ID   WAe009-A-20
AC   CVCL_WJ42
SY   VIBCBDe010-A-20
DR   BioSamples; SAMEA5728497
DR   hPSCreg; WAe009-A-20
DR   Wikidata; Q98134740
CC   From: VIB - KU Leuven Center for Brain and Disease Research; Leuven; Belgium.
CC   Characteristics: Using homologous recombination a doxycycline inducible dCAS9-VPR (VPR is a tripartite activation system made of a fusion of VP64, p65(relA), and RTA) along with GFP and rtTA driven by CAGS promoter was introduced in the AAVS1 safe harbor locus.
CC   Genetic integration: Method=Homologous recombination; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Homologous recombination; Gene=MGI; MGI:103290; Rela (Note=With p.Met1_Leu368del).
CC   Genetic integration: Method=Homologous recombination; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala = dCas9).
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_9773 ! WA09
SX   Female
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 24-05-19; Last updated: 10-04-25; Version: 9
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