ID   HT-1080/Arabidopsis hybrid chr15
AC   CVCL_VI98
DR   Wikidata; Q94304258
RX   PubMed=27696824;
RX   PubMed=29754235;
RX   PubMed=33785802;
CC   Characteristics: Hybrid human cell containing an Arabidopsis chromosome translocated on hum chr15.
CC   Characteristics: Arabidopsis cells were transformed using two constructs, one containing EGFP under the control of the CAG promoter and the other containing Bsd under the control of the human pGK promoter. The CAG and pGK promoter have high activity only in human cells, resulting in the CC strong EGFP expression only after the plant chromosome carrying marker genes was transferred into human cells.
CC   Transfected with: UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd).
CC   Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
OX   NCBI_TaxID=3702; ! Arabidopsis thaliana (Mouse-ear cress)
HI   CVCL_0317 ! HT-1080
CA   Hybrid cell line
DT   Created: 07-09-18; Last updated: 21-03-23; Version: 4
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RX   PubMed=27696824; DOI=10.1021/acssynbio.6b00180;
RA   Wada N., Kazuki Y., Kazuki K., Inoue T., Fukui K., Oshimura M.;
RT   "Maintenance and function of a plant chromosome in human cells.";
RL   ACS Synth. Biol. 6:301-310(2017).
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RX   PubMed=29754235; DOI=10.1007/978-1-4939-7795-6_16;
RA   Wada N., Kazuki Y., Kazuki K., Inoue T., Fukui K., Oshimura M.;
RT   "Production of a human cell line with a plant chromosome.";
RL   Methods Mol. Biol. 1772:289-296(2018).
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RX   PubMed=33785802; DOI=10.1038/s41598-021-86130-4;
RA   Liu Y.-K., Liaw Y.M., Teo C.H., Capal P., Wada N., Fukui K., Dolezel J.,
RA   Ohmido N.;
RT   "Molecular organization of recombinant human-Arabidopsis chromosomes
RT   in hybrid cell lines.";
RL   Sci. Rep. 11:7160-7160(2021).
//