to Cre = CreERT2). Genetic integration: Method=Transgenic mouse; Gene=UniProtKB; P06956; Escherichia phage P1 cre (Note=Fused to ligand domain of mouse Esr1 = CreERT2). Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40). Derived from site: In situ; Embryo; UBERON=UBERON_0000922. Cell type: Fibroblast; CL=CL_0000057. |
| Species of origin | Mus musculus (Mouse)
(NCBI Taxonomy: 10090)
Breed/subspecies: B6.Cg-Tg(CAG-cre/Esr1)5Amc/J x Lig3LoxP. |
| Hierarchy | Parent: CVCL_ZC43 (4B6/3598/3442#2/4011C1) |
| Sex of cell | Sex unspecified |
| Age at sampling | 13-14FD |
| Category | Transformed cell line |
| Publications | PubMed=23884459; DOI=10.1074/jbc.M113.472977; PMCID=PMC3772206 |
| Cross-references | |
| Cell line collections (Providers) | Kerafast; ESA105 |
| Encyclopedic resources | Wikidata; Q98125415 |
| Entry history | |
| Entry creation | 02-Jul-2020 |
| Last entry update | 10-Apr-2025 |
| Version number | 11 |