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Cellosaurus HT-1080/Arabidopsis hybrid chr15 (CVCL_VI98)

[Text version]
Cell line name HT-1080/Arabidopsis hybrid chr15
Accession CVCL_VI98
Resource Identification Initiative To cite this cell line use: HT-1080/Arabidopsis hybrid chr15 (RRID:CVCL_VI98)
Comments Characteristics: Hybrid human cell containing an Arabidopsis chromosome translocated on hum chr15.
Characteristics: Arabidopsis cells were transformed using two constructs, one containing EGFP under the control of the CAG promoter and the other containing Bsd under the control of the human pGK promoter. The CAG and pGK promoter have high activity only in human cells, resulting in the CC strong EGFP expression only after the plant chromosome carrying marker genes was transferred into human cells.
Transfected with: UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd).
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Arabidopsis thaliana (Mouse-ear cress) (NCBI Taxonomy: 3702)
Hierarchy Parent: CVCL_0317 (HT-1080)
Category Hybrid cell line

PubMed=27696824; DOI=10.1021/acssynbio.6b00180
Wada N., Kazuki Y., Kazuki K., Inoue T., Fukui K., Oshimura M.
Maintenance and function of a plant chromosome in human cells.
ACS Synth. Biol. 6:301-310(2017)

PubMed=29754235; DOI=10.1007/978-1-4939-7795-6_16
Wada N., Kazuki Y., Kazuki K., Inoue T., Fukui K., Oshimura M.
Production of a human cell line with a plant chromosome.
Methods Mol. Biol. 1772:289-296(2018)

PubMed=33785802; DOI=10.1038/s41598-021-86130-4
Liu Y.-K., Liaw Y.M., Teo C.H., Capal P., Wada N., Fukui K., Dolezel J., Ohmido N.
Molecular organization of recombinant human-Arabidopsis chromosomes in hybrid cell lines.
Sci. Rep. 11:7160-7160(2021)

Encyclopedic resources Wikidata; Q94304258
Entry history
Entry creation07-Sep-2018
Last entry update21-Mar-2023
Version number4