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Cellosaurus HCLE (CVCL_UZ28)

[Text version]
Cell line name HCLE
Synonyms HuCl-22/cdk4R/p53DD/TERT
Accession CVCL_UZ28
Resource Identification Initiative To cite this cell line use: HCLE (RRID:CVCL_UZ28)
Comments Genetic integration: Method=Transduction; Gene=HGNC; HGNC:1773; CDK4 (Note=With p.Arg24Cys).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT.
Genetic integration: Method=Transduction; Gene=MGI; MGI:98834; Trp53 (Note=With p.Leu14_Lys302del = p53DD).
Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Derived from site: In situ; Eye, cornea, limbus; UBERON=UBERON_0006761.
Cell type: Keratinocyte; CL=CL_0000312.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_UZ26 (HuCL-22)
Sex of cell Male
Age at sampling 30Y
Category Telomerase immortalized cell line
STR profile Source(s): PubMed=29787296

Markers:
AmelogeninX,Y
CSF1PO12,13
D3S135815,16
D5S81811,12
D7S82010,11
D8S117911,13
D13S31710,12
D16S53911,13
D18S5114,16
D21S1131
FGA19,24
Penta D11,16
Penta E7
TH016,9
TPOX8
vWA16,17

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Web pages Provider; BWH dermatology; -; https://dermatology.bwh.harvard.edu/docs/Cell-Culture-Core-Cell-Lines.pdf
Publications

PubMed=12077343; DOI=10.1128/mcb.22.14.5157-5172.2002; PMCID=PMC139780
James George Rheinwald, William Chun Hahn, Matthew Robert Ramsey, Jenny Y. Wu, Zong-You Guo, Hensin Tsao, Michele De Luca, Caterina Catricala, Kathleen M. O'Toole;
A two-stage, p16(INK4A)- and p53-dependent keratinocyte senescence mechanism that limits replicative potential independent of telomere status.
Mol. Cell. Biol. 22:5157-5172(2002)

PubMed=12766048; DOI=10.1167/iovs.02-0851
Ilene K. Gipson, Sandra Spurr-Michaud, Pablo Argueso, Ann Tisdale, Tat Fong Ng, Cindy Leigh Russo;
Mucin gene expression in immortalized human corneal-limbal and conjunctival epithelial cell lines.
Invest. Ophthalmol. Vis. Sci. 44:2496-2506(2003)

PubMed=29787296; DOI=10.1080/02713683.2018.1480043; PMCID=PMC6590053
Alison M. McDermott, Hasna Baidouri, Ashley M. Woodward, Wendy R. Kam, Yang Liu, Xiao-Min Chen, Jillian F. Ziemanski, Kerry Vistisen, Linda D. Hazlett, Kelly K. Nichols, Pablo Argueso, David A. Sullivan;
Short tandem repeat (STR) profiles of commonly used human ocular surface cell lines.
Curr. Eye Res. 43:1097-1101(2018)

Cross-references
Encyclopedic resources Wikidata; Q93984540
Entry history
Entry creation25-Feb-2019
Last entry update14-Aug-2025
Version number13