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Cellosaurus Nalm6-Fluc-Puro/HuCD19-Puro (mid) (CVCL_E4BU)

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Cell line name Nalm6-Fluc-Puro/HuCD19-Puro (mid)
Accession CVCL_E4BU
Resource Identification Initiative To cite this cell line use: Nalm6-Fluc-Puro/HuCD19-Puro (mid) (RRID:CVCL_E4BU)
Comments Population: Caucasian.
Characteristics: Reporter cell line, transduced with the LV-Fluc-P2A-PuroR vector encoding for luciferase linked via a P2A cleavage peptide to the PuroR gene and under control of the spleen focus-forming virus (SFFV) promoter (from parent cell line).
Characteristics: Transduced with a human CD19 and PuroR encoding lentivirus. A moderate CD19 expressing population was generated by selection using a methylcellulose based semi-solid medium (Imanis=CL183).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:1633; CD19.
Genetic integration: Method=Transduction; Gene=UniProtKB; P08659; Photinus pyralis firefly-type luciferase.
Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Sequence variations
  • Gene fusion; HGNC; HGNC:50800; DUX4 + HGNC; HGNC:5477; IGH; Name(s)=IGH-DUX4; Note=The translocation occurs in the silenced IGH allele (from parent cell line).
  • Mutation; HGNC; HGNC:3236; EGFR; Simple; p.Arg1068Ter (c.3202C>T); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Ala146Thr (c.436G>A); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:9864; RARA; Simple; p.Gln45Ter (c.133C>T); ClinVar=VCV000393019; Zygosity=Heterozygous (from parent cell line).
Disease Adult B acute lymphoblastic leukemia (NCIt: C9143)
Precursor B-cell acute lymphoblastic leukemia (ORDO: Orphanet_99860)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_RA36 (Nalm6-Fluc-Puro)
Sex of cell Male
Age at sampling 19Y
Category Cancer cell line
Cross-references
Cell line collections (Providers) Imanis; CL183
Entry history
Entry creation19-Dec-2024
Last entry update14-Aug-2025
Version number2