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Cellosaurus WAe009-A-20 (CVCL_WJ42)

[Text version]
Cell line name WAe009-A-20
Synonyms VIBCBDe010-A-20
Accession CVCL_WJ42
Resource Identification Initiative To cite this cell line use: WAe009-A-20 (RRID:CVCL_WJ42)
Comments From: VIB - KU Leuven Center for Brain and Disease Research; Leuven; Belgium.
Characteristics: Using homologous recombination a doxycycline inducible dCAS9-VPR (VPR is a tripartite activation system made of a fusion of VP64, p65(relA), and RTA) along with GFP and rtTA driven by CAGS promoter was introduced in the AAVS1 safe harbor locus.
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (with p.Asp10Ala and p.His840Ala = dCas9).
Transfected with: MGI:103290; Rela (with p.Met1_Leu368 del).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_9773 (WA09)
Sex of cell Female
Age at sampling Blastocyst stage
Category Embryonic stem cell
Cell line databases/resources hPSCreg; WAe009-A-20
Biological sample resources BioSamples; SAMEA5728497
Encyclopedic resources Wikidata; Q98134740
Entry history
Entry creation24-May-2019
Last entry update30-Jan-2024
Version number6