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Cellosaurus BFH12 (CVCL_JQ51)

[Text version]
Cell line name BFH12
Synonyms Bovine Foetal Hepatocyte 12
Accession CVCL_JQ51
Resource Identification Initiative To cite this cell line use: BFH12 (RRID:CVCL_JQ51)
Comments Characteristics: Can be used for applications requiring a bovine hepatocyte-derived cell line with long cultivability and high reproducibility.
Senescence: SV40-transformed but with a finite life span. Dies out during passages 40-45.
Doubling time: 23.5 hours (Note=At 11th passage), 25.6 hours (Note=At 31th passage) (PubMed=27071625).
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=pRetro-E2 SV40).
Derived from site: In situ; Fetal liver; UBERON=UBERON_0002107.
Cell type: Hepatocyte; CL=CL_0000182.
Species of origin Bos taurus (Bovine) (NCBI Taxonomy: 9913)
Breed/subspecies: Holstein Friesian; VBO=VBO_0003171.
Hierarchy Children:
CVCL_D7FR (BFH12 CYP1A1 KO)CVCL_E7BR (BFH12 CYP3A74 KO)
Sex of cell Sex unspecified
Age at sampling Fetus
Category Finite cell line
Publications

PubMed=27071625; DOI=10.1007/s11626-016-0018-0
Gleich A., Kaiser B., Schumann J., Fuhrmann H.
Establishment and characterisation of a novel bovine SV40 large T-antigen-transduced foetal hepatocyte-derived cell line.
In Vitro Cell. Dev. Biol. Anim. 52:662-672(2016)

PubMed=30617848; DOI=10.1007/s10616-018-0279-4; PMCID=PMC6368520
Gleich A., Kaiser B., Honscha W., Fuhrmann H., Schoeniger A.
Evaluation of the hepatocyte-derived cell line BFH12 as an in vitro model for bovine biotransformation.
Cytotechnology 71:231-244(2019)

PubMed=38528259; DOI=10.1007/s10565-024-09856-7; PMCID=PMC10963470
Iori S., D'Onofrio C., Laham-Karam N., Mushimiyimana I., Lucatello L., Lopparelli R.M., Gelain M.E., Capolongo F., Pauletto M., Dacasto M., Giantin M.
Establishment and characterization of cytochrome P450 1A1 CRISPR/Cas9 knockout bovine foetal hepatocyte cell line (BFH12).
Cell Biol. Toxicol. 40:18.1-18.15(2024)

PubMed=38648904; DOI=10.1016/j.bcp.2024.116231
Iori S., D'Onofrio C., Laham-Karam N., Mushimiyimana I., Lucatello L., Montanucci L., Lopparelli R.M., Bonsembiante F., Capolongo F., Pauletto M., Dacasto M., Giantin M.
Generation and characterization of cytochrome P450 3A74 CRISPR/Cas9 knockout bovine foetal hepatocyte cell line (BFH12).
Biochem. Pharmacol. 224:116231.1-116231.11(2024)

Cross-references
Encyclopedic resources Wikidata; Q54796189
Entry history
Entry creation22-Aug-2017
Last entry update10-Apr-2025
Version number11