ID   N2a/Gary
AC   CVCL_WI08
DR   Wikidata; Q95991907
RX   Patent=WO2003081249A2;
RX   PubMed=14504404;
CC   Group: Patented cell line.
CC   Registration: International Depositary Authority, European Collection of Cell Cultures (ECACC); 02031337.
CC   Biotechnology: Used in the scrapie cell (SC) assay. This susceptible cell line is exposed to mouse prion-containing samples for 3 days, grown to confluence and split 1:10 three times. The proportion of PrPSc-containing cells is determined with automated counting equipment.
CC   Characteristics: Highly susceptible to infection by prion (PrPsc).
CC   Derived from site: In situ; Peripheral nervous system; UBERON=UBERON_0000010.
CC   Cell type: Neural crest derived neuroblast; CL=CL_0002676.
CC   Breed/subspecies: A/J.
DI   NCIt; C21993; Mouse neuroblastoma
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_0470 ! Neuro-2a
SX   Male
CA   Cancer cell line
DT   Created: 24-05-19; Last updated: 29-06-23; Version: 6
//
RX   Patent=WO2003081249A2;
RA   Enari M., Klohn P.-C., Flechsig E., Stoltze L., Weissmann C.;
RT   "Method for the detection of prion proteins.";
RL   Patent number WO2003081249A2, 02-Oct-2003.
//
RX   PubMed=14504404; DOI=10.1073/pnas.1834432100;
RA   Klohn P.-C., Stoltze L., Flechsig E., Enari M., Weissmann C.;
RT   "A quantitative, highly sensitive cell-based infectivity assay for
RT   mouse scrapie prions.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:11666-11671(2003).
//