ID   4B6/3598/3442#2/4011C1
AC   CVCL_ZC43
DR   Kerafast; ESA104
DR   Wikidata; Q98125419
RX   PubMed=23884459;
CC   Characteristics: Unlike the parental cell line, this cell line maintains mtDNA at normal levels. This was achieved by introducing into the parental cell line a ligA gene fused to an excisable (FRT/FLPo) mitochondrial targeting sequence (MTS) from human ornithine transcarbamylase. The resulting cell line expresses two ligA variants: one without- and another with MTS. The latter is efficiently imported into mitochondria and therefore normal mtDNA copy number is restored in the transduced cells (Kerafast=ESA104).
CC   Characteristics: Transfected with reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16.
CC   Knockout cell: Method=KO mouse; MGI; MGI:109152; Lig3.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P03070; SV40 large T antigen.
CC   Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P33967; B.cereus Bsr.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P15042; Escherichia coli ligA.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Genetic integration: Method=Transgenic mouse; Gene=MGI; MGI:1352467; Esr1 (Note=Ligand binding domain; with p.Gly525Arg. Fused to Cre = CreERT2).
CC   Genetic integration: Method=Transgenic mouse; Gene=UniProtKB; P06956; Escherichia phage P1 cre (Note=Fused to ligand domain of mouse Esr1 = CreERT2).
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
CC   Derived from site: In situ; Embryo; UBERON=UBERON_0000922.
CC   Cell type: Fibroblast; CL=CL_0000057.
CC   Breed/subspecies: B6.Cg-Tg(CAG-cre/Esr1)5Amc/J x Lig3LoxP.
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_ZC42 ! 4B6/3598/3442#2
SX   Sex unspecified
AG   13-14FD
CA   Transformed cell line
DT   Created: 02-07-20; Last updated: 10-04-25; Version: 11
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RX   PubMed=23884459; DOI=10.1074/jbc.M113.472977; PMCID=PMC3772206;
RA   Shokolenko I.N., Fayzulin R.Z., Katyal S., McKinnon P.J., Wilson G.L.,
RA   Alexeyev M.F.;
RT   "Mitochondrial DNA ligase is dispensable for the viability of cultured
RT   cells but essential for mtDNA maintenance.";
RL   J. Biol. Chem. 288:26594-26605(2013).
//