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Cellosaurus Aag-2 (CVCL_Z617)

[Text version]
Cell line name Aag-2
Synonyms Aag2; Peleg's line 59; 59; AA-P
Accession CVCL_Z617
Resource Identification Initiative To cite this cell line use: Aag-2 (RRID:CVCL_Z617)
Comments Group: Insect cell line.
Doubling time: ~24 hours (PubMed=627872).
Omics: Genomics; Whole genome sequencing.
Omics: Transcriptomics; RNAseq.
Species of origin Aedes aegypti (Yellowfever mosquito) (Culex aegypti) (NCBI Taxonomy: 7159)
Hierarchy Children:
CVCL_C3S1 (Aag2 (C))CVCL_C0RB (Aag2 eGFP-Ago3)CVCL_C0RC (Aag2 eGFP-Piwi4)
CVCL_C0RD (Aag2 eGFP-Piwi5)CVCL_C0RE (Aag2 eGFP-Piwi6)CVCL_A8M5 (Aag2-AF5)
CVCL_B6Y7 (Aag2INF)
Sex of cell Sex unspecified
Age at sampling Neonate larva
Category Spontaneously immortalized cell line
Web pages Info; VectorBase; -; https://vectorbase.org/vectorbase/app/record/dataset/DS_72b2b87ee3
Info; VectorBase; -; https://vectorbase.org/vectorbase/app/record/dataset/DS_dba68c637d
Info; VectorBase; -; https://vectorbase.org/vectorbase/app/record/dataset/DS_f6bb097de7
Provider; TCB; -; https://www.liverpool.ac.uk/research/facilities/tick-cell-biobank/
Publications

PubMed=4869110; DOI=10.4269/ajtmh.1968.17.219
Joseph Peleg;
Growth of arboviruses in primary tissue culture of Aedes aegypti embryos.
Am. J. Trop. Med. Hyg. 17:219-223(1968)

PubMed=5142332; DOI=10.1007/978-3-642-65224-0_1
Imogene Schneider;
The culture of cells from insects and ticks. I. Cultivation of dipteran cells in vitro.
Curr. Top. Microbiol. Immunol. 55:1-12(1971)

DOI=10.1303/aez.13.170
Jun Mitsuhashi;
Amino acid requirements of some continuous cell lines of insects.
Appl. Entomol. Zool. 13:170-175(1978)

PubMed=627872; DOI=10.1099/0022-1317-38-2-231
Joseph Peleg, Marit Pecht;
Adaptation of an Aedes aegypti mosquito cell line to growth at 15 degrees C and its response to infection by Sindbis virus.
J. Gen. Virol. 38:231-239(1978)

CLPUB00492
Jun Mitsuhashi;
Nutritional requirements of insect cells in vitro.
(In book chapter) Invertebrate cell system applications, Vol. I; Mitsuhashi, Jun (eds.); pp.3-20; CRC Press; Boca Raton; USA (1989)

PubMed=2267971; DOI=10.4269/ajtmh.1990.43.669
Que Lan, Ann M. Fallon;
Small heat shock proteins distinguish between two mosquito species and confirm identity of their cell lines.
Am. J. Trop. Med. Hyg. 43:669-676(1990)

PubMed=22827926; DOI=10.1186/1756-3305-5-148; PMCID=PMC3419682
Ana Beatriz Ferreira Barletta, Maria Clara L. Nascimento Silva, Marcos H. Ferreira Sorgine;
Validation of Aedes aegypti Aag-2 cells as a model for insect immune studies.
Parasit. Vectors 5:148.1-148.9(2012)

PubMed=28497119; DOI=10.1128/mSphere.00144-17; PMCID=PMC5415634
Margus Varjak, Kevin Maringer, Mick Watson, Vattipally B. Sreenu, Anthony C. Fredericks, Emilie Pondeville, Claire L. Donald, Jelle Sterk, Joy Kean, Marie Vazeille, Anna-Bella Failloux ...Show all 13 authors... , Alain Kohl, Esther Schnettler; Show fewer authors
Aedes aegypti Piwi4 is a noncanonical PIWI protein involved in antiviral responses.
mSphere 2:e00144.17.1-e00144.17.16(2017)

PubMed=32807211; DOI=10.1186/s13071-020-04294-w; PMCID=PMC7433057
Man-Jin Li, Ce-Jie Lan, He-Ting Gao, Dan Xing, Zhen-Yu Gu, Duo Su, Tong-Yan Zhao, Hui-Ying Yang, Chun-Xiao Li;
Transcriptome analysis of Aedes aegypti Aag2 cells in response to dengue virus-2 infection.
Parasit. Vectors 13:421.1-421.14(2020)

PubMed=34878970; DOI=10.1099/jgv.0.001700
Athos Silva de Oliveira, Anna Fernanda Vasconcellos, Bruno Milhomem Pilati Rodrigues, Leonardo Assis da Silva, Renato Oliveira Resende, Bergmann Morais Ribeiro;
Chikungunya virus produced by a persistently infected mosquito cell line comprises a shorter genome and is non-infectious to mammalian cells.
J. Gen. Virol. 102:001700.1-001700.11(2021)

Cross-references
Anatomy/cell type resources BTO; BTO_0006292
Encyclopedic resources Wikidata; Q54607594
Proteomic databases PRIDE; PXD010191
PRIDE; PXD033231
Entry history
Entry creation10-Apr-2015
Last entry update10-Apr-2025
Version number19