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Cellosaurus U2OS#18-RAD51C-15 (CVCL_VR80)

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Cell line name U2OS#18-RAD51C-15
Accession CVCL_VR80
Resource Identification Initiative To cite this cell line use: U2OS#18-RAD51C-15 (RRID:CVCL_VR80)
Comments Population: Caucasian.
Characteristics: Contains a 'nested intron' reporter construct HRsub that allows to study sister chromatid recombination (SCR) by positive selection of 'long tract' gene conversion events between sister chromatids.
Doubling time: ~50-60 hours (DSMZ=ACC-834).
Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:9820; RAD51C.
Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transfection; Gene=UniProtKB; P33967; Bacillus cereus Bsr.
Genetic integration: Method=Transfection; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
Sequence variations
  • Mutation; HGNC; HGNC:9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
Disease Osteosarcoma (NCIt: C9145)
Osteosarcoma (ORDO: Orphanet_668)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_VR75 (U2OS#18)
Children:
CVCL_VR81 (U2OS#18-RAD51C-15/C+)
Sex of cell Female
Age at sampling 15Y
Category Cancer cell line
STR profile Source(s): DSMZ=ACC-834

Markers:
AmelogeninX
CSF1PO13
D2S133820,24
D3S135816
D5S8188,11
D7S82011,12
D8S117912,14
D13S31713
D16S53911,12
D18S5112,14
D19S43315
D21S1131
FGA20
Penta D9
Penta E10,13
TH016,9.3
TPOX11,12
vWA14,18

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Cross-references
Cell line collections (Providers) DSMZ; ACC-834
Cell line databases/resources DSMZCellDive; ACC-834
Encyclopedic resources Wikidata; Q98133779
Entry history
Entry creation25-Feb-2019
Last entry update14-Aug-2025
Version number13