ID   LFBK-alphaVbeta6
SY   LFBK alphaVbeta6; LFBKalphaVbeta6; LF-BK alphaVbeta6; LFBK-alpha(v)beta(6); LFBK-aVb6; LFBK-AvB6; LFBKaVB6; LFBKavb6
DR   Wikidata; Q54902456
RX   Patent=US9121010;
RX   PubMed=23515553;
RX   PubMed=25617444;
RX   PubMed=26082457;
RX   PubMed=32851014;
RX   PubMed=34092457;
RX   PubMed=34458357;
RX   PubMed=34737324;
RX   PubMed=35112907;
RX   PubMed=36146682;
CC   Group: Patented cell line.
CC   Problematic cell line: Misidentified. Parent cell line (LF-BK) was originally thought to be of bovine fetal kidney origin but originates from pig (PubMed=25617444). The derived cell line has also been shown to originate from pig (PubMed=34737324).
CC   Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00613.
CC   Registration: International Depositary Authority, American Type Culture Collection (ATCC); PTA-13047.
CC   Virology: More susceptible to infection by foot-and-mouth disease virus than parent cell line.
CC   Virology: Susceptible to infection by senecavirus A (SVA) which can be used as a surrogate for studies of goot-and-mouth disease virus (PubMed=36146682).
CC   Virology: Persistently infected by bovine viral diarrhea virus (BVDV) (PubMed=34092457; PubMed=34458357; PubMed=35112907).
CC   Transfected with: VGNC; 30323; Bovine ITGAV.
CC   Transfected with: VGNC; 30331; Bovine ITGB6.
CC   Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
OX   NCBI_TaxID=9823; ! Sus scrofa (Pig)
SX   Sex unspecified
AG   Age unspecified
CA   Spontaneously immortalized cell line
DT   Created: 05-03-18; Last updated: 02-05-24; Version: 10
RX   Patent=US9121010;
RA   LaRocco M.A., Baxt B., Duque H.;
RT   "Continuous porcine kidney cell line constitutively expressing bovine
RT   alphaVbeta6 integrin with increased susceptibility to foot and mouth
RT   disease virus.";
RL   Patent number US9121010, 01-Sep-2015.
RX   PubMed=23515553; DOI=10.1128/jcm.03370-12;
RA   LaRocco M.A., Krug P.W., Kramer E., Ahmed Z., Pacheco J.M., Duque H.,
RA   Baxt B., Rodriguez L.L.;
RT   "A continuous bovine kidney cell line constitutively expressing bovine
RT   alphavbeta6 integrin has increased susceptibility to foot-and-mouth
RT   disease virus.";
RL   J. Clin. Microbiol. 51:1714-1720(2013).
RX   PubMed=25617444; DOI=10.1128/jcm.03220-14;
RA   LaRocco M.A., Krug P.W., Kramer E., Ahmed Z., Pacheco J.M., Duque H.,
RA   Baxt B., Rodriguez L.L.;
RT   "Correction: A continuous bovine kidney cell line constitutively
RT   expressing bovine alphaVbeta6 integrin has increased susceptibility to
RT   foot-and-mouth disease virus.";
RL   J. Clin. Microbiol. 53:755-755(2015).
RX   PubMed=26082457; DOI=10.1177/1040638715584156;
RA   Fukai K., Morioka K., Yamada M., Nishi T., Yoshida K., Kitano R.,
RA   Yamazoe R., Kanno T.;
RT   "Comparative performance of fetal goat tongue cell line ZZ-R 127 and
RT   fetal porcine kidney cell line LFBK-alphavbeta6 for foot-and-mouth
RT   disease virus isolation.";
RL   J. Vet. Diagn. Invest. 27:516-521(2015).
RX   PubMed=32851014; DOI=10.3389/fvets.2020.00426;
RA   Gray A.R., Wood B.A., Henry E., Azhar M., King D.P., Mioulet V.;
RT   "Evaluation of cell lines for the isolation of foot-and-mouth disease
RT   virus and other viruses causing vesicular disease.";
RL   Front. Vet. Sci. 7:426.1-426.11(2020).
RX   PubMed=34092457; DOI=10.1016/j.biologicals.2021.05.003;
RA   LaRocco M.A., Ahmed Z., Rodriguez-Calzada M., Azzinaro P.A.,
RA   Barrette R.W., Krug P.W., Rodriguez L.L., de Los Santos T.,
RA   Medina G.N.;
RT   "An adventitious agent-free clonal cell line that is highly
RT   susceptible to foot -and-mouth disease virus.";
RL   Biologicals 72:33-41(2021).
RX   PubMed=34458357; DOI=10.3389/fvets.2021.715120;
RA   Gray A.R., Wood B.A., Henry E., King D.P., Mioulet V.;
RT   "Elimination of non-cytopathic bovine viral diarrhea virus from the
RT   LFBK-alphavbeta6 cell line.";
RL   Front. Vet. Sci. 8:715120.1-715120.5(2021).
RX   PubMed=34737324; DOI=10.1038/s41598-021-00779-5;
RA   Lung O., Candlish R.C., Nebroski M., Kruckiewicz P., Buchanan C.,
RA   Moniwa M.;
RT   "High-throughput sequencing for species authentication and
RT   contamination detection of 63 cell lines.";
RL   Sci. Rep. 11:21657-21657(2021).
RX   PubMed=35112907; DOI=10.1128/mra.01167-21;
RA   Holinka-Patterson L.G., Fish I.H., Bertram M.R., Hartwig E.J.,
RA   Smoliga G.R., Stenfeldt C., Rodriguez L.L., Arzt J.;
RT   "Genome of bovine viral diarrhea virus (BVDV) contaminating a
RT   continuous LFBK-alphaVbeta6 cell line.";
RL   Microbiol. Resour. Announc. 11:e01167.21.1-e01167.21.2(2022).
RX   PubMed=36146682; DOI=10.3390/v14091875;
RA   Mason J., Primavera V., Martignette L., Clark B., Barrera J.,
RA   Simmons J., Hurtle W., Neilan J.G., Puckette M.;
RT   "Comparative evaluation of the foot-and-mouth disease virus permissive
RT   LF-BK alphaVbeta6 cell line for senecavirus A research.";
RL   Viruses 14:1875.1-1875.12(2022).