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Cellosaurus LFBK-alphaVbeta6 (CVCL_RX27)

[Text version]
Cell line name LFBK-alphaVbeta6
Synonyms LFBK alphaVbeta6; LFBKalphaVbeta6; LF-BK alphaVbeta6; LFBK-alpha(v)beta(6); LFBK-aVb6; LFBK-AvB6; LFBKaVB6; LFBKavb6
Accession CVCL_RX27
Resource Identification Initiative To cite this cell line use: LFBK-alphaVbeta6 (RRID:CVCL_RX27)
Comments Group: Patented cell line.
Problematic cell line: Misidentified. Parent cell line (LF-BK) was originally thought to be of bovine fetal kidney origin but originates from pig (PubMed=25617444). The derived cell line has also been shown to originate from pig (PubMed=34737324).
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00613.
Registration: International Depositary Authority, American Type Culture Collection (ATCC); PTA-13047.
Virology: More susceptible to infection by foot-and-mouth disease virus than parent cell line.
Virology: Susceptible to infection by senecavirus A (SVA) which can be used as a surrogate for studies of goot-and-mouth disease virus (PubMed=36146682).
Virology: Persistently infected by bovine viral diarrhea virus (BVDV) (PubMed=34092457; PubMed=34458357; PubMed=35112907).
Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Genetic integration: Method=Transduction; Gene=VGNC; VGNC:30323; Bovine ITGAV.
Genetic integration: Method=Transduction; Gene=VGNC; VGNC:30331; Bovine ITGB6.
Species of origin Sus scrofa (Pig) (NCBI Taxonomy: 9823)
Hierarchy Parent: CVCL_RX26 (LF-BK)
Children:
CVCL_A0GW (MGPK alphaVbeta6-C5)CVCL_C4XX (WRL-LFBK-alphavbeta6)
Sex of cell Sex unspecified
Age at sampling Age unspecified
Category Spontaneously immortalized cell line
Web pages Info; ICLAC; -; https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx
Publications

PubMed=23515553; DOI=10.1128/jcm.03370-12; PMCID=PMC3716081
Michael A. LaRocco, Peter W. Krug, Ed Kramer, Zaheer Ahmed, Juan M. Pacheco, Hernando Duque, Barry Baxt, Luis L. Rodriguez;
A continuous bovine kidney cell line constitutively expressing bovine alphavbeta6 integrin has increased susceptibility to foot-and-mouth disease virus.
J. Clin. Microbiol. 51:1714-1720(2013)

Patent=US9121010
Michael A. LaRocco, Barry Baxt, Hernando Duque;
Continuous porcine kidney cell line constitutively expressing bovine alphaVbeta6 integrin with increased susceptibility to foot and mouth disease virus.
Patent number US9121010, 01-Sep-2015

PubMed=25617444; DOI=10.1128/jcm.03220-14; PMCID=PMC4298512
Michael A. LaRocco, Peter W. Krug, Ed Kramer, Zaheer Ahmed, Juan M. Pacheco, Hernando Duque, Barry Baxt, Luis L. Rodriguez;
Correction: A continuous bovine kidney cell line constitutively expressing bovine alphaVbeta6 integrin has increased susceptibility to foot-and-mouth disease virus.
J. Clin. Microbiol. 53:755-755(2015)

PubMed=26082457; DOI=10.1177/1040638715584156
Katsuhiko Fukai, Kazuki Morioka, Manabu Yamada, Tatsuya Nishi, Kazuo Yoshida, Rie Kitano, Reiko Yamazoe, Toru Kanno;
Comparative performance of fetal goat tongue cell line ZZ-R 127 and fetal porcine kidney cell line LFBK-alphavbeta6 for foot-and-mouth disease virus isolation.
J. Vet. Diagn. Invest. 27:516-521(2015)

PubMed=32851014; DOI=10.3389/fvets.2020.00426; PMCID=PMC7401924
Ashley R. Gray, Britta A. Wood, Elisabeth Henry, Mehreen Azhar, Donald P. King, Valerie Mioulet;
Evaluation of cell lines for the isolation of foot-and-mouth disease virus and other viruses causing vesicular disease.
Front. Vet. Sci. 7:426.1-426.11(2020)

PubMed=34092457; DOI=10.1016/j.biologicals.2021.05.003
Michael A. LaRocco, Zaheer Ahmed, Monica Rodriguez-Calzada, Paul A. Azzinaro, Roger W. Barrette, Peter W. Krug, Luis L. Rodriguez, Teresa de Los Santos, Gisselle N. Medina;
An adventitious agent-free clonal cell line that is highly susceptible to foot -and-mouth disease virus.
Biologicals 72:33-41(2021)

PubMed=34458357; DOI=10.3389/fvets.2021.715120; PMCID=PMC8385193
Ashley R. Gray, Britta A. Wood, Elisabeth Henry, Donald P. King, Valerie Mioulet;
Elimination of non-cytopathic bovine viral diarrhea virus from the LFBK-alphavbeta6 cell line.
Front. Vet. Sci. 8:715120.1-715120.5(2021)

PubMed=34737324; DOI=10.1038/s41598-021-00779-5; PMCID=PMC8569163
Oliver Lung, Rebecca C. Candlish, Michelle Nebroski, Peter Kruckiewicz, Cody Buchanan, Mariko Moniwa;
High-throughput sequencing for species authentication and contamination detection of 63 cell lines.
Sci. Rep. 11:21657.1-21657.9(2021)

PubMed=35112907; DOI=10.1128/mra.01167-21; PMCID=PMC8812309
Lauren G. Holinka-Patterson, Ian H. Fish, Miranda R. Bertram, Ethan J. Hartwig, George R. Smoliga, Carolina Stenfeldt, Luis L. Rodriguez, Jonathan Arzt;
Genome of bovine viral diarrhea virus (BVDV) contaminating a continuous LFBK-alphaVbeta6 cell line.
Microbiol. Resour. Announc. 11:e01167.21.1-e01167.21.2(2022)

PubMed=36146682; DOI=10.3390/v14091875; PMCID=PMC9503874
Jessica Mason, Victoria Primavera, Lauren Martignette, Benjamin Clark, Jose Barrera, Janine Simmons, William Hurtle, John G. Neilan, Michael Puckette;
Comparative evaluation of the foot-and-mouth disease virus permissive LF-BK alphaVbeta6 cell line for senecavirus A research.
Viruses 14:1875.1-1875.12(2022)

Cross-references
Encyclopedic resources Wikidata; Q54902456
Entry history
Entry creation05-Mar-2018
Last entry update14-Aug-2025
Version number14