Cellosaurus cell line Nalm6-Fluc-Puro (CVCL

Cellosaurus Nalm6-Fluc-Puro (CVCL_RA36)

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Cell line name

Nalm6-Fluc-Puro

Accession

CVCL_RA36

Resource Identification Initiative

To cite this cell line use: Nalm6-Fluc-Puro (RRID:CVCL_RA36)

Comments

Population: Caucasian.
Characteristics: Reporter cell line, transduced with the LV-Fluc-P2A-PuroR vector encoding for luciferase linked via a P2A cleavage peptide to the PuroR gene and under control of the spleen focus-forming virus (SFFV) promoter (Imanis=CL151).
Genetic integration: Method=Transduction; Gene=UniProtKB; P08659; Photinus pyralis firefly-type luciferase.
Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.

Sequence variations

Gene fusion; HGNC; HGNC:50800; DUX4 + HGNC; HGNC:5477; IGH; Name(s)=IGH-DUX4; Note=The translocation occurs in the silenced IGH allele (from parent cell line).
Mutation; HGNC; HGNC:3236; EGFR; Simple; p.Arg1068Ter (c.3202C>T); Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Ala146Thr (c.436G>A); Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; HGNC:9864; RARA; Simple; p.Gln45Ter (c.133C>T); ClinVar=VCV000393019; Zygosity=Heterozygous (from parent cell line).

Disease

Adult B acute lymphoblastic leukemia (NCIt: C9143)
Precursor B-cell acute lymphoblastic leukemia (ORDO: Orphanet_99860)

Species of origin

Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy

Parent: CVCL_0092 (NALM-6)
Children:

CVCL_D5K5 (Nalm6-Fluc-Puro/CD19-KO)

CVCL_E4BT (Nalm6-Fluc-Puro/HuCD19-Puro (low))

CVCL_E4BU (Nalm6-Fluc-Puro/HuCD19-Puro (mid))

Sex of cell

Male

Age at sampling

19Y