ID   U2OS#18
AC   CVCL_VR75
SY   U2OS clone #18; U2OS reporter cell line #18
DR   DSMZ; ACC-832
DR   DSMZCellDive; ACC-832
DR   Wikidata; Q98133776
RX   PubMed=15590323;
CC   Population: Caucasian.
CC   Characteristics: Contains a 'nested intron' reporter construct HRsub that allows to study sister chromatid recombination (SCR) by positive selection of 'long tract' gene conversion events between sister chromatids.
CC   Doubling time: ~50-60 hours (DSMZ=ACC-832).
CC   Sequence variation: Mutation; HGNC; HGNC:9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
CC   Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P33967; B.cereus Bsr.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
ST   Source(s): DSMZ=ACC-832
ST   Amelogenin: X
ST   CSF1PO: 12,13
ST   D13S317: 13
ST   D16S539: 11,12
ST   D18S51: 12,14
ST   D19S433: 15
ST   D21S11: 31,32
ST   D2S1338: 20,24
ST   D3S1358: 16
ST   D5S818: 8,11
ST   D7S820: 11,12
ST   D8S1179: 12,14
ST   FGA: 20
ST   Penta D: 9
ST   Penta E: 10,13
ST   TH01: 6,9.3
ST   TPOX: 11,12
ST   vWA: 14,18
DI   NCIt; C9145; Osteosarcoma
DI   ORDO; Orphanet_668; Osteosarcoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0042 ! U2OS
SX   Female
AG   15Y
CA   Cancer cell line
DT   Created: 25-02-19; Last updated: 19-12-24; Version: 12
//
RX   PubMed=15590323; DOI=10.1016/j.dnarep.2004.08.010; PMCID=PMC2967438;
RA   Puget N., Knowlton M., Scully R.;
RT   "Molecular analysis of sister chromatid recombination in mammalian
RT   cells.";
RL   DNA Repair 4:149-161(2005).
//