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Cellosaurus U2OS#18 (CVCL_VR75)

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Cell line name U2OS#18
Synonyms U2OS clone #18; U2OS reporter cell line #18
Accession CVCL_VR75
Resource Identification Initiative To cite this cell line use: U2OS#18 (RRID:CVCL_VR75)
Comments Population: Caucasian.
Characteristics: Contains a 'nested intron' reporter construct HRsub that allows to study sister chromatid recombination (SCR) by positive selection of 'long tract' gene conversion events between sister chromatids.
Doubling time: ~50-60 hours (DSMZ=ACC-832).
Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transfection; Gene=UniProtKB; P33967; Bacillus cereus Bsr.
Genetic integration: Method=Transfection; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
Sequence variations
  • Mutation; HGNC; HGNC:9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
Disease Osteosarcoma (NCIt: C9145)
Osteosarcoma (ORDO: Orphanet_668)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0042 (U2OS)
Children:
CVCL_VR78 (U2OS#18-RAD51B-8)CVCL_VR80 (U2OS#18-RAD51C-15)CVCL_VR82 (U2OS#18-RAD51D-4)
CVCL_VR84 (U2OS#18-XRCC2-5E)CVCL_VR86 (U2OS#18-XRCC3-6A)
Sex of cell Female
Age at sampling 15Y
Category Cancer cell line
STR profile Source(s): DSMZ=ACC-832

Markers:
AmelogeninX
CSF1PO12,13
D2S133820,24
D3S135816
D5S8188,11
D7S82011,12
D8S117912,14
D13S31713
D16S53911,12
D18S5112,14
D19S43315
D21S1131,32
FGA20
Penta D9
Penta E10,13
TH016,9.3
TPOX11,12
vWA14,18

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Publications

PubMed=15590323; DOI=10.1016/j.dnarep.2004.08.010; PMCID=PMC2967438
Nadine Puget, Melodie Knowlton, Ralph Scully;
Molecular analysis of sister chromatid recombination in mammalian cells.
DNA Repair 4:149-161(2005)

Cross-references
Cell line collections (Providers) DSMZ; ACC-832
Cell line databases/resources DSMZCellDive; ACC-832
Encyclopedic resources Wikidata; Q98133776
Entry history
Entry creation25-Feb-2019
Last entry update14-Aug-2025
Version number13