ID   KM20
AC   CVCL_L095
SY   KM-20
DR   BioSample; SAMN06481114
DR   cancercelllines; CVCL_L095
DR   GEO; GSM1448074
DR   KCLB; 80017
DR   Wikidata; Q54900064
RX   PubMed=2846163;
RX   PubMed=12060639;
RX   PubMed=25926053;
WW   Info; ICLAC; -; https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx
CC   Problematic cell line: Contaminated. Shown to be a HT-29 derivative (ICLAC; an early sample of KM20 had been tested by the core facility where the cell line was established and the STR profile corresponds to that of HT-29).
CC   Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00513.
CC   Population: Caucasian.
CC   Microsatellite instability: Stable (MSS) (PubMed=25926053).
CC   Sequence variation: Mutation; HGNC; HGNC:583; APC; Simple; p.Glu853Ter (c.2557G>T); ClinVar=VCV001071632; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:583; APC; Simple; p.Thr1556Asnfs*3 (c.4666dupA) (c.4666_4667insA); ClinVar=VCV000428112; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.Pro449Thr (c.1345C>A); ClinVar=VCV001333250; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:6770; SMAD4; Simple; p.Gln311Ter (c.931C>T); ClinVar=VCV000230663; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg273His (c.818G>A); ClinVar=VCV000012366; Zygosity=Homozygous (from parent cell line).
CC   Omics: Transcriptomics; Microarray.
CC   Discontinued: KCLB; 80017; probable.
CC   Derived from site: In situ; Colon; UBERON=UBERON_0001155.
ST   Source(s): PubMed=25926053
ST   Amelogenin: X
ST   CSF1PO: 11,12
ST   D13S317: 11
ST   D16S539: 11,12
ST   D21S11: 29,30
ST   D5S818: 11,12
ST   D7S820: 10
ST   TH01: 6
ST   TPOX: 8,9
ST   vWA: 17,19
DI   NCIt; C4349; Colon adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0320 ! HT-29
SX   Female
AG   44Y
CA   Cancer cell line
DT   Created: 06-05-13; Last updated: 10-04-25; Version: 24
CH   CVCL_0B38 ! KM20-C
//
RX   PubMed=25926053; DOI=10.1038/ncomms8002;
RA   Medico, Enzo
RA   Russo, Mariangela
RA   Picco, Gabriele
RA   Cancelliere, Carlotta
RA   Valtorta, Emanuele
RA   Corti, Giorgio
RA   Buscarino, Michela
RA   Isella, Claudio
RA   Lamba, Simona
RA   Martinoglio, Barbara
RA   Veronese, Silvio
RA   Siena, Salvatore
RA   Sartore-Bianchi, Andrea
RA   Beccuti, Marco
RA   Mottolese, Marcella
RA   Linnebacher, Michael
RA   Cordero, Francesca
RA   Di Nicolantonio, Federica
RA   Bardelli, Alberto
RT   "The molecular landscape of colorectal cancer cell lines unveils
RT   clinically actionable kinase targets.";
RL   Nat. Commun. 6:7002.1-7002.10(2015).
//
RX   PubMed=2846163;
RA   Morikawa, Kiyoshi
RA   Walker, Shirley M.
RA   Nakajima, Motowo
RA   Pathak, Sen
RA   Jessup, John Milburn
RA   Fidler, Isaiah Joshua
RT   "Influence of organ environment on the growth, selection, and
RT   metastasis of human colon carcinoma cells in nude mice.";
RL   Cancer Res. 48:6863-6871(1988).
//
RX   PubMed=12060639;
RA   Wang, Qing-Ding
RA   Li, Nan
RA   Wang, Xiao-Fu
RA   Kim, Myung M.
RA   Evers, B. Mark
RT   "Augmentation of sodium butyrate-induced apoptosis by
RT   phosphatidylinositol 3'-kinase inhibition in the KM20 human colon
RT   cancer cell line.";
RL   Clin. Cancer Res. 8:1940-1947(2002).
//