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Cellosaurus N2a/Gary (CVCL_WI08)

[Text version]
Cell line name N2a/Gary
Accession CVCL_WI08
Resource Identification Initiative To cite this cell line use: N2a/Gary (RRID:CVCL_WI08)
Comments Group: Patented cell line.
Registration: International Depositary Authority, European Collection of Cell Cultures (ECACC); 02031337.
Biotechnology: Used in the scrapie cell (SC) assay. This susceptible cell line is exposed to mouse prion-containing samples for 3 days, grown to confluence and split 1:10 three times. The proportion of PrPSc-containing cells is determined with automated counting equipment.
Characteristics: Highly susceptible to infection by prion (PrPsc).
Derived from site: In situ; Peripheral nervous system; UBERON=UBERON_0000010.
Cell type: Neural crest derived neuroblast; CL=CL_0002676.
Disease Mouse neuroblastoma (NCIt: C21993)
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: A/J.
Hierarchy Parent: CVCL_0470 (Neuro-2a)
CVCL_WI09 (N2a-PD88)
Sex of cell Male
Category Cancer cell line

Enari M., Klohn P.-C., Flechsig E., Stoltze L., Weissmann C.
Method for the detection of prion proteins.
Patent number WO2003081249A2, 02-Oct-2003

PubMed=14504404; DOI=10.1073/pnas.1834432100
Klohn P.-C., Stoltze L., Flechsig E., Enari M., Weissmann C.
A quantitative, highly sensitive cell-based infectivity assay for mouse scrapie prions.
Proc. Natl. Acad. Sci. U.S.A. 100:11666-11671(2003)

Encyclopedic resources Wikidata; Q95991907
Entry history
Entry creation24-May-2019
Last entry update29-Jun-2023
Version number6