ID   HCLE
AC   CVCL_UZ28
SY   HuCl-22/cdk4R/p53DD/TERT
DR   Wikidata; Q93984540
RX   PubMed=12077343;
RX   PubMed=12766048;
RX   PubMed=29787296;
WW   Provider; BWH dermatology; -; https://dermatology.bwh.harvard.edu/docs/Cell-Culture-Core-Cell-Lines.pdf
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:1773; CDK4 (Note=With p.Arg24Cys).
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT.
CC   Genetic integration: Method=Transduction; Gene=MGI; MGI:98834; Trp53 (Note=With p.Leu14_Lys302del = p53DD).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Derived from site: In situ; Eye, cornea, limbus; UBERON=UBERON_0006761.
CC   Cell type: Keratinocyte; CL=CL_0000312.
ST   Source(s): PubMed=29787296
ST   Amelogenin: X,Y
ST   CSF1PO: 12,13
ST   D13S317: 10,12
ST   D16S539: 11,13
ST   D18S51: 14,16
ST   D21S11: 31
ST   D3S1358: 15,16
ST   D5S818: 11,12
ST   D7S820: 10,11
ST   D8S1179: 11,13
ST   FGA: 19,24
ST   Penta D: 11,16
ST   Penta E: 7
ST   TH01: 6,9
ST   TPOX: 8
ST   vWA: 16,17
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_UZ26 ! HuCL-22
SX   Male
AG   30Y
CA   Telomerase immortalized cell line
DT   Created: 25-02-19; Last updated: 10-04-25; Version: 12
//
RX   PubMed=12077343; DOI=10.1128/mcb.22.14.5157-5172.2002; PMCID=PMC139780;
RA   Rheinwald J.G., Hahn W.C., Ramsey M.R., Wu J.Y., Guo Z.-Y., Tsao H.,
RA   De Luca M., Catricala C., O'Toole K.M.;
RT   "A two-stage, p16(INK4A)- and p53-dependent keratinocyte senescence
RT   mechanism that limits replicative potential independent of telomere
RT   status.";
RL   Mol. Cell. Biol. 22:5157-5172(2002).
//
RX   PubMed=12766048; DOI=10.1167/iovs.02-0851;
RA   Gipson I.K., Spurr-Michaud S., Argueso P., Tisdale A., Ng T.F.,
RA   Russo C.L.;
RT   "Mucin gene expression in immortalized human corneal-limbal and
RT   conjunctival epithelial cell lines.";
RL   Invest. Ophthalmol. Vis. Sci. 44:2496-2506(2003).
//
RX   PubMed=29787296; DOI=10.1080/02713683.2018.1480043; PMCID=PMC6590053;
RA   McDermott A.M., Baidouri H., Woodward A.M., Kam W.R., Liu Y., Chen X.-M.,
RA   Ziemanski J.F., Vistisen K., Hazlett L.D., Nichols K.K., Argueso P.,
RA   Sullivan D.A.;
RT   "Short tandem repeat (STR) profiles of commonly used human ocular
RT   surface cell lines.";
RL   Curr. Eye Res. 43:1097-1101(2018).
//