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Cellosaurus KM20 (CVCL_L095)

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Cell line name KM20
Synonyms KM-20
Accession CVCL_L095
Resource Identification Initiative To cite this cell line use: KM20 (RRID:CVCL_L095)
Comments Problematic cell line: Contaminated. Shown to be a HT-29 derivative (ICLAC; an early sample of KM20 had been tested by the core facility where the cell line was established and the STR profile corresponds to that of HT-29).
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00513.
Population: Caucasian.
Microsatellite instability: Stable (MSS) (PubMed=25926053).
Omics: Transcriptomics; Microarray.
Derived from site: In situ; Colon; UBERON=UBERON_0001155.
Sequence variations
  • Mutation; HGNC; HGNC:583; APC; Simple; p.Glu853Ter (c.2557G>T); ClinVar=VCV001071632; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:583; APC; Simple; p.Thr1556Asnfs*3 (c.4666dupA) (c.4666_4667insA); ClinVar=VCV000428112; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.Pro449Thr (c.1345C>A); ClinVar=VCV001333250; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:6770; SMAD4; Simple; p.Gln311Ter (c.931C>T); ClinVar=VCV000230663; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg273His (c.818G>A); ClinVar=VCV000012366; Zygosity=Homozygous (from parent cell line).
Disease Colon adenocarcinoma (NCIt: C4349)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0320 (HT-29)
Children:
CVCL_0B38 (KM20-C)
Sex of cell Female
Age at sampling 44Y
Category Cancer cell line
STR profile Source(s): PubMed=25926053

Markers:
AmelogeninX
CSF1PO11,12
D5S81811,12
D7S82010
D13S31711
D16S53911,12
D21S1129,30
TH016
TPOX8,9
vWA17,19

Run an STR similarity search on this cell line
Web pages Info; ICLAC; -; https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx
Publications

PubMed=2846163
Kiyoshi Morikawa, Shirley M. Walker, Motowo Nakajima, Sen Pathak, John Milburn Jessup, Isaiah Joshua Fidler;
Influence of organ environment on the growth, selection, and metastasis of human colon carcinoma cells in nude mice.
Cancer Res. 48:6863-6871(1988)

PubMed=12060639
Qing-Ding Wang, Nan Li, Xiao-Fu Wang, Myung M. Kim, B. Mark Evers;
Augmentation of sodium butyrate-induced apoptosis by phosphatidylinositol 3'-kinase inhibition in the KM20 human colon cancer cell line.
Clin. Cancer Res. 8:1940-1947(2002)

PubMed=25926053; DOI=10.1038/ncomms8002
Enzo Medico, Mariangela Russo, Gabriele Picco, Carlotta Cancelliere, Emanuele Valtorta, Giorgio Corti, Michela Buscarino, Claudio Isella, Simona Lamba, Barbara Martinoglio, Silvio Veronese ...Show all 19 authors... , Salvatore Siena, Andrea Sartore-Bianchi, Marco Beccuti, Marcella Mottolese, Michael Linnebacher, Francesca Cordero, Federica Di Nicolantonio, Alberto Bardelli; Show fewer authors
The molecular landscape of colorectal cancer cell lines unveils clinically actionable kinase targets.
Nat. Commun. 6:7002.1-7002.10(2015)

Cross-references
Cell line collections (Providers) KCLB; 80017 - Discontinued
Cell line databases/resources cancercelllines; CVCL_L095
Biological sample resources BioSample; SAMN06481114
Encyclopedic resources Wikidata; Q54900064
Gene expression databases GEO; GSM1448074
Entry history
Entry creation06-May-2013
Last entry update10-Apr-2025
Version number24