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Cellosaurus HUDEP-2 (CVCL_VI06)

[Text version]
Cell line name HUDEP-2
Synonyms HUDEP2; Human Umbilical cord blood-Derived Erythroid Progenitor-2
Accession CVCL_VI06
Resource Identification Initiative To cite this cell line use: HUDEP-2 (RRID:CVCL_VI06)
Comments Population: Japanese.
Characteristics: After in vitro induction of differentiation, will produce enucleated red blood cells.
Characteristics: Expression of the HPV16 E6 and E7 genes is induced by tetracycline (PubMed=23533656).
Doubling time: 22.7 +- 5.0 hours (PubMed=31753923).
Genetic integration: Method=Transduction; Gene=UniProtKB; P03126; Human papillomavirus type 16 protein E6.
Genetic integration: Method=Transduction; Gene=UniProtKB; P03129; Human papillomavirus type 16 protein E7.
Transformant: NCBI_TaxID; 333760; Human papillomavirus type 16 (HPV16).
Omics: Genomics; Chromatin accessibility; ATAC-seq.
Omics: Proteomics; Quantitative.
Omics: Transcriptomics; RNAseq.
Miscellaneous: Please contact directly Dr. Nakamura Y. for the use of this cell line (yukionak@brc.riken.jp).
Derived from site: In situ; Umbilical cord blood; UBERON=UBERON_0012168.
Cell type: Erythroid progenitor cell; CL=CL_0000038.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_C0QM (HUDEP2 HBB-KO)CVCL_YT59 (ssHUDEP2)
Sex of cell Sex unspecified
Age at sampling <1D
Category Transformed cell line
Publications

PubMed=23533656; DOI=10.1371/journal.pone.0059890; PMCID=PMC3606290
Ryo Kurita, Noriko Suda, Kazuhiro Sudo, Kenichi Miharada, Takashi Hiroyama, Hiroyuki Miyoshi, Kenzaburo Tani, Yukio Nakamura;
Establishment of immortalized human erythroid progenitor cell lines able to produce enucleated red blood cells.
PLoS ONE 8:e59890.1-e59890.15(2013)

Patent=US8975072
Yukio Nakamura, Ryo Kurita;
Human erythroid progenitor cell line comprising HPV E6/E7 operably linked to an inducible promoter and method for producing human enucleated red blood cells.
Patent number US8975072, 10-Mar-2015

PubMed=31164570; DOI=10.3390/mps1030028; PMCID=PMC6481050
Gemma Moir-Meyer, Pak Leng Cheong, Aude-Anais Olijnik, Jill Brown, Samantha Knight, Andrew King, Ryo Kurita, Yukio Nakamura, Richard J. Gibbons, Douglas Roland Higgs, Veronica J. Buckle, Christian Babbs;
Robust CRISPR/Cas9 genome editing of the HUDEP-2 erythroid precursor line using plasmids and single-stranded oligonucleotide donors.
Methods Protoc. 1:28.1-28.17(2018)

PubMed=31753923; DOI=10.3324/haematol.2019.229211; PMCID=PMC7395286
Deborah E. Daniels, Damien J. Downes, Ivan Ferrer-Vicens, Daniel C.J. Ferguson, Belinda K. Singleton, Marieangela C. Wilson, Kongtana Trakarnsanga, Ryo Kurita, Yukio Nakamura, David John Anstee, Jan Frayne;
Comparing the two leading erythroid lines BEL-A and HUDEP-2.
Haematologica 105:e389-e394(2020)

PubMed=35269949; DOI=10.3390/ijms23052807; PMCID=PMC8911073
Guo-Qiang Zhou, Hao-Kun Zhang, An-Ning Lin, Zhen Wu, Ting Li, Xu-Min Zhang, Hong-Yan Chen, Da-Ru Lu;
Multi-omics analysis in beta-thalassemia using an HBB gene-knockout human erythroid progenitor cell model.
Int. J. Mol. Sci. 23:2807.1-2807.14(2022)

Cross-references
Cell line collections (Providers) RCB; RCB4557
CRISP screens repositories BioGRID_ORCS_Cell_line; 1800
Encyclopedic resources Wikidata; Q54896730
Experimental variables resources EFO; EFO_0022838
Proteomic databases PRIDE; PXD030182
Entry history
Entry creation14-May-2018
Last entry update10-Apr-2025
Version number16